Veterinární medicína, 2014 (vol. 59), issue 2
Reliability of ultrasonographic examination of the large intestine in healthy cowsOriginal Paper
S. Imran, S.P. Tyagi
Vet Med - Czech, 2014, 59(2):63-67 | DOI: 10.17221/7314-VETMED 
The aim of this study was to assess the usefulness of ultrasonographic examination of the large intestine in 10 clinically healthy Jersey/Red Sindhi crossbred cows. The area extending from the tuber coxae to the 6th intercostal space (ICS) and from the lumbar transverse processes to the linea alba on the right side was shaved. An imaginary line was drawn from the distal third of the femur up to the 8th ICS parallel to the longitudinal axis of the cow. The large intestine was scanned dorsal to this imaginary line. Only the near wall of the large intestine adjacent to the abdominal wall could be imaged ultrasonographically. Based...
Changes of amino acid concentrations in Polish Merino sheep between 21 and 150 days of lifeOriginal Paper
M.R. Tatara, A. Brodzki, K. Pasternak, M. Szpetnar, P. Rosenbeiger, B. Tymczyna, D. Niedziela, W. Krupski
Vet Med - Czech, 2014, 59(2):68-75 | DOI: 10.17221/7315-VETMED
The aim of this study was to evaluate plasma amino acid profiles and their interrelationships in male Polish Merino sheep at two different stages of their systemic development - at the neonatal monogastric stage and as a fully grown ruminant. Male lambs (n = 12, singles) were kept indoors in pens under standard rearing conditions and weaned at the age of 10 weeks of life. Blood samples were collected on day 21 and 150 of life. Free amino acids were determined in plasma using ion-exchange chromatography. There was a significant reduction in plasma amino acid concentrations between neonatal and functioning ruminants for threonine, serine, glutamate,...
Aberrant cytoplasmic accumulation of retinoblastoma protein in basal cells may lead to increased survival in malignant canine mammary tumoursOriginal Paper
S. Gautam, N.K. Sood, K. Gupta
Vet Med - Czech, 2014, 59(2):76-80 | DOI: 10.17221/7316-VETMED
The retinoblastoma susceptibility gene RB-1 is a tumour suppressor gene that encodes a protein (Rb) that regulates the transition from the G1 phase to the S phase of the cell cycle. Inactivation of the Rb gene has been shown in a variety of human tumours, including breast, ovarian, hepatic, prostatic, and endometrial carcinomas. Although Rb protein is normally expressed in the nuclei of healthy cells, during carcinogenesis there is a partial or complete loss of nuclear expression. Recently, some reports have indicated aberrant cytoplasmic expression of Rb protein. However, little is known about its cytoplasmic expression and significance as...
The effects of four anaesthetics on haematological and blood biochemical profiles in vimba bream, Vimba vimbaOriginal Paper
P. Lepic, A. Stara, J. Turek, P. Kozak, J. Velisek
Vet Med - Czech, 2014, 59(2):81-87 | DOI: 10.17221/7317-VETMED
The aim of this study was to compare the effect of four anaesthetics on the haematological and blood biochemical profiles of vimba bream (Vimba vimba). The haematological and blood biochemical profiles of vimba bream were evaluated 10 min and 24 h after anaesthesia with MS 222 (100 mg/l), clove oil (33 mg/l), 2-phenoxyethanol (0.4 ml/l), Propiscin (1.0 ml/l) and compared to non-anaesthetised controls. The 10 min exposure to any of the anaesthetics did not show any effects on haematological profiles. The exposure to 2-phenoxyethanol and Propiscin significantly (P < 0.01) influenced levels of glucose and ammonia, and the activity...
The effect of the cannabinoid CB1 receptor agonist arachidonylcyclopropylamide (ACPA) on behavioural sensitisation to methamphetamine in miceOriginal Paper
L. Landa, K. Slais, A. Machalova, A. Sulcova
Vet Med - Czech, 2014, 59(2):88-94 | DOI: 10.17221/7318-VETMED
The psychostimulant methamphetamine (Met), similarly to other drugs of abuse, is known to produce an increased behavioural response after its repeated application (behavioural sensitisation). It has also been described that an increased response to a drug may be elicited by previous repeated administration of another drug (cross-sensitisation). We have previously shown that the CB1, CB2 and TRPV (vanilloid) cannabinoid receptor agonist methanandamide, cross-sensitised to Met stimulatory effects in mice. The present study was focused on ability of the more selective and potent CB1 receptor activator arachidonylcyclopropylamide...
Homozygous Pelger-Huet anomaly in three different crossbred rabbits: a case reportCase Report
P. Supuka, D. Mazensky, A. Supukova, V. Parkanyi, L. Ondruska, S. Hornak, R. Hromada, V. Petrovic
Vet Med - Czech, 2014, 59(2):95-101 | DOI: 10.17221/7319-VETMED
In this case report, three different crossbreedings of pet rabbits were performed producing affected as well as healthy bunnies. All affected rabbits were smaller and had local alopecia, exophthalmus, and limb deviations compared to their healthy littermates; thus, a homozygous form of Pelger-Huet anomaly was suspected. This anomaly was confirmed by blood examination in which granulocytes with oval nuclei and a very coarse chromatin pattern, as well as lymphocytes with micronuclei were noticed. Karyotype analyses of the lymphocytes revealed many chromosomal aberrations in affected rabbits. Moreover, severe arterial abnormalities in the pelvic cavity...
Uterine and ovarian remnants in an incorrectly spayed bitch: a case reportCase Report
C.C. Perez-Marin, L. Molina, G. Vizuete, J.M. Sanchez, R. Zafra, M.J. Bautista
Vet Med - Czech, 2014, 59(2):102-106 | DOI: 10.17221/7320-VETMED
A spayed Samoyed bitch, 12 years old, was presented to the Veterinary Clinical Hospital of the University of Cordoba (Spain) with abundant vulvar sanguineous discharge over the previous three days. The clinical examination revealed a remarkable vulvar mass, which protruded through the vulvar lips. Abdominal ultrasonography revealed the presence of structures compatible with uterus and ovary, which had been presumably removed eight years previously. An exploratory laparotomy was carried out, which confirmed the presence of the right ovary and a remnant of the uterus. The histological evaluation confirmed a granulosa cell tumour in the ovary, and an...
Prepubic urethrostomy opening within a prepuce in a dog: a case reportCase Report
D. Vnuk, N. Brkljaca Bottegaro, L. Slunjski, B. Skrlin, A. Musulin, M. Stejskal
Vet Med - Czech, 2014, 59(2):107-111 | DOI: 10.17221/7321-VETMED
The prepubic urethrostomy procedure has been shown to be a long-term diversion technique in dogs with intrapelvic urethral damage. This case report describes the clinical presentation and successful surgical management of an intrapelvic urethral stricture in a four month old male intact Labrador Retriever with hypospadias. The dog was presented for assessment of dysuria, stranguria, urinary incontinence, and urethrocutaneous fistula. After the diagnosis of perineal hypospadias with intrapelvic urethral stricture was made, surgery was performed to relieve the urethral obstruction and repair the urethrocutaneous fistula. A prepubic urethrostomy was made...
Sodium carbonate intoxication on a chinchilla (Chinchilla lanigera) farm: a case reportCase Report
J. Wojtacka, J. Szarek, I. Babinska, M. Felsmann, E. Strzyzewska, A. Szarek-Beska, K. Dublan, J. Micinski
Vet Med - Czech, 2014, 59(2):112-116 | DOI: 10.17221/7322-VETMED
Massive deaths were reported on a chinchilla (Chinchilla lanigera) farm, which over 10 months led to the elimination of the herd. After three months of feeding, longitudinal precipitates inside the pellets were noted. The first symptoms were observed two weeks after the introduction of feed, and included lethargy, decreased mobility, and reduced appetite, as well as increased reactions to external stimuli. Over time, single deaths, hair loss and gnawing, white discolorations on teeth, and polyuria were reported. Haemorrhages of the reproductive tract and mass abortions were observed. Necropsy revealed the presence of transudate with pH = 10,...