Veterinární medicína, 2009 (vol. 54), issue 1

Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents

C. Herrera-Luna, D. Klein, G. Lapan, S. Revilla-Fernandez, B. Haschek, I. Sommerfeld-Stur, K. Moestl, W. Baumgartner

Vet Med - Czech, 2009, 54(1):1-11 | DOI: 10.17221/3080-VETMED  

Faecal samples from 230 diarrhoeic and healthy calves aged 0-6 weeks, from 100 farms in Austria, were examined between October 2004 and February 2005 for the presence of bacteria, especially Shiga toxin-producing Escherichia coli (STEC), viruses and parasites. Escherichia coli was detected in 17% of all the faecal samples and was more prevalent in healthy calves. However, E. coli F5 was identified only in one calf without diarrhoea. Overall, 35 out of the 230 (15.2%) samples analyzed carried the Shiga toxin gene: stx1, stx2 or both stx1 and stx2 in their faeces, STEC. Nevertheless, out of 39 pathogenic E....

Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

R. Smitalova, L. Rodak, B. Smid, I. Psikal

Vet Med - Czech, 2009, 54(1):12-18 | DOI: 10.17221/3081-VETMED  

Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison...

Effect of triacylglycerols of medium-chain fatty acids on growth rate and mortality of rabbits weaned at 25 and 35 days of age

E. Skrivanova, V. Skrivanova, Z. Volek, M. Marounek

Vet Med - Czech, 2009, 54(1):19-24 | DOI: 10.17221/3079-VETMED  

Three hundred Hyplus rabbits reared on a commercial farm were weaned at the age of 25 days and 300 rabbits were weaned at the age of 35 days. Early-weaned and conventionally weaned rabbits were assigned to three groups and fed diets supplemented with protected palm fat (PPF), coconut oil (CO) and an oil containing triacylglycerols (TAG) of caprylic and capric acid (Akomed R) at 10 g/kg. The principal fatty acids in these supplements were palmitic, lauric and caprylic acid, respectively. Diets were fed until slaughter at 77 days of age. Mortality of early-weaned rabbits fed the diet supplemented with oil containing TAG of caprylic and capric acid (23%)...

Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius)

M. El-Boshy, H. Abbas, S. El-Khodery, S. Osman

Vet Med - Czech, 2009, 54(1):25-32 | DOI: 10.17221/3044-VETMED  

The present study had the aim of assessing the cytokine response and selected clinicopathological findings associated with brucellosis in camels (Camelus dromedarius). 340 dromedary camels were examined for brucellosis using agglutination and Complement Fixation tests (CFT). Twenty-five camels (7.35%) were positive by both tests; 14 (4.12%) for B. abortus and 11 (3.23%) for B. melitensis. IL-1β and IL-10 interleukin levels in both B. abortus and B. melitensis infected camels showed significant elevations (P < 0.05) compared with controls. Moreover, there was significantly larger increase in IL-1β...

Evaluation of the activity of intraarticular hyaluronic acid in the repair of experimentally induced osteochondral defects of the stifle joint in dogs

A. Sagliyan, E. Karabulut, E. Unsaldi, I. Yaman

Vet Med - Czech, 2009, 54(1):33-40 | DOI: 10.17221/3043-VETMED  

The present study examined the results of using hyaluronic acid with autogenetic cancellous grafts in the treatment of experimentally induced osteochondral defects in the stifle joints of dogs. In this study, 10 mature dogs of different breeds, weights and of both sexes were used. General anesthesia and usual operation procedures were followed. A 10 mm deep defect was created on the femoral sulcus of the trochlea with a drill tip of 8 mm in diameter. The defects in the right and left legs were filled with autogenic cancellous grafts taken from the metaphysial region of the tibia. The left legs constituted the experimental group while the right legs...