Vet Med - Czech, 2024, 69(9):321-328 | DOI: 10.17221/116/2023-VETMED

Molecular detection and characterisation of Mycoplasma species in community owned dogs of Kerala, a South Indian StateOriginal Paper

GS Kumar1, A Varghese ORCID...1, PK Hembram1, KGA Kumar ORCID...1, CK Deepa ORCID...1, A Varghese2, R Ravindran ORCID...1
1 Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
2 Veterinary Consultant, Zootopia Veterinary Clinic, Chalakudy, Kerala, India

Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of Mycoplasma organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of Mycoplasma spp. in dogs of different zones of Kerala was attempted using 16S rRNA gene. An overall prevalence of 18% for M. haemocanis was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of M. haemocanis. The phylogenetic analysis revealed clustering of M. haemocanis and M. haemofelis in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis.

Keywords: 16S rRNA; dogs; haplotype networks; Mycoplasma haemocanis; phylogenetic analysis

Received: November 10, 2023; Revised: August 5, 2024; Accepted: August 12, 2024; Published: September 29, 2024  Show citation

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Kumar G, Varghese A, Hembram P, Kumar K, Deepa C, Varghese A, Ravindran R. Molecular detection and characterisation of Mycoplasma species in community owned dogs of Kerala, a South Indian State. Vet Med - Czech. 2024;69(9):321-328. doi: 10.17221/116/2023-VETMED.
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