Vet Med - Czech, 2009, 54(1):25-32 | DOI: 10.17221/3044-VETMED

Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius)

M. El-Boshy1, H. Abbas2, S. El-Khodery1, S. Osman3
1 Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
2 Animal Health Research Institute, Cairo, Egypt
3 Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt

The present study had the aim of assessing the cytokine response and selected clinicopathological findings associated with brucellosis in camels (Camelus dromedarius). 340 dromedary camels were examined for brucellosis using agglutination and Complement Fixation tests (CFT). Twenty-five camels (7.35%) were positive by both tests; 14 (4.12%) for B. abortus and 11 (3.23%) for B. melitensis. IL-1β and IL-10 interleukin levels in both B. abortus and B. melitensis infected camels showed significant elevations (P < 0.05) compared with controls. Moreover, there was significantly larger increase in IL-1β interleukins in camels infected with B. abortus compared with B. melitensis. TNF-α, IFN-γ and IL-1α levels showed significant decreases (P < 0.05) in Brucella infected camels compared with non-infected ones; however, there was non-significant changes in IL-6 levels in Brucella infected camels compared with controls. Lymphopenia was recorded in infected camels but not in controls. However, normocytic normochromic anemia, hypoproteinemia, hypoalbuminemia and hypoglycemia were recorded in the B. abortus group only. Sorbitol dehydrogenase (SD), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed significant increases (P < 0.05) in infected camels compared with controls, and in B. abortus infected camels compared with B. melitensis infected animals. This is the first report that describes changes in selected cytokines and various haematological and biochemical parameters associated with brucellosios in dromedary camels. Emphasis should be placed on multidisciplinary research to elucidate the immunomodulatory features of camel brucellosis.

Keywords: camel; Brucellosis; cytokines; hematology; biochemical parameters; Egypt

Published: January 31, 2009  Show citation

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El-Boshy M, Abbas H, El-Khodery S, Osman S. Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Vet Med - Czech. 2009;54(1):25-32. doi: 10.17221/3044-VETMED.
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    References

    1. Abu Damir H., Tageldin M.H., Kenyon S.J. Idris O.F. (1989): Isolation of Brucella abortus from experimentally infected dromedary camels in Sudan: a preliminary report. Veterinary Research Communications, 13, 403-406. Go to original source... Go to PubMed...
    2. Adams L.G. (2002): The pathology of brucellosis reflects the outcome of the battle between the host genome and the Brucella genome. Veterinary Microbiology, 90, 553-561. Go to original source... Go to PubMed...
    3. Afzal M., Sakkir M. (1994): Survey of antibodies against various infectious disease agents in racing camels in Abu Dhabi, United Arab Emirates. Review Science and Technology, 13, 787-792. Go to original source... Go to PubMed...
    4. Akbulut, H., Celik, I., Akbulut A. (2007): Cytokine levels in patients with brucellosis and their relations with the treatment. Indian Journal of Medical Microbiology, 25, 387-390. Go to original source... Go to PubMed...
    5. Al-Majali A.M., Al-Qudah K.M., Al-Tarazi Y.H., AlRawashdeh O.F. (2008): Risk factors associated with camel brucellosis in Jordan. Tropical Animal Health and Production, 40, 193-200. Go to original source... Go to PubMed...
    6. Al-Shamahy H.A. (1999): Seropositivity for brucellosis in a sample of animals in the Republic of Yemen. Eastern Mediterranean Health Journal, 5, 1035-1041. Go to original source... Go to PubMed...
    7. Alton G.G., Jones L.M., Angus R.D.,Verger J.M. (1988): Techniques for the Brucellosis Laboratory. INRA, Paris. 123-130.
    8. Baumann M.P., Zessin K.H. (1992): Productivity and health of camels (Camelus dromedarius) in Somalia: associations with trypanosomosis and brucellosis. Tropical Animal Health and Production, 24, 145-156. Go to original source... Go to PubMed...
    9. Braun U., Pospischil A., Pusterla N., Winder C. (1995): Ultrasonographic findings in cows with cholestasis. Veterinary Record, 18, 537-543. Go to original source... Go to PubMed...
    10. Budak F., Goral G., Heper Y., Yilmaz E., Aymak F., Basturk B., Tore O., Ener B., Oral H.B. (2007): IL-10 and IL-6 gene polymorphisms as potential host susceptibility factors in Brucellosis. Cytokine, 38, 32-36. Go to original source... Go to PubMed...
    11. Cooper C.W. (1991): The epidemiology of human brucellosis in a well defined urban population in Saudi Arabia. Journal of Tropical Medicine and Hygiene, 94, 416-422. Go to PubMed...
    12. Damir H.A., Kenyon S.J., Khalaf Alla A.E., Idris O.F. (1984): Brucella antibodies in Sudanese camels. Tropical Animal Health and Production, 16, 209-212. Go to original source... Go to PubMed...
    13. Dash P.K., Joshi H.C. (1989): Clinico-biochemical studies on acute toxic nephropathy in goats due to uranyl nitrate. Veterinary and Human Toxicology, 31, 5-9.
    14. Demirdag K., Ozden M., Kalkan A., Godekmerdan A., Sirri Kilic S. (2003): Serum cytokine levels in patients with acute brucellosis and their relation to the traditional inflammatory markers. FEMS Immunology Medical Microbiology, 28,149-153. Go to original source... Go to PubMed...
    15. Dinarello C.A. (1996): Biologic basis for interleukin-1 in disease. Blood, 87, 2095-2147. Go to original source...
    16. Dinarello C.A. (2005): Blocking IL-1 in systemic inflammation. Journal of Experimental Medicine, 2, 1355-1359. Go to original source... Go to PubMed...
    17. Dornand J., Gross A., Lafont V., Liautard J., Oliaro J., Liautard J.P. (2002): The innate immune response against Brucella in humans. Veterinary Microbiology, 20, 383-394. Go to original source... Go to PubMed...
    18. Dzata G.K., Confer A.W., Wyckoff J.H. (1991): The effects of adjuvant on immune responses in cattle injected with a Brucella abortus soluble antigen. Veterinary Microbiology, 29, 27-48. Go to original source... Go to PubMed...
    19. Enright F.M., Walker J.V., Jeffers G., Deyoe B.L. (1984): Cellular and humoral responses of Brucella abortusinfected bovine fetuses. American Journal of Veterinary Research, 45, 424-430.
    20. Erdogan S., Celik S., Aslantas O., Kontas T., Ocak S. (2007): Elevated cAMP levels reverse Brucella melitensis-induced lipid peroxidation and stimulate IL-10 transcription in rats. Research in Veterinary Science, 82, 181-186. Go to original source... Go to PubMed...
    21. Fernandes D.M., Baldwin C.L. (1995): Interleukin-10 downregulates protective immunity to Brucella abortus. Infection and Immunity, 63, 1130-1133. Go to original source... Go to PubMed...
    22. Fernandez-Lago L., Orduna A., Vizcaino N. (2005): Reduced interleukin-18 secretion in Brucella abortus 2308-infected murine peritoneal macrophages and in spleen cells obtained from B. abortus 2308-infected mice. Journal of Medical Microbiology, 54, 527-531. Go to original source... Go to PubMed...
    23. Gameel S.E.A.M., Mohammed S.O., Mustafa A.A., Azwai S.M. (1993): Prevalence of camel brucellosis in Libya. Tropical Animal Health and Production, 25, 91-93. Go to original source... Go to PubMed...
    24. Giambartolomei G.H., Delpino M.V., Cahanovich M.E., Wallach J.C., Baldi P.C., Velikovsky C.A., Fossati C.A. (2002): Diminished production of T helper 1 cytokines correlates with T cell unresponsiveness to Brucella cytoplasmic proteins in chronic human brucellosis. Journal of Infectious Diseases, 15, 252-259. Go to original source... Go to PubMed...
    25. Hamdy M.F., Amin A.S. (2002): Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Veterinary Journal, 163, 299-305. Go to original source... Go to PubMed...
    26. Hegazy A.A., El Dughaym A., Alaknah M., Housawi F.M.T., Hatem M.E. (2004): Studies on mastitis in female camel with special reference to brucellosis. Journal of Camel Science, 1, 96-102.
    27. Hoover D.L., Crawford R.M., Van De Verg L.L., Izadjoo M.J., Bhattacharjee A.K., Paranavitana C.M., Warren R.L., Nikolich M.P., Hadfield T.L. (1999): Protection of mice against brucellosis by vaccination with Brucella melitensis WR201 (16MDeltapurEK). Infection and Immunity, 67, 5877-5884. Go to original source... Go to PubMed...
    28. Kalaitzakis E., Roubies N., Panousis N., Pourliotis K., Kaldrymidou E., Karatzias H. (2007): Clinicopathologic evaluation of hepatic lipidosis in periparturient dairy cattle. Journal of Veterinary Internal Medicine, 21, 835-845. Go to original source... Go to PubMed...
    29. Kudi A.C., Kalla D.J.U., Kudi M.C., Kapio G.I. (1997): Brucellosis in camels. Journal of Arid Environments, 37, 413-417. Go to original source...
    30. Lee Y., Park B.C., Lee Do H., Bae K.H., Cho S., Le C.H., Lee J.S., Myung P.K., Park S.G. (2006): Mouse transthyretin-related protein is a hydrolase which degrades 5-hydroxyisourate, the end product of the uricase reaction. Molecular Cells, 31, 141-145. Go to original source...
    31. Mantur B.G., Amarnath S., Shinde R.S. (2007): Review of clinical and laboratory features of human Brucellosis. Indian Journal of Medical Microbiology, 25, 188-202. Go to original source...
    32. Moore K.W., de Waal Malefyt R., Coffman R.L., O'Garra A. (2001): Interleukin-10 and the interleukin-10 receptor. Annual Review of Immunology, 19, 683-765. Go to original source... Go to PubMed...
    33. Murphy E.A., Sathiyaseelan J., Parent M.A., Zou B., Baldwin C.L. (2001): Interferon-gamma is crucial for surviving a Brucella abortus infection in both resistant C57BL/6 and susceptible BALB/c mice. Immunology, 103, 511-518. Go to original source... Go to PubMed...
    34. Musa M.T., Eisa M.Z., El Sanousi E.M., Abdel Wahab M.B., Perrett L. (2008): Brucellosis in Camels (Camelus dromedarius) in Darfur, Western Sudan. Journal of Comparative Pathology, 138, 151-155. Go to original source... Go to PubMed...
    35. Nicklin M.J., Hughes D.E., Barton J.L., Ure J.M., Duff G.W. (2000): Arterial inflammation in mice lacking the interleukin 1 receptor antagonist gene. Journal of Experimental Medicine, 191, 303-312. Go to original source... Go to PubMed...
    36. Odbileg R., Konnai S., Ohashi K., Onuma M. (2005): Molecular cloning and phylogenetic analysis of inflammatory cytokines of Camelidae (llama and camel). Journal of Veterinary Medical Science, 67, 921-925. Go to original source... Go to PubMed...
    37. Odbileg R., Purevtseren B., Batsukh Z., Konnai S., Ohashi K., Onuma M. (2006): Complete cDNA sequences and phylogenetic analyses of the Th1 and Th2 cytokines of the bactrian camel (Camelus bactrianus). Journal of Veterinary Medical Science, 68, 941-946. Go to original source... Go to PubMed...
    38. Odbileg R., Purevtseren B., Gantsetseg D., Boldbaatar B., Buyannemekh T., Galmandakh Z., Erdenebaatar J., Konnai S., Onuma M., Ohashi K. (2008): Cytokine responses in camels (Camelus bactrianus) vaccinated with Brucella abortus strain 19 vaccine. Journal of Veterinary Medical Science, 70, 197-201. Go to original source... Go to PubMed...
    39. Omer M.K., Skjerve E., Holstad G., Woldehiwet Z., Macmillan A.P. (2000): Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems. Epidemiology and Infection, 125, 447-453. Go to original source... Go to PubMed...
    40. Palmer M.V., Cheville N.F., Tatum F.M. (1996): Morphometric and histopathologic analysis of lymphoid depletion in murine spleens following infection with Brucella abortus strains 2308 or RB51 or an htrA deletion mutant. Veterinary Pathology, 33, 282-289. Go to original source... Go to PubMed...
    41. Pasquali P., Adone R., Gasbarre L.C., Pistoia C., Ciuchini F. (2001): Mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B. abortus 2308 infection. Infection and Immunity, 69, 6541-6544. Go to original source... Go to PubMed...
    42. Radostits O.M., Gay C.C., Hinchcliff K.W., Constable P.D. (2007): Veterinary Medicine. 10th ed. Elsevier Saunders, London. 389-390.
    43. Radwan A.I., Bekairi S.I., Mukayel A.A., Al-Bokmy A.M., Prasad P.V., Azar F.N., Coloyan E.R. (1995): Control of Brucella melitensis infection in a large camel herd in Saudi Arabia using antibiotherapy and vaccination with Rev. 1 vaccine. Review of Science and Technology, 14, 719-732. Go to original source... Go to PubMed...
    44. Sarafidis K., Diamanti E., Agakidis Ch., Karantaglis N., Roilidies E. (2006): Congenital brucellosis: a case report and review. In: 1st International Meeting on the Treatment of Human Brucellosis, Ioannina, Greece, 7-10.
    45. Saunders B.M., Liu Z., Zhan Y., Cheers C. (1993): Interleukin-6 production during chronic experimental infection. Immunolgy and Cell Biology, 71, 275-280. Go to original source... Go to PubMed...
    46. Saunders B.M., Frank A.A., Orme I.M., Cooper A.M. (2000): Interleukin-6 induces early gamma interferon production in the infected lung but is not required for generation of specific immunity to Mycobacterium tuberculosis infection. Infection and Immunity, 68, 3322-3326. Go to original source... Go to PubMed...
    47. Schelling E., Diguimbaye C., Daoud S., Nicolet J., Zinsstag J. (2004): Seroprevalences of zoonotic diseases in nomads and their livestock in Chari-Baguirmi, Chad. Medicine Tropics, 64, 474-477.
    48. Seder R.A., Hill A.V. (2000): Vaccines against intracellular infections requiring cellular immunity. Nature, 17, 793-798. Go to original source... Go to PubMed...
    49. Stevens M.G., Olsen S.C. (1994): In vitro effects of live and killed Brucella abortus on bovine cytokine and prostaglandin E2 production. Veterinary Immunology and Immunopathollogy, 40, 149-161. Go to original source... Go to PubMed...
    50. Stevens M.G., Olsen S.C., Pugh G.W.J., Palmer M.V. (1994): Immune and pathologic responses in mice infected with Brucella abortus 19, RB51, or 2308. Infection and Immunity, 62, 3206-3212. Go to original source... Go to PubMed...
    51. Teshome H., Molla B., Tibbo M. (2003): A seroprevalence study of camel brucellosis in three camel-rearing regions of Ethiopia. Tropical Animal Health and Production, 35, 381-390. Go to original source... Go to PubMed...
    52. Trinchieri G. (2007): Interleukin-10 production by effector T cells: Th1 cells show self control. Journal of Experimental Medicine, 19, 239-243. Go to original source... Go to PubMed...
    53. World Health Organization (WHO) (1986): 6th Report of the Joint FAO/WHO Expert Committee on Brucellosis. WHO Technical Reports Series, 740. WHO, Geneva.
    54. Wyckoff J.H. 3rd., Howland J.L., Scott C.M., Smith R.A., Confer A.W. (2005): Recombinant bovine interleukin 2 enhances immunity and protection induced by Brucella abortus vaccines in cattle. Veterinary Microbiology, 30, 77-87. Go to original source... Go to PubMed...
    55. Yagoub I.A., Mohamed A.A., Salim M.O. (1990): Serological survey of Brucella abortus antibody prevalence in the one-humped camel (Camelus dromedarius) from eastern Sudan. Revue D'elevage et de Medecine Veterinaire des Pays Tropicaux, 43, 167-171. Go to original source... Go to PubMed...
    56. Zhan Y., Cheers C. (1993): Endogenous gamma interferon mediates resistance to Brucella abortus infection. Infection and Immunity, 61, 4899-4901. Go to original source... Go to PubMed...
    57. Zhan Y., Liu Z., Cheers C. (1996): Tumor necrosis factor alpha and interleukin-12 contribute to resistance to the intracellular bacterium Brucella abortus by different mechanisms. Infection and Immunity, 64, 2782-2786. Go to original source... Go to PubMed...

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