Vet Med - Czech, 2001, 46(7):190-198 | DOI: 10.17221/7882-VETMED

Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influxOriginal Paper

Z. Sládek, D. Ryšánek, M. Faldyna
1 Mendel University of Agriculture and Forestry, Brno, Czech Republic
2 Veterinary Research Institute, Brno, Czech Republic

Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of leukocyte influx. Changes of percentage of leukocyte types during development and resolution of induced influx in comparison with blood leukocyte pattern allow identification of these cell types on FACS dot plot. The positions of mammary gland granulocyte and lymphocyte regions were identical with those of the corresponding peripheral blood cells. Two basic morphologically distinct types occupying separate regions in dot plots were observed in the population of mononuclear phagocytes (MoP): non-vacuolised monocyte-like macrophages (MoMAC) and vacuolised macrophages (MAC). Influx resolution was characterised by a marked shift of the MoMAC region towards that of MAC recognisable in dot plots by a separate region of intermediate MoP forms. The study provides a pattern of dynamics of percentages of mammary gland leukocyte types during influx development and resolution as imaged by FACS.

Keywords: flow cytometry; bovine virgin mammary gland; induced influx

Published: July 31, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sládek Z, Ryšánek D, Faldyna M. Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx. Vet Med - Czech. 2001;46(7-8):190-198. doi: 10.17221/7882-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bessis M. (1973): Living Blood Cells and Their Ultrastructure. Springer-Verlag, New York. 767 pp.
    2. Darzynkiewicz Z., Bruno S., Del Bino G., Gorczyca W., Hotz M.A., Lassota P., Traganos F. (1992): Features of apoptotic cells measured by flow cytometry. Cytometry, 13, 795-808. Go to original source... Go to PubMed...
    3. Derbyshire J.B., Berman D.T. (1968): Leukocytic responses of the bovine udder to infusion of certain irritants. Am. J. Vet. Res., 29, 1971-1977.
    4. Desiderio J.V., Campbell S.G. (1980): Bovine mammary gland macrophages: isolation, morphologic features, and cytophilic immunoglobulins. Am. J. Vet. Res., 41, 1595-1599.
    5. Emanuelson U., Wever P. (1989): Potential of differential somatic cell counts as indicators of mastitis in quarter milk samples from dairy cows. Acta Vet. Scand., 30, 475-481. Go to original source... Go to PubMed...
    6. Hageltorn M., Saad M.A. (1986): Flow cytofluorometric characterization of bovine blood and milk leukocytes. Am. J. Vet. Res., 47, 2012-2016.
    7. Jain N.C., Paape M.J., Miller R.H. (1991): Use of flow cytometry for determination of differential leukocyte counts in bovine blood. Am. J. Vet. Res., 52, 630-636. Go to original source...
    8. Matoušková O., Chalupa J., Cigler M., Hruška K. (1992): STAT Plus - Users Manual (in Czech). 1st ed. Veterinary Research Institute, Brno. 168 pp.
    9. Miller R.H., Paape M.J., Filep R., Link S. (1993): Flow cytometric analysis of neutrophils in cows' milk. Am. J. Vet. Res., 54, 1975-1979. Go to original source...
    10. Östensson K., Hageltorn M., Astrom G. (1988): Differential cell counting in fraction-collected milk from dairy cows. Acta Vet. Scand., 29, 493-500. Go to original source... Go to PubMed...
    11. Paape M.J., Pearson R.E., Wergin W.P., Guidry A.J. (1977): Enhancement of chemotactic response of polymorphonuclear leukocytes into the mammary gland and isolation from milk. J. Dairy Sci., 60, 53-62. Go to original source... Go to PubMed...
    12. Paape M.J., Lilius E.R.M., Wiitanen P.A., Kontio M.P., Miller R.H. (1996): Intramammary defense against infections induced by Escherichia coli in cows. Am. J. Vet. Res., 57, 477-482. Go to original source...
    13. Quiroga G.H., Sordillo L.M., Atkinson R.W., Nickerson S.C. (1993): Cytologic responses of Staphylococcus aureus-infected mammary glands of heifers to interferon gamma and interleukin-2 treatment. Am. J. Vet. Res., 54, 1894-1900. Go to original source...
    14. Redelman D., Butler S., Robison J., Garner D. (1988): Identification of inflammatory cells in bovine milk by flow cytometry. Cytometry, 9, 463-468. Go to original source... Go to PubMed...
    15. Ryšánek D., Šedivá A., Sládek Z., Babák V. (1999): Intramammary infections of juvenile mammary glands of heifers: absolute and differential somatic cell counts. Vet. Med. - Czech, 44, 199-203.
    16. Ryšánek D., Babák V., Sládek Z., Toman (2001): Variation among unbred heifers in the activities of their mammary gland and blood polymorphonuclear leukocytes. J. Vet. Med. B., 48, 31-42. Go to original source...
    17. Saad A.M., Östensson K. (1990): Flow cytometric studies on the alteration of leukocyte populations in blood and milk during endotoxin-induced mastitis in cows. Am. J. Vet. Res., 51, 1603-1607. Go to original source...
    18. Sanchez L., Aranda P., Perez M.D., Calvo M. (1988): Concentration of lactoferrin and transferrin throughout lactation in cow´s colostrum and milk. Biol. Chem. Hoppe Seyler, 369, 1005-1008. Go to original source... Go to PubMed...
    19. Sládek Z., Ryšánek D. (1999a): Morphological characteristic of somatic cells from mammary glands of unbred heifers. Vet. Med. - Czech, 44, 205-214.
    20. Sládek Z., Ryšánek D. (1999b): Ultrastructure of phagocytes from mammary glands of non-pregnant heifers. Anat. Histol. Embryol., 28, 291-298. Go to original source... Go to PubMed...
    21. Sládek Z., Ryšánek D. (2000a): Morphology of apoptosis of polymorphonuclear leukocytes isolated from mammary glands of unbred heifers. Vet. Med. - Czech, 45, 71-81.
    22. Sládek Z., Ryšánek D. (2000b): Apoptosis of polymorphonuclear leukocytes of the juvenile bovine mammary gland during induced influx. Vet. Res., 3, 553-563. Go to original source... Go to PubMed...
    23. Sládek Z., Ryšánek D. (2001): Neutrophil apoptosis during the resolution of bovine mammary gland injury. Res. Vet. Sci., 70, 41-46. Go to original source... Go to PubMed...
    24. Vermes I., Haanen C., Reutelingsperger C. (2000): Flow cytometry of apoptotic cell death. J. Immunol. Methods, 243, 167-190. Go to original source... Go to PubMed...
    25. Wardley R.C., Rouse B.T., Babiuk L.A. (1976): The mammary gland of the ox: a convenient source for the repeated collection of neutrophils and macrophages. J. Reticuloendothel. Soc., 19, 29-36. Go to PubMed...
    26. Wright S.D., Ramos R.A., Ulevitch R.J. (1989): Lipopolysaccharide (LPS) binding protein opsonizes LPS-bearing particles for recognition by a novel receptor on macrophages. J. Exp. Med., 70, 1231-1241. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content