Vet Med - Czech, 2014, 59(6):299-306 | DOI: 10.17221/7574-VETMED

The field efficiency of oral rabies vaccination in the Lithuanian red fox population from 2006 to 2013Original Paper

D. Zienius1, G. Pridotkas2, I. Jaceviciene2, M. Ruzauskas1
1 Lithuanian University of Health Sciences, Institute of Microbiology and Virology, Kaunas, Lithuania
2 National Food and Veterinary Risk Assessment Institute, Vilnius, Lithuania

The objective of the present study was to assess the efficacy of an oral rabies vaccination (ORV) program in the period from 2006 to 2013 based on epidemiological data (Incidence of confirmed rabies in red foxes), biological marker evaluation (presence of tetracycline [TTC] indicating bait uptake), and the assessment of seroconversion (based on ELISA for rabies antibodies) in red foxes in Lithuania. Results were compared between juvenile and adult red foxes, and between the spring (March to May) and autumn (October to December) campaigns. On average, 20 baits/km2 were distributed over the entire territory of Lithuania. During the entire period of 2006-2013 1179 red foxes (10% of 11 829) were rabies-positive. While in 2006, 23.8% of tested foxes were rabies-positive, the incidence decreased to 2.4% in 2009, and 0.7% in 2011. Based on jaw bone investigation 78.1% of the foxes had ingested TTC from baits, 52.2% had seroconverted. The percentage of ELISA-positive red fox sera samples remained stable at 44.7-53.2% during both most recent ORV periods. The majority were adults (83%), 81.1 ± 8.54% of which were TCC-positive, and 73.8 ± 6.33% were TTC-positive juveniles. 52.5 ± 5.81% of adult red foxes had seroconversed, while 48.2 ± 4.51% of the young animals had a positive ELISA test in the years 2006 to 2013. This is in contrast to 2006 where only 29.5% of the young foxes were ELISA-positive. There were no significant differences between TTC- and ELISA-positive populations in different geographical regions of Lithuania.

Keywords: Baltic States; prevention; rabies; wildlife; TTC marker; ELISA seroconversion

Published: June 30, 2014  Show citation

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Zienius D, Pridotkas G, Jaceviciene I, Ruzauskas M. The field efficiency of oral rabies vaccination in the Lithuanian red fox population from 2006 to 2013. Vet Med - Czech. 2014;59(6):299-306. doi: 10.17221/7574-VETMED.
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    References

    1. Bourhy H, Rollin PE, Vincent J, Sureau P (1989): Comparative field evaluation of the fluorescent-antibody test, virus isolation from tissue culture, and enzyme immunodiagnosis for the rapid laboratory diagnosis of rabies. Journal of Clinical Microbiology 27, 519-523. Go to original source... Go to PubMed...
    2. Brochier B, Costy F, De Coninck V, Hallet L, Bourhy H, Peharpre D, Mosselmans F, Beyer R, Lecomte L, Mullier P, Bauduin B, Pastoret PP (1994): Epidemiosurveillance de la rage en Belgique: recrudescence en 1994. Annales de Médecine Vétérinaire 139, 263-273.
    3. Bruyere V, Vuillaume P, Cliquet F, Aubert M (2000): Oral rabies vaccination of foxes with one or two delayed distributions of SAG2 baits during the spring. Veterinary Research 31, 339-345. Go to original source... Go to PubMed...
    4. Cliquet F, Aubert M, Sagne L (1998): Development of a fluoresceent antibody virus neutralisation test (FAVN test) for the quantification of rabies-neutralising antibody. Journal of Immunological Methods 212, 79-87. Go to original source... Go to PubMed...
    5. Cliquet F, Muller T, Mutinelli F, Geronutti S, Brocherier B, Selhorst T, Shereffer JL, Kraft N, Burow J, Schameita A, Schuler H, Aubert M (2003): Standardization and establishment of rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns. Vaccine 21, 21-22. Go to original source... Go to PubMed...
    6. Cliquet F, Freuling C, Smreczak M, Van der Poel WHM, Horton D, Fooks AR, Robardet E, Picard-Meyer E, Muller T (2010): Development of harmonised schemes for monitoring and reporting of rabies in animals in the European Union. Scientific Report EFSA, 1-60. Go to original source...
    7. Cliquet F, Robardet E, Must K, Laine M, Peik K, PicardMeyer E, Guiot AL, Niin E (2012): Eliminating Rabies in Estonia. PLoS Neglected Tropical Diseases 6, e1535, DOI: 10.1371/journal.pntd.0001535. Go to original source... Go to PubMed...
    8. Dean DJ, Abelseth MK, Atanasiu P (1996): The fluorescent antibody test. In: Meslin FX, Kaplan MM, Koprowski H (eds.): Laboratory Techniques in Rabies. World Health Organization, Geneva. 88-95.
    9. De Benedictis P, Mancin M, Cattoli G, Capua I, Terrregino C (2012): Serological methods used for rabies post vaccination surveys: An analysis. Vaccine 30, 5611-5615. Go to original source... Go to PubMed...
    10. Freuling CM, Hampson K, Selhorst T, Schröder R, Meslin FX, Mettenleiter TC, Müller T (2013): The elimination of fox rabies from Europe: determinants of success and lessons for the future. Philosophical Transactions of the Royal Society B: Biological Sciences, 368, 20120142, http://dx.doi.org/10.1098/ rstb. 2012.0142. Go to original source... Go to PubMed...
    11. Hostnik P, Barlic-Maganja D, Toplak I, Grom J (2003): Persistence of rabies virus antibodies in the sera of fox cubs vaccinated with the vaccine Lysvulpen. Acta Veterinaria Brno 72, 207-212. Go to original source...
    12. Johnston DH, Joachim DG, Bachmann P, Kardong KV, Stewart REA, Dix LM, Strickland MA, Watt ID. (1987): Aging furbearers using tooth structure and biomarkers In: Nowak M BJ, Obbard ME, Malloch B (eds.): Wild Furbearer Management and Conservation in North America. Ontario Ministry of Natural Resources, Toronto, North Bay, Ontario, Canada. 228-243.
    13. Kauhala K (1996): Reproductive strategies of the raccoon dog and the red fox in Finland. Acta Theriologica 41, 51-58. Go to original source...
    14. Knoop EV, Freuling CM, Kliemt J, Selhorst T, Conraths FJ, Müller T (2010): Evaluation of a commercial rabies ELISA as a replacement for serum neutralization assays as part of the pet travel scheme and oral vaccination campaigns of foxes. Berliner und Münchener Tierärztliche Wochenschrift 123, 278-285. Go to PubMed...
    15. Lambot M, Blasco E, Barrat J, Cliquet F, Brochier B, Renders C, Krafft, N, Bailly J., Munier M., Aubert MFA, Pastoret, P-P (2001): Humoral and cellmediated immune responses of foxes (Vulpes vulpes) after experimental primary and secondary oral vaccination using SAG2 and VRG vaccines. Vaccine 19, 1827-1835. Go to original source... Go to PubMed...
    16. Matouch O, Vitasek J, Semerad Z, Malena M (2007): Rabies-free status of the Czech Republic after 15 years of oral vaccination. Revue Scientifique et Technique Office Internationale des Epizooties, 26, 577-584. Go to original source...
    17. Milius J, Razmuviene D, Jacevicius E, Tamosiunas V, Lukauskas K (2004): Epidemiological situation of rabies in Lithuania in 1994-2003. Veterinarija ir Zootechnika 27, 11-18.
    18. Rosatte RC, Lawson KF (2001): Acceptance of baits for delivery of oral rabies vaccine to raccoons. Journal of Wildlife Diseases 37, 730-739. Go to original source... Go to PubMed...
    19. Rosatte R, Allan M, Bachmann P, Sobey K, Donovan D, Davies JC, Silver A, Benett K, Brown L, Stevenson B, Buchanan T, Bruce L, Wandeler A, Fehlner-Gardiner C, Beresford A, Beath A, Escobar M, Maki J, Schumacher C. (2008): Prevalence of tetracycline and rabies virus antibody in raccoons, skunks, and foxes following aerial distribution of V-RG baits to control raccoon rabies in Ontario, Canada. Journal of Wildlife Diseases 44, 946-964. Go to original source... Go to PubMed...
    20. Selhorst T, Müller T, Schwermer HP, Ziller M, Schluter H (2005): Use of an area index to retrospectively analyze the elimination of fox rabies in European Countries. Environmental Management 35, 292-302. DOI:10.1007/s00267-003-3055-x). Go to original source... Go to PubMed...
    21. Servat A, Feyssaguet M, Blanchard I, Morize JL, Schereffer JL, Boue F, Cliquet F (2007): A quantitative indirect ELISA to monitor the effectiveness of rabies vaccination in domestic and wild carnivores. Journal of Immunological Methods 318, 1-10. Go to original source... Go to PubMed...
    22. Servat A, Labadie A, Hamen A, Boue F, Cliquet F (2008): Inter-laboratory trial to evaluate the reproducibility of a new ELISA to detect rabies antibodies in vaccinated domestic and wild carnivores. Biologicals 36, 19-26. Go to original source... Go to PubMed...
    23. Singer A, Kauhala K, Holmala K, Smith GC (2009): Rabies in Northeastern Europe - the threat from invasive raccoon dogs. Journal of Wildlife Diseases 45, 1121-1137. Go to original source... Go to PubMed...
    24. Smith JS, Yager PA, Baer GM (1996): A rapid fluorescent focus inhibition test (RFFIT) for determining rabies virusneutralising antibody. In: Meslin FX, Kaplin MM, Koprowski H (eds.): Laboratory Techniques in Rabies. 4th ed. World Health Organization, Geneva. 181-189.
    25. Wandeler AI (2008): The rabies situation in Western Europe. Towards the Elimination of Rabies in Eurasia. Developments in Biology, Basel Karger 131, 19-25.
    26. Wasniewski M, Guiot AL, Schereffer JL, Tribout L, Mahar K, Cliquet F (2013): Evaluation of an ELISA to detect rabies antibodies in orally vaccinated foxes and raccoon dogs sampled in the field. Journal of Virological Methods 187, 264-270. Go to original source... Go to PubMed...

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