Veterinární medicína, 2003 (vol. 48), issue 11
Effects of some antibiotics on glutathione reductase from bovine erythrocytes
M. Erat, H. Sakiroglu, M. Ciftci
Vet Med - Czech, 2003, 48(11):305-312 | DOI: 10.17221/5784-VETMED 
This paper describes effects of some antibiotics on glutathione reductase in vitro. Glutathione reductase was purified from bovine erythrocytes. Purification procedure consisted of four steps; preparation of hemolysate, ammonium sulphate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G-200 gel filtration chromatography. As a result of these four consecutive procedures, the enzyme having the specific activity of 62.5 EU/mg proteins was purified 31 250-fold with a yield of 11.39%. The purified enzyme showed a single band on sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE). The effects of eight...
Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study)
V. Koonradova, J. Uhlik, L. Vajner, J. Herget, J. Adaskova
Vet Med - Czech, 2003, 48(11):313-320 | DOI: 10.17221/5785-VETMED
The ultrastructure of the tracheal epithelium in rabbits exposed for 96 hours to 35-37% O2 was studied in our experiments. Due to the influence of mild normobaric hyperoxia, massive differentiation of new secretory elements was initiated and resulted in apparent changes in goblet cells distribution. 60 ± 4% of goblet cells took part in the formation of voluminous intraepithelial mucous glands. Ciliated cells were less damaged than the goblet ones. Tiny signs of pathological alteration of deeper portions of their cytoplasm and apical blebbing accompanied with destruction of some kinocilia were encountered. The ciliary border was slightly impaired....
A serological survey and isolation of leptospires from small rodents and wild boars in the Republic of Croatia
Z. Cvetnic, J. Margaletic, J. Toncic, N. Tturk, Z. Milas, S. Spicic, M. Lojkic, S. Terzic, L. Jemersic, A. Humski, M. Mitak, B. Habrun, B. Krt
Vet Med - Czech, 2003, 48(11):321-329 | DOI: 10.17221/5786-VETMED
In total the samples of blood and kidneys of 379 small rodents and 154 wild swine were analysed. The antibodies to different serovars of leptospires were determined in 12.7% of small rodents, most often in the species Mus musculus (34.4%), A. agrestis (14.8%), A. flavicolis (10.8%), C. glareolus (9.4%) and A. sylvaticus (6.5%). Most frequent were the findings of antibodies to sv. pomona (27.1%), sv. sejroe (20.8%), and sv. australis (14.6%), and the antibodies to sv. hardjo, sv. saxkoebing, sv. tarassovi, sv. grippotyphosa, sv. bataviae...
The prevalence of and resistance to antimicrobial agents of Bacillus cereus isolates from foodstuffs
J. Schlegelova, J. Brychta, E. Klimova, E. Napravnikova, V. Babak
Vet Med - Czech, 2003, 48(11):331-338 | DOI: 10.17221/5787-VETMED
The study was aimed at the assessment whether foodstuffs contaminated with Bacillus cereus (B. cereus) may concurrently be vectors of spreading resistance. The contamination of foodstuffs with B. cereus strains was found in 31% of dairy and in 28% of meat products tested. Only one product from skimmed milk was contaminated. High-fat milk products that were heat-treated during the technological process (87 samples), as well as heat-treated meat products (65 samples), were contaminated significantly frequently (63% and 48% of the samples respectively) (P < 0.01). Almost all B. cereus isolates displayed...
Treatment of a case of tracheal stenosis in a dog with tracheal resection and anastomosis
Z. Mutlu, S.E. Acar, C. Perk
Vet Med - Czech, 2003, 48(11):339-342 | DOI: 10.17221/5788-VETMED
A case of tracheal stenosis in the cervical portion of the trachea was encountered in a 5.5-month-old St. Bernard-Roweiler cross dog. Breathing difficulty was seen in the clinical examination and presence of an obvious narrowing between the 3rd-5th cervical tracheal rings was determined in the radiological examination. Under general anesthesia the portion with stenosis was resected and the healthy trachea ends were anastomosed using the split cartilage technique. In the postoperative period the breathing difficulty disappeared and there was no development of a new stenosis in the anastomosis region. In the late period check-up the patient...