Veterinární medicína, 2005 (vol. 50), issue 4
Current knowledge on koi herpesvirus (KHV) - a review
D. Pokorova, T. Vesely, V. Piackova, S. Reschova, J. Hulova
Vet Med - Czech, 2005, 50(4):139-148 | DOI: 10.17221/5607-VETMED 
The first outbreaks of a disease connected with high mortality of common carp and koi carp caused by koi herpesvirus (KHV) were reported in 1998 in Israel and in the United States. Since then, several cases have been confirmed all over the world. At present, this viral disease is considered to be one of the most risky factors affecting populations of common carp and koi carp. Affected fish become disoriented and swim erratically with high breathing frequency, swollen gills and partially local skin lesions. The virus was isolated from the tissues of fish showing signs of the disease and subsequently cultured on koi fin (KF-1) cells. Electron microscopic...
In vivo culture of bovine embryos and quality assessment of in vivo vs. in vitro produced embryos
V. Havlicek, M. Lopatarova, S. Cech, R. Dolezel, T. Huber, A. Pavlok, G. Brem, U. Besenfelder
Vet Med - Czech, 2005, 50(4):149-158 | DOI: 10.17221/5608-VETMED
Routine access to the bovine oviduct for in vivo culture accomplishes various demands on embryo production for scientific as well as commercial purposes. The experiments conducted in the present study focused on the efficiency of recovery methods after temporary in vivo culture of bovine embryos in oviducts of the homologous species using transvaginal endoscopy (Experiment I) and on the quality assessment of recovered blastocysts (Experiment II). In Experiment I in vitro matured oocytes were fertilized, cultured for 1 to 3 days and transferred unilaterally into the ipsilateral oviducts of 54 heifers by the means of transvaginal...
Occurrence of the coccidium Isospora suis in piglets
K. Hamadejova, J. Vitovec
Vet Med - Czech, 2005, 50(4):159-163 | DOI: 10.17221/5610-VETMED
In the period October 2002-September 2004 we examined 2 996 samples of faeces of piglets at the age of 2-47 days. Samples were collected as so called "composite" ones from the pen floor. Samplings were done in 8 herds in Ceske Budejovice district. One herd was kept on the slatted floor, the other herds were housed on litter. Coprological examinations were carried out within 24 hours after sampling, and Sheather's sugar solution was used. Average prevalence of the coccidium Isospora suis was 24.8%. Isosporosis prevalence in piglets was highest on day 13 of piglet age (46.3%) and at week 2 after birth (38.8%). With respect to seasonal dynamics...
Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary
N. Nogrady, I. Gado, P. Zsolt Fekete, J. Paszti
Vet Med - Czech, 2005, 50(4):164-170 | DOI: 10.17221/5609-VETMED
The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar Typhimurium strains isolated from animals and humans in Hungary, by PCR and conjugation. Three groups of chloramphenicol resistant strains were identified. Eleven animal and 13 human isolates harboured the flo gene, encoding resistance to chloramphenicol and florfenicol, and possessed integrons of 1.0 Kb and 1.2 Kb typically found on the multidrug resistance island of S. Typhimurium...
Prevalence of thermophilic Campylobacter spp. in slaughtered pigs in the Czech Republic, 2001-2003
I. Steinhauserova, M. Nebola, M. Mikulicova
Vet Med - Czech, 2005, 50(4):171-174 | DOI: 10.17221/5611-VETMED
The prevalence of thermophilic Campylobacter spp. was evaluated in the caecum and on carcasses of pigs at slaughter and in the facilities of slaughterhouses in the period of 2001- 2003. During that timeframe, prevalence of Campylobacter spp. in both the pigs and the environment of slaughterhouses decreased. In 2001, Campylobacter spp. were detected in 34% of 316 samples; in 2002 there were 27% of positive findings out of the 624 samples; and in 2003, Campylobacter spp. were detected in 16% out of 300 samples. Campylobacter spp. were mostly found primarily in the caecum (292 isolates) and in smears collected...
Characterization of genetic diversity of animal and human Mycobacterium avium strains by IS1245-IS1311 spacer typing
M. Pate, M. Ocepek, M. Zolnir-Dovc, B. Krt
Vet Med - Czech, 2005, 50(4):175-180 | DOI: 10.17221/5612-VETMED
A PCR method previously developed for typing Mycobacterium avium was used to characterize the genetic diversity of M. avium strains isolated from swine (n = 90) and humans (n = 24). The strains were identified with IS901 PCR and IS1245 PCR: 38 strains were of IS901+ and IS1245+ genotype (M. avium subsp. avium) and 76 strains were of IS901- and IS1245+ genotype (M. avium subsp. hominissuis). All human isolates were IS901 negative. IS1245-IS1311 spacer typing was successfully accomplished for 59 isolates...