Vet Med - Czech, 2004, 49(2):35-41 | DOI: 10.17221/5672-VETMED

Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae

I. Holko, J. Urbanova, Tholkova, V. Kmet
2 , T. H 1, V. K 3
1 State Veterinary Institute, Prague, Czech Republic
2 State Veterinary Institute, Dolny Kubin, Slovak Republic
3 Institute of Animal Physiology of the Slovak Academy of Sciences, Kosice, Slovak Republic

The main goal of our work is the presentation and analysis of incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of all cases (98) with clinical signs of respiratory infections that we examined in the course of one year. We present also one of possibilities to the solution of problematic detection of M. hyopneumoniae which is, like the whole rank of mycoplasmas, very difficult to cultivate. This problem was solved by using the PCR method with the direct isolation of M. hyopneumoniae from lungs tissue. In antibiotic sensitivity testing of Pasteurella multocida and Actinobacillus pleuropneumoniae resulted enrofloxacin as the most effective antibiotics in the therapy of PRDC regarding bacterial agents.in above mentioned territory.

Keywords: pigs; Pasteurella multocida; Actinobacillus pleuropneumoniae; Mycoplasma hyopneumoniae; antibiotic sensitivity; PCR

Published: February 29, 2004  Show citation

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Holko I, Urbanova J, Tholkova, Kmet V. Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae. Vet Med - Czech. 2004;49(2):35-41. doi: 10.17221/5672-VETMED.
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    References

    1. Armstrong C.H., Scheidt A.B., Thacker E.L., Runnels L.J., Freeman M.J. (1984): Evaluation of criteria of the postmortem diagnosis of mycoplasmal pneumonia of swine. Mol. Cell. Probes, 7, 381-385.
    2. Baumeister K., Runge M., Ganter M., Feenstra A., Delbeck F., Kirchhoff H. (1998): Detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by PCR. J. Clin. Microbiol., 36, 1984-1988. Go to original source... Go to PubMed...
    3. Bolske G., Strandberg L., Bergstrom K., Johansson K. (1987): Species-specific antigen of Mycoplasma hyopneumoniae and cross-reaction with other porcine mycoplasmas. Curr. Microbiol., 15, 233-239. Go to original source...
    4. Friis N.F. (1975): Some recommendations concerning primary isolation of Mycoplasma hyopneumoniae and Mycoplasma flocculare. Nord. Veterinaermed., 27, 337-339.
    5. Gilbride K.A., Rosendal S. (1983): Evaluation of a selective medium for isolation of Haemophilus pleuropneumoniae. Can. J. Comp. Med., 47, 445-450.
    6. Harasawa R., Koshimiyu K., Takeda O., Uemori T., Asada K., Kato I. (1991): Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction. Mol. Cell. Probes, 5, 103-109. Go to original source... Go to PubMed...
    7. Hoie S., Falk K., Limm B.M. (1991): An abbatoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. IV. Bacteriological findings in chronic pneumonic lesions. Acta. Vet. Scand., 32, 395-402. Go to original source... Go to PubMed...
    8. Holt J., Krieg N., Sneath P., Staley J., Williams S. (1994): Bergey's Manual of Determinative Bacteriology. 9th ed. Williams &Wilkins, U.S.A. 566 pp.
    9. Innoue A., Yamamoto K., Hirano N., Murakami T. (1984): Drug susceptibility of Haemophilus pleuropneumoniae strains isolated from pigs. Jpn. Vet. Sci., 46, 175-180. Go to original source... Go to PubMed...
    10. Kamp E., Bokken G., Vermeulen T.F., de Jong M., Buys H., Reek F., Smits M. (1996): A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs. J. Vet. Diagn. Invest., 8, 304-309. Go to original source... Go to PubMed...
    11. Kauffman P., Wu W., Kim D., Cseke L. (1995): Handbook of Molecular and Cellular Methods in Biology and Medicine. CRC Press, U.S.A. 2-4 and 266.
    12. Lichtensteiger C., Steenbergen S., Lee R., Polson D., Vimr E. (1996): Direct PCR analysis for toxigenic Pasteurella multocida. J. Clin. Microbiol., 34, 3035-3039. Go to original source... Go to PubMed...
    13. Ma sson J.G., Bergstrom K., Wallgren P., Johansson K.E. (1995): Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene. J. Clin. Microbiol., 33, 893-897. Go to original source... Go to PubMed...
    14. Nadeau M., Lariviere S., Higgins R., Martineau G.P. (1988): Minimal inhibitory concentrations of antimicrobial agents against Actinobacillus pleuropneumoniae. Can. J. Vet. Res., 52, 315-318.
    15. NCCLS - The National Commi ee for Clinical Laboratory Standards (1997): Performance standards for antimicrobial disc and dilution susceptibility tests for bacteria isolated from animals; Tentative Standard, 17, 1-64.
    16. Nicolet J. (1988): Taxonomy of serological identification of Actinobacillus pleuropneumoniae. Can. Vet. J., 29, 578-579. Go to PubMed...
    17. Nicolet J. (1992): Actinobacillus pleuropneumoniae. In: Leman A.D., Straw B.E., Mengeling W.L., D'Allaire S., Taylor D.J. (eds.): Diseases of Swin. 7th ed. Iowa State University Press, Ames. 401-408.
    18. Nicolet J., Schifferli D. (1982): In vitro susceptibility of Haemophilus pleuropneumoniae in antimicrobial substances. Proc. Int. Congr. Pig Vet. Soc., 7, 71.
    19. Pijoan C., Fuentes M. (1987): Severe pleuritis associated with certain strains of Pasteurella multocida in swine. J. Am. Vet. Med. Assoc., 191, 823-826. Go to original source... Go to PubMed...
    20. Pijoan C., Lastra A., Ramirez C., Leman A. (1984): Isolation of toxigenic strains of Pasteurella multocida from lungs of pneumonic swine. J. Am. Vet. Med. Assoc., 185, 522-523. Go to original source... Go to PubMed...
    21. Presco J.F., Baggot J.D. (1993): Antimicrobial Therapy in Veterinary Medicine. Iowa State University Press, Ames.
    22. Ross R.F. (1999): Mycoplasmal diseases. In: Leman A.D., Straw B.E., Mengeling W.L., Allaire S.D., Taylor D.J. (eds.): Diseases of Swine. Iowa State University Press, Ames. 495-511.
    23. Rubies X., Casal J., Fernandez J., Pijoan C. (1996): Transmission of Pasteurella multocida clones in a swine pyramid structure. Proc. Int. Congr. Pig Vet. Soc., 14, 243.
    24. Satran P., Bartos M., Nedbalcova K., Rychlik I., Konstantinova L., Ulmann L. (1999): Identification of toxigenic strains of Pasteurella multocida by PCR and biological methods. Vet. Med. - Czech, 44, 275-278.
    25. Satran P., Nedbalcova K. (2002): Prevalence of serotypes, production of Apx toxins, and antibiotic resistance in strains of Actinobacillus pleuropneumoniae isolated in the Czech Republic. Vet. Med. - Czech, 47, 92-98. Go to original source...
    26. Sebunya T.N., Saunders J.R. (1983): Haemophilus pleuropneumoniae infection in swine. A review J. Am. Vet. Med. Assoc., 182, 1331-1336. Go to original source... Go to PubMed...

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