Veterinární medicína, 2007 (vol. 52), issue 3
Effect of unsaturated C18 fatty acids (oleic, linoleic and α-linolenic acids) on ruminal fermentation and production of fatty acids isomers in artificial rumen
D. Jalc, M. Certik, K. Kundrikova, P. Namestkova
Vet Med - Czech, 2007, 52(3):87-94 | DOI: 10.17221/2012-VETMED 
The objective of this study was to examine the effect of oleic (OA), linoleic (LA) and α-linolenic (ALA) acid used as supplements (3.5% wt/wt) to a diet containing 80% lucerne and 20% barley on rumen fermentation and lipid metabolism in an artificial rumen (Rusitec). The experiment lasted 12 days with 6 days of stabilization period. The fatty acid (FA) supplementation to a mixed diet did not affect any parameters of rumen fermentation (degradation of DM, NDF, ADF, total VFA production, production of acetate, propionate and butyrate). The methane production was decreased numerically (NS) by FA supplements (OA, LA, and ALA by about 8, 8.3 and 13.2%,...
Dose dependent changes in inflammatory parameters in the milk of dairy cows after intramammary infusion of lipopolysaccharide
C. Werner-Misof, J. Macuhova, V. Tancin, R.M. Bruckmaier
Vet Med - Czech, 2007, 52(3):95-102 | DOI: 10.17221/1870-VETMED
The goal of this study was to evaluate the dose dependent changes in common milk and blood parameters for udder health after an intramammary (IM) infusion of five different doses of lipopolysaccharide (LPS, 100, 50, 25, 12.5 and 6.25 μg, respectively). Ten Holstein Friesian cows randomly divided into five groups of 2 cows each were IM infused into one quarter with one LPS dose dissolved in 10 ml of saline. The contralateral quarter was infused with 10 ml saline (9 g/l). Milk samples were taken immediately before and 12, 24, 36, 48 and 60 hours after the treatment. All milk samples were analysed for somatic cell counts (SCC), lactose, sodium (Na),...
Effects of 2-phenoxyethanol anaesthesia on sheatfish (Silurus glanis L.)
J. Velisek, T. Wlasow, P. Gomulka, Z. Svobodova, L. Novotny
Vet Med - Czech, 2007, 52(3):103-110 | DOI: 10.17221/2011-VETMED
The aim of the study was to investigate the acute toxicity of 2-phenoxyethanol to sheatfish, and using the values of haematological and biochemical profiles of blood and histological tissue examinations to assess the effects of the fish exposure to that anaesthetic. The values of acute toxicity of 2-phenoxyethanol to sheatfish were found to be 10minLC50 0.77 ml/l, 10minLC0.1 0.42 ml/l, 10minLC99.9 1.90 ml/l, 96hLC50 0.29 ml/l, 96hLC0.1 0.20 ml/l, and 96hLC99.9 0.41 ml/l. The 10-min exposure to 2-phenoxyethanol at a concentration of 0.30 ml/l caused significantly higher values (P < 0.05) of packed cell volume (PCV), mean corpuscular volume...
Determination of volatile organic compounds in the crude and heat treated amaranth samples
M. Ciganek, B. Pisarikova, Z. Zraly
Vet Med - Czech, 2007, 52(3):111-120 | DOI: 10.17221/1869-VETMED
The present study concentrated on the development of an analytical method for determination of emissions of volatile organic compounds from crude and heat treated amaranth (genus Amaranthus L.) samples. Emitted substances were collected by solid-phase microextraction (SPME) method and identified by gas chromatography with mass spectrometry. The list of identified abundant organic compounds exceeds one hundred substances of different classes. Total concentrations of quantified volatile organic compounds ranged between 2.2 and 68.9 μg/g of dried sample. Hexanal and acetic acid were found as the most abundant compounds detected in amaranth samples....
Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage
D. Kosakova, P. Scheer, J. Lata, J. Doubek
Vet Med - Czech, 2007, 52(3):121-129 | DOI: 10.17221/2013-VETMED
The aim of the study was evaluate the influence of the probiotic Escherichia coli Nissle 1917 strain (Mutaflor® suspension, Ardeypharm GmbH, Herdecke, Germany) on bacterial translocation in cases of liver damage, damage to the intestinal mucosa, potential portal hypertension associated with possible development of oesophageal varices and on the bacterial population of the intestine during chronic experimental liver damage in the laboratory rat. Rats with liver damage induced by thioacetamide were divided into an experimental and control group. Experimental and control animals were applied Mutaflor and saline, respectively. Samples of blood,...