Veterinární medicína, 2013 (vol. 58), issue 10
The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a reviewReview
H. Piotrowska, B. Kempisty, P. Sosinska, S. Ciesiolka, D. Bukowska, P. Antosik, M. Rybska, K.P. Brussow, M. Nowicki, M. Zabel
Vet Med - Czech, 2013, 58(10):505-515 | DOI: 10.17221/7082-VETMED 
The normal differentiation of follicles from the preantral to the antral stage is regulated by the synthesis and secretion of several important growth factors. Moreover, the proper growth and development of the oocyte and its surrounding somatic granulosa-cumulus cells is accomplished through the activation of paracrine pathways that form a specific cross-talk between the gamete and somatic cells. It has been shown that several growth factors produced by the ovary are responsible for the proper growth and development of follicles. The developmental competence of mammalian oocytes (also termed developmental potency) is defined as the ability of female...
A review on Schmallenberg virus infection: a newly emerging disease of cattle, sheep and goatsReview
R.V.S. Pawaiya, V.K. Gupta
Vet Med - Czech, 2013, 58(10):516-526 | DOI: 10.17221/7083-VETMED
Schmallenberg virus (SBV) infection is an emerging infectious disease of ruminants first described in Germany in November, 2011. Since then it has spread very rapidly to several European countries. The disease is characterised by fever, reduced milk production and diarrhoea in cattle and abortions, stillbirths and foetal abnormalities in sheep and goats. SBV is an enveloped, negative-sense, segmented, single-stranded RNA virus, classified in the genus Orthobunyavirus of the Bunyaviridae family, and is closely related to Akabane, Ainoa and Shamonda viruses. As of now there is no vaccine available for SBV, which poses a serious threat to naive...
A new device for the aspiration of follicular fluid for acid-base balance analysis in cattleOriginal Paper
S. Cech, R. Dolezel, K. Holickova, E. Indrova, T. Kochova, M. Lopatarova, J. Mala, A. Pechova, M. Zavadilova, L. Zilka
Vet Med - Czech, 2013, 58(10):527-534 | DOI: 10.17221/7084-VETMED
The aim of this study was to evaluate a new device for the ultrasound-guided transvaginal aspiration of follicular fluid for acid-base balance analysis (ABB set) in comparison with the original modified commercial OPU set. In the ABB set, an aspiration syringe was placed in the front part of the new tool's handle, next to the transducer, so as to enable direct collection of the sample into the syringe. To obtain a sufficient amount of testable fluid, reservoirs of urine (rubber balloons) were used for later aspiration under laboratory conditions in Experiment 1. Fifteen triads of samples (each triad with two punctures) were collected. While the first...
Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of CryptosporidiumOriginal Paper
M.C.M. Couto, A.P. Sudre, M.F. Lima, T.C.B. Bomfim
Vet Med - Czech, 2013, 58(10):535-542 | DOI: 10.17221/7085-VETMED
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. However, if these tools are to be used correctly and accurately, the techniques used must be standardised. In the present study, two molecular techniques for diagnosing Cryptosporidium infection in cows were compared to determine the optimal methods. For each technique, we tested two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA),...
Thickness of the substantia compacta of porcine long bonesOriginal Paper
M. Pyszko, V. Paral, M. Kyllar
Vet Med - Czech, 2013, 58(10):543-552 | DOI: 10.17221/7086-VETMED
Mechanical load on the bone influences bone tissue and its inner tension, subsequently affecting bone formation and its histological structure. A precise understanding of this load and the development of strategies to influence it would contribute to principles of fracture management and to solving other bone pathologies of both humans and animals. The long bones of the thoracic and pelvic limb of a pig were here used as possible models to test new devices and implant materials. The purpose of this study was to investigate the thickness of compact bone of the diaphysis of porcine long bones and to establish ideal insertion points for tensometer probes,...
Secondary poisoning of non-target animals in an Ornithological Zoo in Galicia (NW Spain) with anticoagulant rodenticides: a case reportCase Report
D. Hernandez-Moreno, I. de la Casa-Resino, A. Lopez-Beceiro, L.E. Fidalgo, F. Soler, M. Perez-Lopez
Vet Med - Czech, 2013, 58(10):553-559 | DOI: 10.17221/7087-VETMED
The use of anticoagulants has increased in recent times as a method for controlling rodent populations. However, this increased use also provokes accidental and intentional ingestion for both animals and humans, triggering poisoning of non-target organisms. In the present report, a clinical case of secondary-poisoning of birds with anticoagulant rodenticides, which took place after a general rodenticide treatment in an Ornithological Zoological Park, is described. Three birds died as a result and samples were submitted to the Veterinary Hospital in Lugo (Galicia, NW Spain). After necropsy, samples of the birds, together with molluscs and faeces, were...