Vet Med - Czech, 2001, 46(3):65-69 | DOI: 10.17221/7854-VETMED

The effect of oral administration of salbutamol on the glycoconjugate composition in goblet cells of the tracheal epithelium in rabbitsOriginal Paper

L. Vajner, V. Konrádová, J. Uhlík, J. Zocová
1 Institute of Histology and Embryology,
2 nd Medical Faculty, 2Department of Applied Mathematics and Computer Science, Faculty of Science, Charles University, Prague, Czech Republic

We verified the influence of salbutamol on changes of glycoconjugates contained in tracheal goblet cells in rabbits by the oral administration of Ventolin™ syrup in the dose of 5 ml. Material for both conventional and lectin histochemistry was collected 15 and 30 minutes post exposure. Gradual decrease of percentage of goblet cells containing acid sialylated glycoconjugates was observed 15 minutes after administration. Thirty minutes after administration, neutral glycoconjugates-containing goblet cells were absent. The proportion of goblet cells containing acid sialylated glycoconjugates reached 50% of the value in control animals. Compared with controls, the changes of the character of the glycoconjugate content in the tracheal goblet cells due to the oral administration of Ventolin™ syrup were statistically significant ((α ≤ 0.01).

Keywords: tracheal epithelium; goblet cells; sialylated glycoconjugates; lectin histochemistry; rabbit; Ventolin syrup

Published: March 31, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Vajner L, Konrádová V, Uhlík J, Zocová J. The effect of oral administration of salbutamol on the glycoconjugate composition in goblet cells of the tracheal epithelium in rabbits. Vet Med - Czech. 2001;46(3):65-69. doi: 10.17221/7854-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Babál P., Pindak F.F., Wells D.J., Gardner W.A. (1994): Purification and characterization of sialic acid-specific lectin from Tritrichomonas mobilensis. Biochem. J., 299, 341–346. Go to original source... Go to PubMed...
    2. Babál P., Zaviaèiè M., Danihel 1/4., Gardner W.A., Jr. (1995): Histochemistry of the novel lectin from Tritrichomonas mobilensis with specificity for sialic acid. Bratisl. Lek. Listy, 96, 371–377. Go to PubMed...
    3. Babál P., Gardner W.A., Jr. (1996a): Histochemical localization of sialylated glycoconjugates with Tritrichomonas mobilensis lectin (TLM). Histol. Histopathol., 11, 621–631. Go to PubMed...
    4. Babál P., Slugeò I., Daniš D., Zaviaèiè M., Gardner W.A., Jr. (1996 b): Sialic acid expression in normal and diseased human kidney. Acta Histochem. (Jena), 98, 71–77. Go to original source... Go to PubMed...
    5. Castells M.T., Ballesta J., Pastor L.M., Madrid J.F., Marin J.A. (1990): Histochemical characterization of glycoconjugates in the epithelium of the extrapulmonary airways of several vertebrates. Histochem. J., 22, 24–35. Go to original source... Go to PubMed...
    6. Castells M.T., Ballesta J., Madrid J.F., Avilés M., MartínezMenárguez J.A. (1991): Characterization of glycoconjugates in developing rat respiratory system by means of conventional and lectin histochemistry. Histochemistry, 95, 419– 426. Go to original source... Go to PubMed...
    7. Castells M.T., Ballesta J., Madrid J.F., Martínez-Menárguez J.A., Avilés M. (1992): Ultrastructural localization of glycoconjugates in human bronchial glands: The subcellular organization of N- and O-linked oligosaccharide chains. J. Histochem. Cytochem., 40, 265–274. Go to original source... Go to PubMed...
    8. Castells M.T., Madrid J.F., Avilés M., Martínez-Menárguez J.A., Ballesta J. (1994): Cytochemical characterization of sulfo- and sialoglycoconjugates of human laryngeal glandular cells. J. Histochem. Cytochem., 42, 485–496. Go to original source... Go to PubMed...
    9. Damjanov I. (1987): Biology of disease. Lectin cytochemistry and histochemistry. Lab. Invest., 57, 5–20. Go to PubMed...
    10. Jacob S., Poddar S. (1982): Mucous cells of the tracheobronchial tree in the ferret. Histochemistry, 73, 599–605. Go to original source... Go to PubMed...
    11. Jeffery P.K., Gaillard D., Moret S. (1992): Human airway secretory cells during development and in mature airway epithelium. Eur. Respir. J., 5, 93–104. Go to original source...
    12. Jones R., Reid L. (1978): Secretory cells and their glycoproteins in health and disease. Brit. Med. Bull., 34, 9–16. Go to original source... Go to PubMed...
    13. Kiernan J.A. (1981): Histological and Histochemical Methods: Theory and Practice. Pergamon Press, Oxford. 344 pp.
    14. Konrádová V., Uhlík J., Vajner L., Zocová J. (1996): Vliv jednorázové aplikace Ventolinu na ultrastrukturu epitelu dýchacích cest. Ès. Pediat., 51, 725–728.
    15. Konrádová V., Uhlík J., Vajner L., Zocová J. (1998): The effect of two β2 adrenergic agonists on the ultrastructure of the airway epithelium in rabbits. Vet. Med. – Czech, 43, 187–192.
    16. Konrádová V., Vajner L., Uhlík J., Zocová J. (1999): Srovnání úèinku inhalace 2 vdechù Ventolinu a preparátu Placebo inhaler na epitel trachey u králíkù. Stud. Pneumol. Phthiseol., 59, 33–34.
    17. Konrádová V., Vajner L., Uhlík J., Zocová J. (2000a): Ultrastructure of the tracheal epithelium after oral administration of salbutamol. Vet. Med. – Czech, 45, 99–104.
    18. Konrádová V., Uhlík J., Vajner L., Zocová J. (2000b): Srovnání úèinku jedné terapeutické dávky Ventolinu aplikované per os nebo inhalaèní cestou na ultrastrukturu epitelu dýchacích cest. Èes.-slov. Pediatrie, 55, 271–275.
    19. Konrádová V., Vajner L., Uhlík J., Zocová J. (2000c): The effect of Placebo-Inhaler on the tracheal epithelium in rabbits. Vet. Med. – Czech, 45, 189–194.
    20. Lamb D., Reid L. (1972): Acidic glycoproteins produced by the mucous cells of the bronchial submucosal glands in the fetus and child: A histochemical study. Brit. J. Dis. Chest, 66, 248–253. Go to original source... Go to PubMed...
    21. Mandal C., Mandal C. (1990): Sialic acid binding lectins. Experientia, 46, 433–441. Go to original source... Go to PubMed...
    22. Mowry R.W., Winkler C.H. (1956): The coloration of acidic carbohydrates of bacteria and fungi in tissue sections with special reference to capsule of Cryptococcus neoformans, pneumococci, and staphylococci. Am. J. Pathol., 32, 628– 629.
    23. Sata T., Zuber C., Roth J. (1990): Lectin-digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry. Histochemistry, 94, 1–11. Go to original source... Go to PubMed...
    24. Shorten G.D., Dolovich M., Lerman J., Cutz E. (1995): Metered-dose inhaler salbutamol-induced tracheal epithelial lesions in intubated rabbits. Chest, 108, 1668–1672. Go to original source... Go to PubMed...
    25. Spahr-Schopfer I., Lerman J., Cutz E., Newhouse M.T., Dolovich, M. (1994): Proximate delivery of a large experimental dose from salbutamol MDI induces epithelial airway lesions in intubated rabbits. Am. J. Respir. Crit. Care Med., 150, 790–794. Go to original source... Go to PubMed...
    26. Spicer S.S., Schulte B.A., Thomopoulos G.N. (1983): Histochemical properties of the respiratory tract epithelium in different species. Am. Rev. Respir. Dis., 128, S20–S26. Go to PubMed...
    27. Vajner L. (1998): Úèinek dvou β2 sympatomimetických bronchospasmolytik na podíl sialovaných a sulfonovaných glykokonjugátù v sekretu pohárkových bunìk tracheálního epitelu. Ès. Patol., 34, 13–16. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content