Veterinární medicína, 2012 (vol. 57), issue 5
Direct and indirect assessment of selenium status in sheep - a comparison
L. Pavlata, L. Misurova, A. Pechova, T. Husakova, R. Dvorak
Vet Med - Czech, 2012, 57(5):219-223 | DOI: 10.17221/5951-VETMED 
The aim of this study was to determine the relationship between selenium concentration and activity of glutathione peroxidase (GSH-Px) in the whole blood of sheep and reference ranges for the activity of GSH-Px for evaluation of the selenium status of sheep in the Czech Republic. Selenium concentration and activity of glutathione peroxidase were determined in whole blood samples collected from 92 sheep in six herds. The GSH-Px activity in the samples was measured using the photometric method. Selenium in the sample was measured using the hydride technique atomic absorption spectrometry. Data on both parameters were processed using correlation and regression...
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
M. Adamska, A. Leonska-Duniec, M. Sawczuk, A. Maciejewska, B. Skotarczak
Vet Med - Czech, 2012, 57(5):224-232 | DOI: 10.17221/5952-VETMED
Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally robust nature of the oocyst's walls, which necessitates more stringent treatments for disruption and recovery of DNA for analysis using molecular methods. In the case of water, which is the major source of Cryptosporidium oocysts, investigations concern the detection of the presence of the oocysts. Their concentration in water is very low, and moreover, many substances that may have significance as inhibitors of DNA amplification, are...
Reference data of clinical chemistry, haematology and blood coagulation parameters in juvenile cynomolgus monkeys (Macaca fascicularis)
H. Wang, Y.Y. Niu, W. Si, Y.J. Li, Y. Yan
Vet Med - Czech, 2012, 57(5):233-238 | DOI: 10.17221/5953-VETMED
Juvenile cynomolgus monkeys are valuable models for studying human diseases. Reference data of clinical chemistry, haematology and blood coagulation parameters of juvenile cynomolgus monkeys are very important for clinical diagnosis and conducting research. In this study, 72 blood samples (obtained from 35 males and 37 females) and 20 blood samples (obtained from 10 males and 10 females) were used to determine normal data of clinical serum chemistry, haematological profiles and normal blood coagulation parameters in juvenile cynomolgus monkeys. Seventeen markers of clinical serum chemistry, twenty-nine markers of haematology and two parameters of blood...
The branching of the aortic arch in the Eurasian bittern (Botaurus stellaris, Linnaeus 1758)
S. Erdogan
Vet Med - Czech, 2012, 57(5):239-244 | DOI: 10.17221/5954-VETMED
This study was aimed at determining the vascular architecture of the aortic arch in the Eurasian bittern. For this purpose, the heart arteries of two bitterns were evaluated. The latex injection method was used to observe the branching of the aortic arch. Two brachiocephalic trunks were arising separately from the aortic arch and these arteries were giving to the common carotid and subclavian arteries. One of the thin branches arising from the subclavian artery was the sternoclavicular artery, which was in turn dividing into a sternal and a clavicular artery supplying the thoracic inlet and pectoral muscles. After the branching of the sternoclavicular...
Evaluation of the developmental toxicity of 2-phenoxyethanol and clove oil anaesthetics using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX)
D. Vrskova, H. Modra
Vet Med - Czech, 2012, 57(5):245-250 | DOI: 10.17221/5955-VETMED
The developmental toxicity of two anaesthetics, 2-phenoxyethanol and clove oil, used in aquaculture was evaluated using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) and the results were compared to outcomes in fish. Xenopus laevis embryos were exposed to 50, 100, 300, 500, 700 and 1000 mg/l of 2-phenoxyethanol or 1, 5, 10, 20, 30 and 40 mg/l of clove oil. Values of 96 h LC50, 96 h EC50 (malformation) and teratogenic index (ratio of 96 h LC50 and 96 h EC50) were determined and the types and severities of the induced malformations and minimal concentration inhibiting the growth of embryos were estimated. Teratogenic index values...
Immunohistochemical profile of multicentric cutaneous epitheliotropic T-cell lymphoma with generalised lymphadenopathy in a Holstein-Friesian cow: a case report
I. Otrocka-Domagala, Z. Procajlo, K. Pazdzior, M. Gesek, T. Rotkiewicz, W. Szweda
Vet Med - Czech, 2012, 57(5):251-257 | DOI: 10.17221/5956-VETMED
Cutaneous epitheliotropic T-cell lymphoma with progression to superficial and internal lymph nodes and the spleen was diagnosed in a two-year-old Holstein-Friesian cow. The skin lesions included multiple hypotrichous to alopecic nodules, often with ulceration, which first appeared three months after calving and progressed quickly to cover the entire body. The cow was euthanized one month later. The ELISA test excluded bovine leukaemia virus infection. Histologically in the epidermis, the formation of Pautrier's microabscesses and in the dermis, dense neoplastic infiltration with mild folliculotropism was observed. Similar neoplastic cells were present...
Treatment of an infected total knee replacement with two-stage arthrodesis in a dog: a case report
I.S. Kim, C.S. Kim, K.C. Lee, N.S. Kim, M.S. Kim, M.R. Alam, H.B. Lee
Vet Med - Czech, 2012, 57(5):258-262 | DOI: 10.17221/5957-VETMED
A three-year-old intact female Great Pyrenees underwent total knee replacement for the treatment of osteoarthritis. The dog contracted septic arthritis 10 weeks following the procedure. Treatments including implant subtraction, debridement of tissue, and insertion of bone cement with antibiotics into the joint space were completed in the joint cavity. A temporary external skeletal fixator was used to stabilise the joint for four weeks. A second attempt at total knee replacement failed due to severe muscle contracture and bone loss. Therefore, it was decided to perform arthrodesis as a salvage procedure. At the 17 month follow-up examination, the patient...
Hemangiopericytoma in a cat: a case report
A. Blutke, J. Knebel, A. Brühschwein, W. Breuer, W. Hermanns
Vet Med - Czech, 2012, 57(5):263-269 | DOI: 10.17221/5958-VETMED
In dogs and men, hemangiopericytoma is a well-recognised neoplasm, thought to originate from vascular pericytes. In cats, however, hemangiopericytoma is an extremely rare finding. The present report describes the pathological features of a 7-cm-diameter, dense, white, multilobulated tumour-like growth on the thigh of a ten-year-old Persian cat. Histologically, the mass consisted of polygonal neoplastic cells, concentrically arranged around thin-walled endothelium-lined blood vessels occasionally forming typical staghorn-configurations. In reticulin-stained sections, a dense meshwork of argyrophilic collagen fibres was evident, surrounding the central...
Magnetic resonance imaging of a sacral bone with telangiectatic osteosarcoma in a dog: a case report
Z. Adamiak, A. Pomianowski, I. Otrocka-Domagala, M. Jaskolska
Vet Med - Czech, 2012, 57(5):270-273 | DOI: 10.17221/5959-VETMED
A canine patient with osteosarcoma of the sacrum was subjected to low-field magnetic resonance imaging with the following sequences: FSE, SE, 3D HYCE, X BONE, Fast STIR, GE STIR and Gradient Echo. Due to the size and location of the tumour, surgery was not performed, and the patient was euthanized. A histopathological analysis of the tumour revealed telangiectatic osteosarcoma.