Veterinární medicína, 2001 (vol. 46), issue 1

Effect of vitamin E and selenium on blood glutathione peroxidase activity and some immunological parameters in sheepOriginal Paper

K. Milad, G. Kováč, O. Rácz, A. Šipulová, V. Bajová

Vet Med - Czech, 2001, 46(1):1-5 | DOI: 10.17221/7843-VETMED  

The objective of this study was to determine the effect of vitamin E and selenium administration on glutathione peroxidase (GSH-Px) activity and selected parameters of cellular immunity (metabolic activity, phagocytic activity, lymphocyte blastogenic response). Nine pregnant sheep with body weight of 42 to 66 kg were divided into two groups. Before lambing, the first group (n = 5) was given subcutaneously 5 mg vitamin E and 0.4 mg selenium per kg body weight while the second group (n = 4) was given no treatment and served as control. Blood samples from all sheep were collected before the treatment, 14 days after lambing and 30 days...

Resistance in carps (Cyprinus carpio L.) affected by a natural bacterial infectionOriginal Paper

M. Stosik, W. Deptula, M. Trávniček

Vet Med - Czech, 2001, 46(1):6-11 | DOI: 10.17221/7844-VETMED  

Sick carps, affected by acute or chronic forms of erythrodermatitis (CE) were studied. The studies were aimed at obtaining pathophysiological data which would permit evaluation of clinical status and prognosis in carps of various age (23 or 28 months). This was approached by estimation of the number of carps and of dynamics of changes in nonspecific humoral and cell mediated resistance and in levels of selected serum proteins. The ingesting index of neutrophilic granulocytes and percent of neutrophilic granulocytes capable of ingesting proved significantly higher in sick carps as compared to the healthy carps, at every age of carps and form of the...

Studies on the number and ingesting ability of thrombocytes in sick carps (Cyprinus carpio L.)Original Paper

M. Stosik, W. Deptula, M. Trávniček

Vet Med - Czech, 2001, 46(1):12-16 | DOI: 10.17221/7845-VETMED  

Carps suffering from acute form of brachiomycosis (age: 2 months), chronic form of brachionecrosis cyprinorum (age: 12 months), acute form of erythrodermatitis (age: 23 and 28 months) or chronic form of erythrodermatitis (age: 23 months) were studied. The level of thrombocytes in carps with acute forms of the diseases was significantly lower than that in healthy carps. The inverse pattern was observed in carps with chronic forms of the diseases. Carps suffering from the acute form of erythrodermatitis (age: 23 and 28 months) demonstrated no differences in the level of thrombocytes which could be related to age or body weight of the animals. Index of...

Estrogenic effects of silymarin in ovariectomized ratsOriginal Paper

V. Kummer, J. Mašková, J. Čanderle, Z. Zralý, J. Neča, M. Machala

Vet Med - Czech, 2001, 46(1):17-23 | DOI: 10.17221/7846-VETMED  

The objective of this study was to test whether silymarin induces changes indicative of estrogenic effects in gonadal organs of ovariectomized (OVX) rats. Silymarin was administered in two experimental groups of OVX rats (n = 7 + 7) for 30 days at the doses of 25 or 50 mg per animal per day. OVX rats (n = 7) receiving 5 µg of 17b-estradiol (E2) for the last three days before killing and untreated OVX rats (n = 7) were used as the positive and the negative controls, respectively. Uterine and blood samples were collected immediately after killing. Compared with the negative controls, total and normalized uterine weights...

Staphylococci plate counts in foods of milk originOriginal Paper

E. Beličková, Ľ. Tkáčiková, T. H Naas, M. Vargová, M. Ondrašovič, O. Ondrašovičová, D. Obšitníková, L. Tóth

Vet Med - Czech, 2001, 46(1):24-27 | DOI: 10.17221/7847-VETMED  

We have examined 35 samples of fine cottage cheese, 14 samples of whole winter "bryndza", 29 samples of Ondava cheese, 18 samples of skim kephir milk, 18 samples of whole acidophilous milk, 5 samples of yoghurt milk with strawberry flavour and 50 samples of fresh butter (Rajo) for the presence of staphylococci focusing onStaphylococcus aureus. All samples intended for microbiological analysis were taken directly from market establishments. None of the examined samples of yoghurt milk complied with the Codex Alimentarius because they contained Staphylococcus aureus bacteria on the level of 100, 65, 5, 75, and 60 CFU/ml. The other analysed...