Veterinární medicína, 2012 (vol. 57), issue 3
Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic
E. Bartova, K. Sedlak
Vet Med - Czech, 2012, 57(3):111-114 | DOI: 10.17221/5850-VETMED 
Toxoplasma gondii is zoonotic protozoan parasite that causes infections in many vertebrate species. The present study determined the seroprevalence of T. gondii and N. caninum in goats from the Czech Republic. Serum samples were collected from 251 healthy adult goats in the Czech Republic during the years 2006 to 2009. Sera samples were tested for serum antibodies to Toxoplasma gondii by an enzyme-linked immunosorbent assay with cut off equal to or higher than 50% S/P. The same samples were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay with...
The prevalence of hepatitis E virus in piglets on Czech pig production farms and phylogenetic analysis of recovered isolates
E. Kosinova, J. Bendova, P. Vasickova, R. Smitalova, J. Prodelalova
Vet Med - Czech, 2012, 57(3):115-120 | DOI: 10.17221/5858-VETMED
The aim of our study was to determine the prevalence of hepatitis E virus (HEV) in domestic pigs and to investigate the genetic divergence of swine HEV in the Czech Republic. To this end, a one-step real time RT-PCR assay was introduced as a screening method while nested RT-PCR was used as an additional method to obtain specific sequences from the HEV genome and thus to perform sequence analysis. A total of 63 piglets originating from 14 farms were examined. Bile and intestinal contents were collected from each animal. At least one HEV RNA positive piglet was found in ten (71.4%) of the monitored farms. HEV RNA was most frequently detected in bile...
Comparative single intraperitoneal dose pharmacokinetics of aspirin and acetaminophen in chicks
F.K. Mohammad, A.S. Mansoor, M.H.I. Al-Zubaidy
Vet Med - Czech, 2012, 57(3):121-124 | DOI: 10.17221/5851-VETMED
Limited information is available on the pharmacokinetics and bioavailability of aspirin and acetaminophen in young chicks. The purpose of the present study was to examine the pharmacokinetics of acetyl salicylic acid (aspirin) and acetaminophen in 12-day old chicks after a single intraperitoneal administration of each drug alone at the dose of 100 mg/kg body weight. Blood samples were collected from chicks (six/each time period) at 10, 20, 30, 60 and 120 min after each drug administration. The concentrations of aspirin and acetaminophen in the plasma were determined by spectrophotometric methods. The pharmacokinetic parameters of the drugs were calculated...
Prevalence of etiological agents of selected respiratory infections in chicken and turkey farms in the Czech Republic
D. Lobova, L. Kohoutova, D. Molinkova, K. Rosenbergova, O. Kubicek, V. Celer
Vet Med - Czech, 2012, 57(3):125-132 | DOI: 10.17221/5852-VETMED
The causative agents of respiratory diseases of turkeys represent, primarily in fattening farms, a substantial risk of economic and breeding problems. The purpose of this communication is to provide information on the prevalence of respiratory agents of turkeys and chickens in several fattening and production farms in Southern Moravia. This study was focused on pathogens causing bacterial diseases such as Ornithobacteriosis and Mycoplasmosis, as well as viral rhinotracheitis and laryngotracheitis of poultry. The laboratory diagnosis of these diseases has been performed in our institute since January 2008. We examined 249 samples of turkeys...
DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa
P. Prinosilova, R. Rybar, A. Zajicova, J. Hlavicova
Vet Med - Czech, 2012, 57(3):133-142 | DOI: 10.17221/5853-VETMED
Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). In the first experiment, the chromatin integrity of fresh semen from 60 dogs with differing histories of fertility was compared with other sperm parameters (total sperm count, sperm motility, viability, acrosomal integrity and sperm morphology). Except for 15 dogs that had never mated before, all were used in breeding as semen donors. Thirty-three of them were successful breeding males while in 12 repeated fertility problems were noted. Ejaculates were...
Descriptive study of botulism in an Austrian dairy herd: a case report
J. Kummel, R. Krametter-Froetscher, G. Six, R. Brunthaler, W. Baumgartner, B. Altenbrunner-Martinek
Vet Med - Czech, 2012, 57(3):143-149 | DOI: 10.17221/5854-VETMED
An outbreak of botulism was suspected at an Austrian dairy farm in June 2010. Six Simmental cows, out of a herd of 29, were affected and showed the typical signs. The affected cows included either animals suffering from sudden recumbency and reduced tongue tone or others which developed paresis, which gradually led to recumbency. Most of the affected animals died. Two cases were submitted to the Clinic for Ruminants at the University of Veterinary Medicine Vienna in order to clarify the cause of illness. The animals had reportedly been fed recently with silage possibly polluted by discarded cat carcasses spread onto the pasture used for the silage...
Lymphoma of the trachea in a cat: a case report
F. Jelinek, F. Hozmanova
Vet Med - Czech, 2012, 57(3):150-153 | DOI: 10.17221/5855-VETMED
Diffuse large B-cell lymphoma of the trachea was diagnosed in a 14-year-old domestic shorthaired cat suffering from dyspnoea. X-ray examination revealed marked stenosis of the lumen in the thoracic segment of the trachea. Post-mortem examination of the trachea disclosed two formations. The larger, visible in the radiograph, was formed by thickened tracheal wall. The smaller one was of crest appearance and was located in the cranial segment of the trachea, immediately behind the larynx. The tumour was characterised by immunohistochemical positivity to CD79αcy epitope in the neoplastic cells.
Diagnosis of a Dicrocoelium dendriticum infection in New World Camelids: a case report
D. Klein, H. Prosl, D. Thaller, M. Floeck
Vet Med - Czech, 2012, 57(3):154-162 | DOI: 10.17221/5856-VETMED
Dicrocoelium dendriticum plays an important role in New World Camelids as infected animals may suffer from severe clinical symptoms even leading to death of the animals. Intra vitam diagnosis may be difficult as clinical signs are atypical and Dicrocoelium eggs are shed only intermittently in faeces. The aim of this paper is to present four clinical cases of dicrocoeliosis in lamas as well as three asymptomatic infected animals to support the veterinarian in practice to diagnose infections. Furthermore, it is the first time that ultrasonographic examinations are described in this context. All seven lamas had been admitted to the Clinic...
Infection in a female Komodo dragon (Varanus komodoensis) caused by Mycobacterium intracellulare: a case report
M. Skoric, V. Mrlik, J. Svobodova, V. Beran, M. Slany, P. Fictum, J. Pokorny, I. Pavlik
Vet Med - Czech, 2012, 57(3):163-168 | DOI: 10.17221/5857-VETMED
In early 2002, a bean-like whitish nodule 2 × 1 × 1 cm in size was diagnosed on the tongue of a female Komodo dragon (Varanus komodoensis) kept in a zoological garden in the Czech Republic. The nodule was removed at surgery and histopathological examination revealed a specific granulomatous inflammation. The granuloma contained a necrotic mass surrounded by a variable layer of palisading epithelioid macrophages and multinucleated giant cells with a variable admixture of lymphocytes and plasma cells. Signs of mineralisation within the granuloma were not observed. Using Ziehl-Neelsen (ZN) staining the presence of acid-fast rods (AFR) was determined...