Veterinární medicína, 2010 (vol. 55), issue 4
Effects of Border disease virus (genotype 3) naturally transmitted by persistently infected sheep to pregnant heifers and their progeny
R. Krametter-Froetscher, N. Mason, J. Roetzel, V. Benetka, Z. Bago, K. Moestl
Vet Med - Czech, 2010, 55(4):145-153 | DOI: 10.17221/29/2010-VETMED
Eight heifers pregnant between days 47 and 73 were kept together with nine healthy persistently Border disease virus (BDV)-infected sheep allowing natural contact comparable to field conditions. All heifers seroconverted between days 23 and 38 after exposure. Besides a mild increase in body temperature in four heifers, no clinical signs of infection were observed, but 5 animals aborted between days 54 and day 202 after exposure. BDV was detected in the aborted fetuses of four heifers and in the placenta of the fifth (the only material available). Foetal mummification was seen in three foetuses, aborted between days 113 and 116 of gestation, with a...
The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2 protein levels--
P. Antosik, B. Kempisty, M. Jackowska, D. Bukowska, M. Lianeri, K.P. Brussow, M. Wozna, J.M. Jaskowski
Vet Med - Czech, 2010, 55(4):154-162 | DOI: 10.17221/38/2010-VETMED
The morphology and quality of oocytes is set during oo- and folliculo-genesis, and is completed during final maturation. Early embryonic development is associated with the morphology of the cumulus-oocyte-complex (COC). However, current knowledge of the possible relationships between oocyte morphology and the level of proteins within the oocyte, which may reflect fertilization ability, is insufficient. Using western-blot analysis and confocal microscopic observation, we determined the levels of integrin beta-2 (integrin β2) and glycoprotein 3 (pZP3) protein levels in the porcine zona pellucida of four morphologically different types of...
Malondialdehyde levels in serum of dogs infected with Babesia canis
M. Crnogaj, R. Petlevski, V. Mrljak, I. Kis, M. Torti, N. Kucer, V. Matijatko, I. Sacer, I. Stokovic
Vet Med - Czech, 2010, 55(4):163-171 | DOI: 10.17221/77/2010-VETMED
Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory...
Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections
S.-J. Du, H.-C. Kuo, C.-H. Cheng, A.C.Y. Fei, H.-W. Wei, S.-K. Chang
Vet Med - Czech, 2010, 55(4):172-182 | DOI: 10.17221/64/2010-VETMED
Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 2008. The minimal inhibitory concentrations (MICs) of the antimicrobial agents of these isolates were determined. Multidrug resistance was common, with 23 (34.8%) isolates found to be ceftazidime resistant. To explore the mechanisms of ceftazidime resistance, PCR analyses were performed to detect drug-resistance genes. The prevalence rate of Ambler class A, B, and D β-lactamase genes were obtained, with blaTEM-1 100%, blaPSE-1...
The spinal nerves that constitute the plexus lumbosacrales of the red squirrel (Sciurus vulgaris)
A. Aydin
Vet Med - Czech, 2010, 55(4):183-186 | DOI: 10.17221/3021-VETMED
This study was aimed at investigating the lumbosacral plexus of squirrels (Sciurus vulgaris). Ten squirrels were used in this work. The animals were carefully dissected and the spinal nerves that constitute the lumbosacral plexus were examined. It was discovered that the lumbosacral plexus was formed by L6, L7, S1, S2 in nine animals and in the one remaining animal in addition to these branches rami ventralis of L4 and L5 were forming the plexus. The common root forming the last part of the lumbosacral plexus and giving branches dispersing to the hind limb was formed by the rami ventralis of L6, L7, S1, S2. Thus, in squirrels, the spinal nerves...
Equine cyathostomosis: case reports
S. Bodecek, P. Jahn, O. Dobesova, E. Vavrouchova
Vet Med - Czech, 2010, 55(4):187-193 | DOI: 10.17221/88/2010-VETMED
Twelve clinical cases of cyathostomosis in horses treated at the Equine Clinic University of Veterinary and Pharmaceutical Sciences in Brno, the Czech Republic, between the years 1999 and 2008 are described in this report. Six cases (50%) were hospitalized in the period from 2007 to 2008. Eleven of them were hospitalized in the period from December to March. Only one case was admitted in June, but the clinical signs had appeared for the first time in January. All horses described in these cases were younger than six years of age. Diarrhoea as a predominant clinical sign was present in four horses and colic in four horses. One horse showed both colic...
Multiple congenital malformation in a Simental female calf: a case report
O. Smolec, J. Kos, D. Vnuk, M. Stejskal, N. Brkljaca Bottegaro, R. Zobel
Vet Med - Czech, 2010, 55(4):194-198 | DOI: 10.17221/95/2010-VETMED
Congenital anomalies in offspring of natural breedings are often a result of environmental factors, genetic factors, or both. A 21-day-old male Simental female calf was submitted to our clinic with abnormal severe green mucoid nasal discharge from both nostrils. At necropsy, the calf showed multiple defects, including cleft palate, hydrocephalus, diphragmatic hernia and freemartinism. The cause of these anomalies could not be determined. This report is the first to describe these multiple malformations in a single calf.