Vet Med - Czech, 2013, 58(12):605-608 | DOI: 10.17221/7178-VETMED

Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattleOriginal Paper

P. Kumar, B.L. Jangir, G. Saikumar, R. Somvanshi
Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

The present study was carried out to investigate the involvement of different bovine papillomaviruses in the teat warts of cattle. A total of 11 teat wart samples showing rice grain-like and small, sessile elevated greyish or flesh-like growths were collected from dairy cattle. DNA was extracted from these teat wart samples and PCR and real time PCR techniques were applied using specific primers for BPV-1 and -10 to detect the presence of viral nucleic acid. PCR revealed the presence of viral DNA of BPV-1 and -10 in three and seven samples, respectively. Quantification using real time PCR revealed that the copy numbers of the viral DNA of BPV-1 and -10 DNA varied from 1.12E + 04 to 2.99E + 04 and 3.56E + 02 to 5.23E + 06, respectively. From the present study it can be concluded that BPV-1 and -10 are involved in production of rice grain-like and sessile elevated growths on the teats of cattle.

Keywords: teat wart; bovine papillomavirus; cattle, PCR; real time PCR

Published: December 31, 2013  Show citation

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Kumar P, Jangir BL, Saikumar G, Somvanshi R. Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle. Vet Med - Czech. 2013;58(12):605-608. doi: 10.17221/7178-VETMED.
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