Vet Med - Czech, 2023, 68(2):62-68 | DOI: 10.17221/59/2022-VETMED

Presence of zoonotic black-pigmented periodontal pathogens in the oral microbiota of pet and stray catsOriginal Paper

V Ozavci1, HTY Dolgun2, S Kirkan2
1 Department of Microbiology, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, Türkiye
2 Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Türkiye

Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk to humans. In this study, we aimed to determine the presence of Porphyromonas gingivalis, Porphyromonas gulae and Prevotella nigrescens in the oral microbiota of pet and stray cats. Dental swab samples were taken from 25 pet cats and 25 stray cats with symptoms of periodontal disease and then investigated by multiplex polymerase chain reaction using 16S rRNA species-specific primers. As a result of the multiplex PCR analysis, P. gingivalis 3/25 (12%), P. nigrescens 1/25 (4%), P. gingivalis + P. gulae 7/25 (28%), P. gingivalis + P. nigrescens 1/25 (4%), P. gulae + P. nigrescens 1/25 (4%), and P. gingivalis + P. gulae + P. nigrescens 2/25 (8%) were molecularly typed in the pet cats. In addition, 1/25 (4%) of P. gulae and 21/25 (84%) of P. gingivalis + P. gulae were typed in the stray cats. In 10/25 (40%) pet and 3/25 (12%) stray cat samples, no bacteria were detected by molecular typing. In summary, the results provide strong evidence that black-pigmented zoonotic pathogens are associated with cat periodontal disease.

Keywords: PCR; Porphyromonas gingivalis; Porphyromonas gulae; Prevotella nigrescens

Received: July 7, 2022; Accepted: January 10, 2023; Prepublished online: February 20, 2023; Published: February 24, 2023  Show citation

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Ozavci V, Dolgun H, Kirkan S. Presence of zoonotic black-pigmented periodontal pathogens in the oral microbiota of pet and stray cats. Vet Med - Czech. 2023;68(2):62-68. doi: 10.17221/59/2022-VETMED.
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