Vet Med - Czech, 2010, 55(2):87-90 | DOI: 10.17221/25/2010-VETMED

Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report

P. Kriz, I. Slana, V. Mrlik, M. Moravkova, A. Kralova, K. Krizova, I. Pavlik
Veterinary Research Institute, Brno, Czech Republic

We report three pigeons euthanized in a small household breeding facility, where there was suspicion of an avian tuberculosis outbreak. For rapid identification of Mycobacterium avium subsp. avium direct conventional multiplex PCR was used. Nodular lesions were found on the livers of all three birds, the intestine of one bird and the kidney and ovaries of another. The liver samples and a further 18 tissue samples were examined. Acid-fast rods were detected in all the tissue samples after Ziehl-Neelsen staining. Isolation and diagnosis of M. a. avium (serotype 1 containing IS901) from 17 tissue samples was confirmed using conventional multiplex PCR.

Keywords: avian tuberculosis; mycobacteriosis; zoonosis; outbreak; rapid detection

Published: February 28, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kriz P, Slana I, Mrlik V, Moravkova M, Kralova A, Krizova K, Pavlik I. Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report. Vet Med - Czech. 2010;55(2):87-90. doi: 10.17221/25/2010-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anonymous (1999): Act No. 166/1999 Coll., on veterinary care and on a change of some related laws (Veterinary Act).
    2. Bartos M, Hlozek P, Svastova P, Dvorska L, Bull T, Matlova L, Parmova I, Kuhn I, Stubbs J, Moravkova M, Kintr J, Beran V, Melicharek I, Ocepek M, Pavlik I (2006): Identification of members of Mycobacterium avium species by Accu-Probes, serotyping, and single IS900, IS901, IS1245 and IS901-flanking region PCR with internal standards. Journal of Microbiological Methods, 64, 333-345. Go to original source... Go to PubMed...
    3. Bougiouklis P, Brellou G, Fragkiadaki E, Iordanidis P, Vlemmas I, Georgopoulou I (2005): Outbreak of avian mycobacteriosis in a flock of two-year-old domestic pigeons (Columba livia f. domestica). Avian Diseases, 49, 442-445. Go to original source... Go to PubMed...
    4. Cho HS, Kim YH, Park NY (2006): Disseminated mycobacteriosis due to Mycobacterium avium in captive Bengal tiger (Panthera tigris). Journal of Veterinary Diagnostic Investigation, 18, 312-314. Go to original source... Go to PubMed...
    5. Dvorska L, Bull TJ, Bartos M, Matlova L, Svastova P, Weston RT, Kintr J, Parmova I, van Soolingen D, Pavlik I (2003): A standardised restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS901 with virulence for birds. Journal of Microbiological Methods, 55, 11-27. Go to original source... Go to PubMed...
    6. Fischer O, Matlova L, Bartl J, Dvorska L, Melicharek I, Pavlik I (2000): Findings of mycobacteria in insectivores and small rodents. Folia Microbiologica, 45, 147-152. Go to original source... Go to PubMed...
    7. Hejlicek K, Treml F (1994): Epizootiology and pathogenesis of avian mycobacteriosis in the pigeon (Columba livia f. Domestica (in Czech with English abstract). Veterinarni Medicina, 39, 615-624.
    8. Hejlicek K, Treml F (1997): Animal sources and ways of spreading Mycobacterium avium (in Czech with English abstract). Epidemiologie, Mikrobiologie, Imunologie, 46, 115-118.
    9. Kul O, Tunica R, Haziroglu R, Diker KS, Karahan S (2005): An outbreak of avian tuberculosis in peafowl (Pavo cristatus) and pheasants (Phasianus colchicus) in a zoological aviary in Turkey. Veterinarni Medicina, 50, 446- 450. http://old.vri.cz/docs/vetmed/50-10-446.pdf Go to original source...
    10. Mijs W, de Haas P, Rossau R, Van der Laan T, Rigouts L, Portaels F, van Soolingen D (2002): Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and 'M. avium subsp. hominissuis' for the human/porcine type of M. avium. International Journal of Systematic and Evolutionary Microbiology, 52, 1505-1518. Go to original source...
    11. Moravkova M, Bartos M, Dvorska-Bartosova L, Beran V, Parmova I, Ocepek M, Pate M, Pavlik I (2007): Genetic variability of Mycobacterium avium subsp. avium of pig isolates. Veterinarni Medicina, 52, 430-436. http://old.vri.cz/docs/vetmed/52-10-430.pdf Go to original source...
    12. Moravkova M, Hlozek P, Beran V, Pavlik I, Preziuso S, Cuteri B, Bartos M (2008): Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues. Research in Veterinary Science, 85, 257-264. Go to original source... Go to PubMed...
    13. Morita Y, Arai M, Nomura O, Maruyama S, Katsube Y (1994): Avian tuberculosis which occurred in an imported pigeon and pathogenicity of the isolates. Journal of Veterinary Medical Science, 56, 585-587. Go to original source... Go to PubMed...
    14. Ocepek M, Pate M, Zolnir-Dovc M, Cvetnic Z (2003): Tuberculosis in cattle caused by IS901+ Mycobacterium avium subsp. avium - a case report. Veterinarni Medicina, 48, 47-50. http://old.vri.cz/docs/vetmed/48-2-47.pdf Go to original source...
    15. Pavlik I, Svastova P, Bartl J, Dvorska L, Rychlik I (2000): Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans and the environment and virulence for poultry. Clinical and Diagnostic Laboratory Immunology, 7, 212-217. Go to original source... Go to PubMed...
    16. Pavlik I, Jahn P, Moravkova M, Matlova L, Treml F, Cizek A, Nesnalova E, Dvorska-Bartosova L, Halouzka R (2008): Lung tuberculosis in a horse caused by Mycobacterium avium subsp. avium of serotype 2: a case report. Veterinarni Medicina, 53, 111-116. http://www.vri.cz/docs/vetmed/53-2-111.pdf Go to original source...
    17. Schrenzel M, Nicolas M, Witte C, Papendick R, Tucker T, Keener L, Sutherland-Smith M, Lamberski N, Orndorff D, Heckard D, Witman P, Mace M, Rimlinger D, Reed S, Rideout B (2008): Molecular epidemiology of Mycobacterium avium subsp. avium and Mycobacterium intracellulare in captive birds. Veterinary Microbiology, 126, 122-131. Go to original source... Go to PubMed...
    18. Shitaye JE, Parmova I, Matlova L, Dvorska L, Horvathova A, Vrbas V, Pavlik I (2006): Mycobacterial and Rhodococcus equi infections in pigs in the Czech Republic between the years 1996 and 2004: the causal factors and distribution of infections in the tissues. Veterinarni Medicina, 51, 497-511. http://old.vri.cz/docs/vetmed/51-11-497.pdf Go to original source...
    19. Shitaye JE, Matlova L, Horvathova A, Moravkova M, Dvorska-Bartosova L, Treml F, Lamka J, Pavlik I (2008a): Mycobacterium avium subsp. avium distribution studied in a naturally infected hen flock and in the environment by culture, serotyping and IS901 RFLP methods. Veterinary Microbiology, 127, 155-164. Go to original source... Go to PubMed...
    20. Shitaye JE, Matlova L, Horvathova A, Moravkova M, Dvorska-Bartosova L, Trcka I, Lamka J, Treml F, Vrbas V, Pavlik I (2008b): Diagnostic testing of different stages of avian tuberculosis in naturally infected hens (Gallus domesticus) by the tuberculin skin agglutination tests, faecal and egg examinations. Veterinarni Medicina, 53, 101-110. http://www.vri.cz/docs/vetmed/53-2-101.pdf Go to original source...
    21. Sussland Z, Hrdinova V (1976): Use of rapid agglutination in the serotyping of the Mycobacterium avium-intracellulare complex (in Czech). Veterinarni Medicina, 21, 209-213.
    22. Thorel M, Huchzermeyer HF, Michel AL (2001): Mycobacterium avium and Mycobacterium intracellulare infection in mammals. Revue Scientifique et Technique de L Office International des Epizooties, 20, 204-218. Go to original source... Go to PubMed...
    23. Wolinsky E, Schaefer WB (1973): Proposed numbering scheme for mycobacterial serotypes by agglutination. International Journal of Systematic Bacteriology, 23, 182-183. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content