Veterinární medicína, 2016 (vol. 61), issue 1

Polymorphism and expression of the tumour necrosis factor-alpha (TNF-alpha) gene in non-infected cows and in cows naturally infected with the bovine leukaemia virus (BLV)Original Paper

B. Bojarojc-Nosowicz, P. Brym, E. Kaczmarczyk, A. Stachura, A.K. Habel

Vet Med - Czech, 2016, 61(1):1-9 | DOI: 10.17221/8676-VETMED  

The objective of this study was to determine the influence of TNF-alpha gene polymorphism at position -824 A > G on TNF-alpha gene expression in BLV-positive animals with aleukaemic (AL) and persistent lymphocytosis (PL) forms of enzootic bovine leukosis (EBL) and in BLV-negative cows. Polymorphism of the TNF-alpha gene at position -824 A > G had a complex influence on TNF-alpha gene expression in cows infected with BLV. In animals with various TNF-alpha genotypes, mRNA levels were stable and similar, but significant differences were noted in the percentages of PBMCs expressing membrane TNF-alpha (mTNF-alpha) protein (PBMCs that were positive...

Protective effects of the antioxidants curcumin, ellagic acid and methionine on motility, mitochondrial transmembrane potential, plasma membrane and acrosome integrity in freeze-thawed Merino ram spermOriginal Paper

A.D. Omur, K. Coyan

Vet Med - Czech, 2016, 61(1):10-16 | DOI: 10.17221/8677-VETMED  

The aim of this study was to determine the effects of curcumin, ellagic acid and methionine on sperm parameters following the freeze-thawing of Merino ram semen. Ejaculates were collected via an artificial vagina from four Merino rams, evaluated microscopically and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender and separated into groups containing curcumin (1, 2, 4mM), ellagic acid (1, 2, 4mM), methionine (1, 2, 4mM) and no antioxidant (control). The diluted semen was cooled to 5 °C slowly and equilibrated for 3 h. After the equilibration, the samples were frozen in liquid nitrogen vapour, and plunged into liquid nitrogen...

Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of TurkeyOriginal Paper

A. Gulmez Saglam, M. Sahin

Vet Med - Czech, 2016, 61(1):17-22 | DOI: 10.17221/8678-VETMED  

This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these examinations revealed that 108 (43.2%) out of 250 sheep blood serum samples and 52 (14.85%) out of 350 cattle blood serum samples were seropositive for C. burnetii antibodies by ELISA. Out of 350 cattle and 250 sheep milk samples examined with ELISA, 36 (10.28%) and 42 (16.8%) were found to be seropositive, respectively. For molecular analysis,...

Maternal immunity induced by inactivated S. Typhimurium vaccine is less protective to S. Derby challenge than to S. Typhimurium challenge in suckling pigletsOriginal Paper

J. Gebauer, A. Osvaldova, H. Kudlackova, M. Maceckova, F. Sisak, H. Havlickova, P. Ondrackova, L. Leva, M. Faldyna, J. Matiasovic

Vet Med - Czech, 2016, 61(1):23-27 | DOI: 10.17221/8679-VETMED  

Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Derby are the most common serovars of Salmonella enterica ssp. enterica found in pigs in Europe. We previously observed that suckling piglets of sows vaccinated with an S. Typhimurium-based inactivated vaccine are protected against homologous strain challenge. To develop this vaccine for commercial use, potential crossprotectivity of this vaccine to challenge with S. Derby was tested. Two sows were vaccinated with an S. Typhimurium-based inactivated vaccine while two other sows remained serologically negative....

Effects of dietary Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile in broilersOriginal Paper

M. Mohammadi Gheisar, A. Hosseindoust, I.H. Kim

Vet Med - Czech, 2016, 61(1):28-34 | DOI: 10.17221/8680-VETMED  

This research was performed to evaluate the effect of supplementing broiler diets with a probiotic containing Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile. A total of 384 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 39.2 g were used in a 35 days feeding trial. The chicks were allotted to pens with 16 birds per pen and eight replications per treatment with food and water provided ad libitum. Treatments were: (1) basal diet, (2) 0.25% probiotic, and (3) 0.5% probiotic. Results indicated that body weight gain (BWG) on Day 7 to 21, Day 21...

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copperOriginal Paper

M. Sevcikova, H. Modra, J. Blahova, R. Dobsikova, L. Plhalova, O. Zitka, D. Hynek, R. Kizek, M. Skoric, Z. Svobodova

Vet Med - Czech, 2016, 61(1):35-50 | DOI: 10.17221/8681-VETMED  

The aim of the present study was to investigate the impact of copper-based pesticides (at concentrations of copper of 20, 30, 40 and 70 µg/l) on one-year-old common carp (Cyprinus carpio L.) during 28 days of exposure. Abnormal behaviour was observed in fish exposed to 70 µg/lfrom Day14. Histological alterations were noticed only in liver in the groups exposed to 40 and 70 µg/l.Significant changes (P ˂ 0.05) in almost all haematological indices were found, especially in the group exposed to the highest concentration of copper (70 µg/l). Biochemical analysis revealed various significant (P ˂...

Secondary abdominal pregnancy with foetal mummification diagnosed using computed tomography in a dog: a case reportCase Report

H.W. Myung, A.J. Lee, J.Y. Kim, J.H. Kim, K.D. Eom, H.J. Kim, S.H. Do, H.Y. Kim, D.J. Chung

Vet Med - Czech, 2016, 61(1):51-55 | DOI: 10.17221/8682-VETMED  

An intact female Maltese dog, rescued two months previously, and weighing 2.7 kg, was presented for ovariohysterectomy. There were no typical clinical signs and evidences of parturitions. A firm abdominal mass was palpated, which was identified as a round-shaped radiopaque mass in survey radiography. The mass was tentatively diagnosed as an extrauterine foetus with mummification on computed tomography (CT). Surgical removal of the mass and ovariohysterectomy were performed. Histopathological examination yielded a final diagnosis of abdominal ectopic pregnancy