Veterinární medicína, 2001 (vol. 46), issue 7-8

Apoptosis of neutrophilic granulocytes of bovine virgin mammary gland in scanning electron microscopyOriginal Paper

Z. Sládek, D. Ryšánek

Vet Med - Czech, 2001, 46(7):185-189 | DOI: 10.17221/7881-VETMED  

The objective of this work was the morphologic analysis of apoptosis of neutrophilic granulocytes (hereinafter referred to as neutrophils) in scanning electron microscopy (SEM) in comparison with morphological features distinguishable by light microscopy. This study was performed on 12 bovine virgin mammary glands washed with physiological buffered solution (PBS) prior to the induction of cell influx by PBS. Twenty-four hours after influx induction the cell suspension was obtained by the lavage of mammary glands with PBS. The particular lavages were cytologicaly and bacteriologicaly examined. all bacteriological examinations were negative. Mononuclear...

Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influxOriginal Paper

Z. Sládek, D. Ryšánek, M. Faldyna

Vet Med - Czech, 2001, 46(7):190-198 | DOI: 10.17221/7882-VETMED  

Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of leukocyte influx. Changes of percentage of leukocyte types during development and resolution of induced influx in comparison with blood leukocyte pattern allow identification of these cell types on FACS dot plot. The positions of mammary gland granulocyte and lymphocyte regions were identical with those of the corresponding peripheral blood cells. Two basic morphologically distinct types occupying separate regions in dot plots were observed in the population...

Detection of neoplastic cells in blood of miniature pigs with hereditary melanomaOriginal Paper

P. Pohlreich, J. Stříbrná, Z. Kleibl, V. Horák, J. Klaudy

Vet Med - Czech, 2001, 46(7):199-204 | DOI: 10.17221/7880-VETMED  

Tyrosine, a key enzyme of melanin biosynthesis, is widely used as a specific marker for the detection of dissemination or metastatic melanoma cells in peripheral blood and other tissues like lymph node or bone marrow, which are normally tyrosinase negative. The amplification of tyrosinase-specific mRNA by means of RT-PCR is a sensitive technique capable of detecting a single tumour cell in 5-10 ml of whole blood. We have utilised this method to analyse the peripheral blood of laboratory miniature pigs with advanced cutaneous melanoma for the presence of tumor cells. This highly invasive hereditary malignancy can serve as an experimental model for the...

The transmission and impact of paratuberculosis infection in domestic and wild ruminantsReview

W.y Ayele, M. Macháčková, I. Pavlík

Vet Med - Czech, 2001, 46(7):205-224 | DOI: 10.17221/7878-VETMED  

Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) infects domestic cattle, sheep, goats, deer, camelids and wild ruminants leading to chronic enteritis known as paratuberculosis (Johne's disease). The infection is chronic, progressive and unresponsive to treatment. Most infected animals do not develop clinical disease but may excrete the bacteria. Clinically sick animals suffer emaciation and in some species diarrhoea, followed by eventual death. During the course of the disease, excretion of M. paratuberculosis in faeces and milk occurs, and the organism spreads through the blood and lymph vessels...

Emergency control of transboundary diseases of livestock in Southern and Eastern EuropeReview

K.J. Wojciechowski, R. Paskin, L. Pite, K. Hruška

Vet Med - Czech, 2001, 46(7):225-228 | DOI: 10.17221/7879-VETMED  

"Emergency Control of Transboundary Diseases of Livestock in Southern and Eastern Europe", approved in the year 2000 and implemented from September 2001 the CENTAUR is expected to expand significantly. The major objective of this training Regional Project will be to strengthen the national and regional capacities to respond to transboundary animal disease emergencies through technology transfer in the fields of diagnostics, epidemiology and emergency preparedness. The following countries are included in the Project: Albania, Bosnia-Herzegovina (the Federation and the Serbian Republic), Bulgaria, Croatia, FYR Macedonia, Moldova, Romania, Slovenia and...