Vet Med - Czech, 2001, 46(2):29-34 | DOI: 10.17221/7848-VETMED

The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serumOriginal Paper

K. Kovařčík
Veterinary Research Institute, Brno, Czech Republic

We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation of the newly developed ELISA, field sera collected from 549 head of cattle in the Czech Republic were tested in parallel by a serum neutralization test. The tests showed 98.36% agreement. The specificity and sensitivity of the ELISA relative to serum neutralization test was 97.00% (226/233) and 99.37% (314/316), respectively. Tissue culture-grown viral antigen was used in the tests. The corrected optical density (COD) of each sample tested at dilution 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. We determined the relationship between the S/P ratio (%) obtained at a dilution 1/100 and the end point titer calculated by serum neutralization test (r = 0.9743). The ELISA test was evaluated by testing acute and convalescent (3 wk later) serum pairs from 9 head of cattle with confirmed BRSV infection for demonstration of seroconversion. The ELISA test demonstrated a clear increase of the S/P ratio (%) between acute and convalescent serum pairs (on average 42.2 ± 13.1).

Keywords: bovine respiratory syncytial virus (BRSV); respiratory tract pathogen; cattle; ELISA

Published: February 28, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kovařčík K. The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum. Vet Med - Czech. 2001;46(2):29-34. doi: 10.17221/7848-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Baker J.C., Werdin R.E., Ames T.R. (1986): Study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. J. Am. Vet. Med. Assoc., 189, 66–70. Go to original source... Go to PubMed...
    2. Cremer N.E., Cossen, C.K., Hanson C.V., Shell G.R. (1982): Evaluation and reporting of enzyme immunoassay determinations of antibody to herpes simplex virus in sera and cerebrospinal fluid. J. Clin. Microbiol., 15, 815–823. Go to original source... Go to PubMed...
    3. Edington N., Jacobs J.W. (1970): Respiratory syncytial virus in cattle. Vet. Rec., 87, 762.
    4. Ellis J.A., Hassard L.E., Morley P.S. (1995): Bovine respiratory syncytial virus-specific immune responses in calves after inoculation with commercially available vaccines. J. Am. Vet. Med. Assoc., 206, 354–361. Go to original source... Go to PubMed...
    5. Elvander M., Edwards S., Näslund K., Linde N. (1995): Evaluation and application of an indirect ELISA for the detection of antibodies to bovine respiratory syncytial virus in milk, bulk milk, and serum. J. Vet. Diagn. Invest., 7, 177–182. Go to original source... Go to PubMed...
    6. Florent G., Wiseman A. (1990): An IgM specific ELISA for the serodiagnostics of viral bovine infections. Comp. Immunol. Microbiol. Infect. Dis., 13, 203–208. Go to original source... Go to PubMed...
    7. Gillette K.G. (1983): Enzyme-linked immunosorbent assay used to monitor serum antibodies to bovine respiratory syncytial virus: comparison with complement fixation and neutralization tests. Am. J. Vet. Res., 44, 2251–2255.
    8. Henkel J.H., Aberle S.W., Kundi M., Popow-Kraupp T. (1997): Improved detection of respiratory syncytial virus in nasal aspirates by seminested RT-PCR. J. Med. Virol., 53, 366–371. Go to original source... Go to PubMed...
    9. Jacobs J.W., Edington N. (1971): Isolation of respiratory syncytial virus from cattle in Britain. Vet. Rec., 88, 694. Go to original source... Go to PubMed...
    10. Kimman T.G., Westebrink F., Schreuder B.E.C., Straver P.J. (1987): Local and systematic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies. J. Clin. Microbiol., 25, 1097–1106. Go to original source... Go to PubMed...
    11. Kovaøèík K. (1999): Isolation of bovine respiratory syncytial virus during an outbreak of acute respiratory disease in calves. Vet. Med. – Czech, 44, 121–127.
    12. Köves B., Bartha A. (1975): Isolation of respiratory syncytial (RS) virus from cattle diseased with respiratory symptoms. Acta Vet. Acad. Sci. Hung., 25, 357–362. Go to PubMed...
    13. McNulty M.S., Bryson D.G., Allan G.M. (1983): Experimental respiratory syncytial virus pneumonia in young calves: microbiologic and immunofluorescent findings. Am. J. Vet. Res., 44, 1656–1659.
    14. Paccaud M.F., Jaquier C. (1970): A respiratory syncytial virus of bovine origin. Arch. Ges. Virus Forsch., 30, 327–342. Go to original source... Go to PubMed...
    15. Pospíšil Z., Menšík J., Valíèek L. (1978): Isolation and identification of a bovine respiratory syncytial virus in Czechoslovakia. Acta Vet. Brno, 47, 79–86. Go to original source...
    16. Report of a Joint Food and Agriculture Organisation of the United Nations/International Atomic Energy Agency (FAO/ IAEA) Consultant’s Meeting on ELISA Data Evaluation and Expression, January 27–31, Vienna, 1992.
    17. Thomas L.H., Stott E.J. (1981): Diagnosis of respiratory syncytial virus infection in the bovine respiratory tract by immunofluorescence. Vet. Rec., 108, 432–435. Go to original source... Go to PubMed...
    18. Vilcek S., Elvander M., Ballagi-Pordány A., Belak S. (1994): Development of nested PCR assay detection of bovine respiratory syncytial virus in clinical specimens. J. Clin. Microbiol., 32, 2225–2231. Go to original source... Go to PubMed...
    19. Wellemans G., Leunen J., Luchsinger E. (1970): Isolement ïun virus (220/69) sérologiquement semblable au virus respiratoire (RS) humain. [Isolatin of a virus (220/69) serologically resembling human respiratory syncytial virus.] Ann. Méd. Vétér., 114, 89–93.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content