Veterinární medicína, 2005 (vol. 50), issue 1
Antibody based methods for environmental and food analysis: a review
M. Franek, K. Hruska
Vet Med - Czech, 2005, 50(1):1-10 | DOI: 10.17221/5591-VETMED 
Antibodies have widely been used as analytical tools in various assays and techniques developed for clinical chemistry and endocrinology and for food and environmental research and risk control. Antibody development in the Veterinary Research Institute, Brno, and their application in ELISA and related techniques such as immunosensors has been directed especially to phenoxyacetic acid herbicides, s-triazine herbicides, sulfonylurea herbicides, polychlorinated biphenyls, surfactants (linear alkylbenzene sulphonates) and toxic metabolites (nonylphenol), and selected veterinary drugs (namely nitrofurans and sulfonamides). This paper provides an overview...
Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland neutrophils in vitro
Z. Sladek, D. Rysanek, M. Faldyna
Vet Med - Czech, 2005, 50(1):11-23 | DOI: 10.17221/5592-VETMED
Neutrophils play an important role in the defence of the bovine mammary gland against bacterial infections. In the course of the resolution of mammary gland inflammation, neutrophils undergo programmed cell death - apoptosis. The aim of this study was to confirm whether the co-cultivation of neutrophils of the bovine mammary gland with either Staphylococcus aureus or Streptococcus uberis leads to signs of apoptosis. In the study, 16 mammary glands of four virgin heifers aged 16 to 18 months were examined. Neutrophils were obtained by lavage after an induced influx. After a three-hour incubation of the neutrophils with bacteria...
Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer
M. Hidalgo, I. Rodriguez, J. Dorado, J. Sanz, C. Soler
Vet Med - Czech, 2005, 50(1):24-32 | DOI: 10.17221/5593-VETMED
Computer-assisted sperm morphometry analysis has improved the assessment of sperm morphology, but the results depend on the use of adequate evaluation and staining procedures of spermatozoa from individual species. In this study, the morphological module of the Sperm Class Analyzer®was used for the morphometric analysis of stallion sperm heads and midpieces. Semen samples were obtained from six fertile stallions in order to evaluate the influence of three staining procedures (Diff-Quik, Hemacolor and Harris' Haematoxylin) on the accuracy of image processing and sperm morphometry, and the effect of the sample size on sperm morphometric measurements....
Estrous synchronization during the natural breeding season in Anatolian black does
I. Dogan, Z. Nur, U. Gunay, H. Sagirkaya, M.K. Soylu, C. Sonmez
Vet Med - Czech, 2005, 50(1):33-38 | DOI: 10.17221/5594-VETMED
The efficiency of medroxyprogesterone acetate (MAP) and fluorogestone acetate (FGA) sponges with or without PGF2α (cloprostenol) for synchronizing estrous in non-lactating does was investigated during the natural breeding season. Does were treated for 11 days with 60 mg MAP (n = 38) or 40 mg FGA (n = 32) sponges. All does also received intramuscular injections of 500 IU PMSG. In addition, 19 and 14 of the does synchronized with MAP and FGA respectively, were injected with 125 μg cloprostenol and the remaining does from both groups were injected with 1.5 ml of sterile saline solution, 48 h prior the sponge removal....
Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment
S. Martinez-Subiela, J.J. Ceron
Vet Med - Czech, 2005, 50(1):39-46 | DOI: 10.17221/5595-VETMED
An acute phase index based on a combination of acute phase proteins which permitted monitoring the response to therapy of canine leishmaniasis was developed and evaluated in this study. Six dogs naturally infected by Leishmania infantum were treated with meglumine antimoniate (Glucantime, Merial, Lyon, France) 100 mg/kg/day sc, given concurrently for 20 days with allopurinol (Zyloric®, Glaxo Wellcome, Madrid, Spain) 30 mg/kg/day po and then, allopurinol alone for one month at the same dosage. Blood samples for acute phase proteins were obtained on different days before and after the beginning of treatment...