Veterinární medicína, 2003 (vol. 48), issue 9
Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetic acid hapten using naďve phage library
J. Brichta, H. Vesela, M. Franek
Vet Med - Czech, 2003, 48(9):237-247 | DOI: 10.17221/5776-VETMED 
Three single chain variable fragment (scFv) antibodies against 2,4-dichlophenoxyacetic acid (2,4-D) herbicide were produced by the Griffin1.library. The selection of the scFv from the phage library was carried out by 2,4-D-protein coated tubes with different levels of hapten substitution in the conjugate. The scFv phage clones were isolated within the five round library panning and the antibodies were expressed in Escherichia coli HB2151. The recombinant products were purified by metal affinity chromatography yielding 200 g of pure scFv per 1 liter of bacterial culture. The antibody fragments provided steep curves in conventional indirect...
impact of manual and mechanical cut-up of broiler carcasses on the enterprise income
Y. Cevger, S. Sariozkan, H. Guler
Vet Med - Czech, 2003, 48(9):248-253 | DOI: 10.17221/5777-VETMED
This research was carried out in the Meat and Fish Products Co. Sincan Plant (Ankara,Turkey) between March 2002-May 2002. Total 666 broiler carcasses, 461 of which was cut-up with hand using a knife and 205 of which was cut up with cutting saw, formed the research material. Cut-up carcasses were classified in 6 groups according to their weights. The carcasses were weighted as a whole in both methods and after their weights were recorded, they were cut-up into 4 parts as breast, legs, wings and neck. It was determined that process of cutting up with knife lasted for 60 s and for 17 s with cutting saw. As a result of cutting up, net income increase was...
The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein
V. Valentova
Vet Med - Czech, 2003, 48(9):254-266 | DOI: 10.17221/5778-VETMED
Bovine respiratory syncytial virus (BRSV) and related human respiratory syncytial virus (HRSV) are major respiratory tract pathogens in calves and infants, respectively. Great attention is now paid to prevention of the disease caused by these agents. Glycoprotein G is the most variable viral protein and antigenic grouping of RSV isolates is based on distinct antigenic reactivity patterns determined with a set of G protein specific mAbs. Genetic variability of the G protein is used during epidemiology and epizootiology studies of HRSV and BRSV diseases, respectively. The constant genetic drift can be observed within G protein sequences. Both cell-mediated...