Veterinární medicína, 2004 (vol. 49), issue 1
Bull and boar sperm DNA integrity evaluated by sperm chromatin structure assay in the Czech Republic
R. Rybak, L. Faldikova, M. Faldyna, M. Machatkova, J. Rubes
Vet Med - Czech, 2004, 49(1):1-8 | DOI: 10.17221/5668-VETMED 
Analysis of sperm parameters is very important for predicting the outcome of assisted reproductive techniques and is necessary for determination of fertility potential of males tested for artificial insemination. In our study we have determined the level of bull and boar sperm DNA damage by Sperm Chromatin Structure Assay (SCSA). This test is based on increased susceptibility of altered DNA (strand breaks) in sperm nuclear chromatinto in situ denaturation measured by flow cytometry after staining with acridine orange (AO). Sperm chromatin damage was quantified by percentages of spermatozoa with detectable DNA Fragmentation Index - DFI divided...
Prevalence of antibodies to Toxoplasma gondii in horses in the province of Kars, Turkey
A. Akca, C. Babur, Arslan MO, Y. Gicik, M. Kara, S. Kilic
Vet Med - Czech, 2004, 49(1):9-13 | DOI: 10.17221/5667-VETMED
This study investigates the seroprevalence of Toxoplasma gondii in horses from seven villages in the province of Kars in north-eastern Turkey. A total of 189 serum samples from clinically healthy, local crossbred horses were tested for anti-T. gondii antibodies using the Sabin-Feldman dye test. Antibodies to T. gondii were found in 39 (20.6%) horses, with specific titres of 1 : 16 (27), 1 : 64 (11) and 1 : 256 (1). The 95% confidence interval for the population proportion ranged from 13.3 to 27.9%. The number of seropositive horses in Yucelen village (40%) was considerably higher than in other villages, whereas in the villages...
Spatio-temporal aspects of tularemia in Southern Moravia, Czech Republic
J. Piskula, M. Beklova, Z. Holesovska, F. Treml
Vet Med - Czech, 2004, 49(1):15-18 | DOI: 10.17221/5670-VETMED
In the last decade, apart from the rise of numbers of positive hares and infected humans, we have been witnessing some spread of natural foci of tularemia into more northern areas of Southern Moravia, Czech Republic. It was, therefore, the aim of this paper to evaluate the spatio-temporal development of tularemia in Southern Moravia (in a selected study area of 130 × 90 km) using a geographic information system. Correlation analysis resulted in finding that the geographic distribution of natural foci of tularemia in any year correlated with the distribution in any other year of the study period of 1994-2001. The coefficients of correlation of all possible...
A review of the diseases and contaminant related mortalities of tench (Tinca tinca L.)
Z. Svobodova, J. Kolarova
Vet Med - Czech, 2004, 49(1):19-34 | DOI: 10.17221/5671-VETMED
The paper provides a review of the current state of knowledge of parasites, diseases and poisonings of tench (Tinca tinca L.) based on the literature and on the authors own findings. Information on the viruses, bacteria, fungi and parasites reported from tench are provided along with deaths resulting from alterations in environmental quality. Finally, a list of the current known species of parasites and diseases reported in the Czech Republic are provided based on our own investigations carried out at the Research Institute of Fish Culture and Hydrobiology at Vodňany (University of South Bohemia Ceske Budejovice, Czech Republic) between 1984...