Vet Med - Czech, 2009, 54(1):12-18 | DOI: 10.17221/3081-VETMED

Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

R. Smitalova, L. Rodak, B. Smid, I. Psikal
Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic

Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain.

Keywords: group B rotavirus; group C rotavirus; nongroup A rotavirus; enteritis; RT-PCR, semi-nested PCR

Published: January 31, 2009  Show citation

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Smitalova R, Rodak L, Smid B, Psikal I. Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic. Vet Med - Czech. 2009;54(1):12-18. doi: 10.17221/3081-VETMED.
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