Vet Med - Czech, 2026, 71(5):201-208 | DOI: 10.17221/73/2025-VETMED
Comparison of the effect of Mospilan SP and its active substance on Artemia franciscanaOriginal Paper
- 1 Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic
- 2 Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic
- 3 Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic
Contaminants formed after the use of pesticides raise concerns for human health and aquatic ecosystems, i. a., due to their high persistence in surface waters and their further migration to other components of the environment. In our study, we compared the effect of the insecticide Mospilan SP and its active substance acetamiprid on the aquatic crustacean Artemia franciscana. The concentration was 367.44 mg/l for Mospilan SP LC50 after 120 h, and 102.82 mg/l after 144 hours. The concentration was 148.04 mg/l for the active substance acetamiprid LC20 after 144 h and 28.84 mg/l after 168 hours. The commercial insecticide Mospilan SP showed a significant suppressive effect on the measured morphological parameters (body length and body width), with statistically significant differences observed after 10 days of exposure. When comparing the changes after the application of the insecticide and the active substance, it is impossible to confirm the same effect on the morphological parameters within the given time intervals. The objective of the study was to examine morphological changes at sublethal concentrations, as these changes can provide a more sensitive indication of the adverse effects of commercial products and active substances on morphological parameters.
Keywords: LC50; morphological changes; neonicotinoids
Received: September 24, 2025; Revised: January 24, 2026; Accepted: January 27, 2026; Published: May 27, 2026 Show citation
References
- Aliouane Y, El Hassani AK, Gary V, Armengaud C, Lambin M, Gauthier M. Subchronic exposure of honeybees to sublethal doses of pesticides: Effects on behavior. Environ Toxicol Chem. 2009 Jan;28(1):113-22.
Go to original source...
Go to PubMed... - Bartlett AJ, Hedges AM, Intini KD, Brown LR, Maisonneuve FJ, Robinson SA, Gillis PL, de Solla SR. Acute and chronic toxicity of neonicotinoid and butenolide insecticides to the freshwater amphipod, Hyalella azteca. Ecotoxicol Environ Saf. 2019 Jul 15;175:215-23.
Go to original source...
Go to PubMed... - Chen TH, Wang YH, Wu YH. Developmental exposures to ethanol or dimethylsulfoxide at low concentrations alter locomotor activity in larval zebrafish: Implications for behavioral toxicity bioassays. Aquat Toxicol. 2011 Apr;102(3-4):162-6.
Go to original source...
Go to PubMed... - Dvorak P, Benova K, Zdarsky M, Sklenar Z, Havelkova A. Use of the crustacean Artemia franciscana for alternative biotests. Acta Vet Brno. 2010;79(Suppl_9):S47-53.
Go to original source... - Dvorak P, Sucman E, Benova K. The development of a ten-day biotest using Artemia salina nauplii. Biologia (Bratisl). 2005;60(5):593-7.
- Finnegan MC, Baxter LR, Maul JD, Hanson ML, Hoekstra PF. Comprehensive characterization of the acute and chronic toxicity of the neonicotinoid insecticide thiamethoxam to a suite of aquatic primary producers, invertebrates, and fish. Environ Toxicol Chem. 2017 Oct;36(10):2838-48. Erratum in: Environ Toxicol Chem. 2018 Jan;37(1):285.
Go to original source...
Go to PubMed... - Goulson D; 232 signatories. Call to restrict neonicotinoids. Science. 2018 Jun 1;360(6392):973.
Go to original source...
Go to PubMed... - Hu G, Wang H, Shi H, Wan Y, Zhu J, Li X, Wang Q, Wang Y. Mixture toxicity of cadmium and acetamiprid to the early life stages of zebrafish (Danio rerio). Chem Biol Interact. 2022 Oct 1;366:110150.
Go to original source...
Go to PubMed... - Jeschke P, Nauen R. Neonicotinoid insecticides. In: Gilbert LI, Iatrou K, Gill SS, editors. Comprehensive molecular insect science. Vol. 3. Oxford: Elsevier; 2005. p. 53-105.
Go to original source... - Klingelhofer D, Braun M, Bruggmann D, Groneberg DA. Neonicotinoids: A critical assessment of the global research landscape of the most extensively used insecticide. Environ Res. 2022 Oct;213:113727.
Go to original source... - Luptakova L, Dvorcakova S, Demcisakova Z, Belbahri L, Holovska K, Petrovova E. Dimethyl sulfoxide: Morphological, histological, and molecular view on developing chicken liver. Toxics. 2021 Mar 12;9(3):55.
Go to original source... - Ma X, Li H, Xiong J, Mehler WT, You J. Developmental toxicity of a neonicotinoid insecticide, acetamiprid to zebrafish embryos. J Agric Food Chem. 2019 Mar 6;67(9):2429-36.
Go to original source...
Go to PubMed... - Malhotra N, Chen KH, Huang JC, Lai HT, Uapipatanakul B, Roldan MJM, Macabeo APG, Ger TR, Hsiao CD. Physiological effects of neonicotinoid insecticides on non-target aquatic animals - An updated review. Int J Mol Sci. 2021 Sep 4;22(17):9591.
Go to original source...
Go to PubMed... - Morrissey CA, Mineau P, Devries JH, Sanchez-Bayo F, Liess M, Cavallaro MC, Liber K. Neonicotinoid contamination of global surface waters and associated risk to aquatic invertebrates: A review. Environ Int. 2015 Jan;74:291-303.
Go to original source...
Go to PubMed... - OECD. Test No. 202: Daphnia sp. acute immobilisation test. OECD guidelines for the testing of chemicals, section 2. Paris: OECD Publishing; 2004. 12 p.
- Pietrzak D, Kania J, Kmiecik E, Malina G, Wator K. Fate of selected neonicotinoid insecticides in soil-water systems: Current state of the art and knowledge gaps. Chemosphere. 2020 Sep;255:126981.
Go to original source... - Prosser RS, de Solla SR, Holman EAM, Osborne R, Robinson SA, Bartlett AJ, Maisonneuve FJ, Gillis PL. Sensitivity of the early-life stages of freshwater mollusks to neonicotinoid and butenolide insecticides. Environ Pollut. 2016 Nov;218:428-35.
Go to original source... - Rico A, Arenas-Sanchez A, Pasqualini J, Garcia-Astillero A, Cherta L, Nozal L, Vighi M. Effects of imidacloprid and a neonicotinoid mixture on aquatic invertebrate communities under Mediterranean conditions. Aquat Toxicol. 2018 Nov;204:130-43.
Go to original source...
Go to PubMed... - SCHEER - Scientific Committee on Health, Environmental and Emerging Risks. Final opinion on draft environmental quality standards for priority substances under the Water Framework Directive - Acetamiprid [Internet]. Brussels: European Commission; 2022 Mar 25 [cited 2025 Aug 13]. Available from: https://health.ec.europa.eu/document/download/e4484454-5ce7-4135-9ce0-9f8e881c62cc_en?filename=scheer_o_024.pdf.
- Shi J, Yang H, Yu L, Liao C, Liu Y, Jin M, Yan W, Wu XB. Sublethal acetamiprid doses negatively affect the lifespans and foraging behaviors of honey bee (Apis mellifera L.) workers. Sci Total Environ. 2020 Oct 10;738:139924.
Go to original source...
Go to PubMed... - Shi X, Shi J, Yu L, Wu X. Metabolic profiling of Apis mellifera larvae treated with sublethal acetamiprid doses. Ecotoxicol Environ Saf. 2023 Apr 1;254:114716.
Go to original source...
Go to PubMed... - Simon-Delso N, Amaral-Rogers V, Belzunces LP, Bonmatin JM, Chagnon M, Downs C, Furlan L, Gibbons DW, Giorio C, Girolami V, Goulson D, Kreutzweiser DP, Krupke CH, Liess M, Long E, McField M, Mineau P, Mitchell EA, Morrissey CA, Noome DA, Pisa L, Settele J, Stark JD, Tapparo A, Van Dyck H, Van Praagh J, Van der Sluijs JP, Whitehorn PR, Wiemers M. Systemic insecticides (neonicotinoids and fipronil): Trends, uses, mode of action and metabolites. Environ Sci Pollut Res Int. 2015 Jan;22(1):5-34.
Go to original source... - Tomizawa M, Casida JE. Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors. Annu Rev Entomol. 2003;48:339-64.
Go to original source...
Go to PubMed... - Tomizawa M, Yamamoto I. Structure-activity relationships of nicotinoids and imidacloprid analogs. J Pestic Sci. 1993 Feb 20;18(1):91-8.
Go to original source... - UPI Cropscience. Material safety data sheet: Acetamiprid 20% SP [Internet]. 2022 [cited 2025 Aug 13]. Available from: https://lhyn-oss.oss-cn-beijing.aliyuncs.com/service/picture/1632642022060.pdf.
- Wexler P, Anderson B, de Peyster A, Gad SC, Hakkinen PJB, Kamrin M, Locey B, Mehendale HM, Pope C, Shugart LR, editors. Encyclopedia of toxicology. 2nd ed. Vol. 1. Oxford: Elsevier Academic Press; 2005. p. 88.
This is an open access article distributed under the terms of the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0.), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.

ORCID...