Veterinární medicína, 2004 (vol. 49), issue 9
Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs
J. Osek
Vet Med - Czech, 2004, 49(9):317-326 | DOI: 10.17221/5711-VETMED 
A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as for antimicrobial resistance and biochemical properties. Fourteen of the human strains (n = 23) and 21 of the bovine isolates (n = 29) were of the O157:H7 serotype as determined by agglutination and PCR methods. All E. coli O157 of porcine origin (n = 38) were H-negative. Based on the ability to produce Shiga toxins (Stxs), all human and cattle isolates and 11 strains recovered from swine were identified as Shiga...
Purification and characterization of glucose 6-phosphate dehydrogenase from rainbow trout (oncorhynchus mykiss) erythrocytes
M. Ciftci, A. Ciltas, O. Erdogan
Vet Med - Czech, 2004, 49(9):327-333 | DOI: 10.17221/5712-VETMED
Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) from rainbow trout (Oncorhynchus mykiss) erythrocytes was purified, using a simple and rapid method, and some characteristics of the enzyme were investigated. The purification procedure consisted of three steps: haemolysate preparation, ammonium sulphate precipitation, and 2',5'-ADP Sepharose 4B affinity gel chromatography, which took one working day. Thanks to the three consecutive procedures, the enzyme, having the specific activity of 14.51 EU/mg proteins, was purified with a yield of 70.40% and 1 271.19-fold. In order to control...
Immunohistochemical determination of calbindin-d28k in the kidney of postnatal rats
S. Timurkaan, B. G Tarakci
Vet Med - Czech, 2004, 49(9):334-338 | DOI: 10.17221/5713-VETMED
The localization of calbindin-D28k was examined in rat kidney at different stages of development. Calbindin was found specifically in the distal convoluted tubules (DCT). In the 10 day old rat kidney many cells apparently belonging to the collecting tubules were positive. In the neonatal nephrons there was also localization of calbindin-D28k to the limb of Henle, suggesting a difference in the regulation of intracellular calcium during maturation. Proximal tubule, renal corpuscle and macula densa gave no specific localization of calbindin-D28k.
A case of anterior iris cyst in an akbash dog
M. Saroglu, K. Altunatmaz, Y. Devecioglu
Vet Med - Czech, 2004, 49(9):339-342 | DOI: 10.17221/5714-VETMED
In this case presentation, an uncomplicated anterior iris cyst encountered in an 8-year-old female Akbash dog has been discussed. While these structures may be congenital, they may also develop secondarily due to trauma or chronic inflammation. Following systemic examination, the iris cyst was diagnosed to be present in the 5-6 o'clock position and that it was attached to the pupillary margin on the anterior surface of the iris. There were no abnormalities in any of the intraocular structures. No intervention of treatment was made, since the anterior iris cyst in the patient did not cause either corneal opacity or an increase in intraocular pressure.
Fish: a potential source of bacterial pathogens for human beings
L. Novotny, L. Dvorska, A. Lorencova, V. Beran, I. Pavlik
Vet Med - Czech, 2004, 49(9):343-358 | DOI: 10.17221/5715-VETMED
Human infections caused by pathogens transmitted from fish or the aquatic environment are quite common and depend on the season, patients' contact with fish and related environment, dietary habits and the immune system status of the exposed individual. They are often bacterial species facultatively pathogenic for both fish and human beings and may be isolated from fish without apparent symptoms of the disease. The infection source may be fish kept for both for food and as a hobby. Human infections and intoxications with the following bacteria have been recorded: Mycobacterium spp., Streptococcus iniae, Photobacterium damselae,...