Vet Med - Czech, 2025, 70(11):417-426 | DOI: 10.17221/41/2025-VETMED

Experimental ultrasound stimulator for improving the diffusion of exosomes and drugs into lung tissueOriginal Paper

J Prucha1, J Skopalik1, T Parak2,3, P Bratka1, J Cuprova4
1 Department of Communication and Information Technologies, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic
2 Laboratory Medicine Centre, Trauma Hospital of Brno, Brno, Czech Republic
3 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic
4 Department of Healthcare and Population Protection, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic

Pulmonary fibrosis is not only a consequence of the recent COVID-19 pandemic, but is increasingly recognised by both human and veterinary healthcare providers. Pulmonary fibrosis is a progressive condition that leads to a decline in respiratory function and even death. In this work, we compared MSC-derived exosomes with conventional anti-inflammatory drug treatments. Exosomes from stimulated MSCs displayed higher miRNA concentrations (in particular, miRNA-30b was significantly increased). A set of rats with induced lung fibrosis were divided into four groups: NC (control – no treatment), A2 (exosome infusion), A3 (exosome infusion combined with ultrasound stimulation), and F1 (tamoxifen/metformin drug treatment). The rats’ lungs were subjected to histological analysis; the fibrosis scores for groups F1 and A3 were very similar and decreased significantly compared with group NC. Ultrasound-facilitated diffusion of exosomes from the capillaries into the lung tissue could represent an innovative therapeutic approach for slowing fibrosis and prolonging the active life of the organism.

Keywords: drug infusion; electromagnetic stimulus; exosomes; fibrosis; therapeutic ultrasound

Received: May 28, 2025; Revised: August 23, 2025; Accepted: August 25, 2025; Prepublished online: November 27, 2025; Published: November 28, 2025  Show citation

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Prucha J, Skopalik J, Parak T, Bratka P, Cuprova J. Experimental ultrasound stimulator for improving the diffusion of exosomes and drugs into lung tissue. Vet Med - Czech. 2025;70(11):417-426. doi: 10.17221/41/2025-VETMED.
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