Vet Med - Czech, 2014, 59(9):424-432 | DOI: 10.17221/7682-VETMED

Effects of Bai zhu san (Atractylodis macrocephalae) decoction on cellular immunity and Th1/Th2 cytokine ratio in a Mifepristone-induced murine abortion modelOriginal Paper

M.Y. Geng, F.Z. Yuan, X.D. Wang, X.H. Zhong
Agricultural University of Hebei, Baoding, P.R. China

Chinese herbal medicine has been used as an anti-abortive treatment in farm animals for thousands of years. To investigate the anti-abortive functions and mechanisms of Bai zhu san (BZS) on mifepristone (RU-486)-induced abortion, pregnant mice were allocated to different groups as follows: control group receiving neither RU-486 nor BZS; mice receiving RU-486 only; mice treated with both RU-486 and BZS. The results showed that the concentrations of IL-10 and IL-4 in uterine lysates were significantly higher in mice of the BZS + RU-486 group compared with the mice of the RU-486 group. The IL-10 and IL-4 levels in serum were several folds higher than that in uterus lysates. The IFN-γ concentrations in uterine lysate decreased significantly in mice of the BZS + RU-486 group vs. the RU-486 group. There were no significant differences in IL-2 concentrations between the mice of BZS + RU-486 or RU-486 groups and controls in the uterus and serum. The numbers of CD4+, CD8+T lymphocytes or macrophages in the uterus decreased in the BZS + RU-486 group compared with the RU-486 group. These results indicate that the Chinese herbal formula Bai zhu san inhibits RU-486-induced abortion and modulates the Th1/Th2 cytokine balance at the maternal-foetal interface.

Keywords: Bai zhu san; mifepristone; cytokine; anti-abortive; CD4+, CD8+T cells

Published: September 30, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Geng MY, Yuan FZ, Wang XD, Zhong XH. Effects of Bai zhu san (Atractylodis macrocephalae) decoction on cellular immunity and Th1/Th2 cytokine ratio in a Mifepristone-induced murine abortion model. Vet Med - Czech. 2014;59(9):424-432. doi: 10.17221/7682-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Baines MG, Duglos AJ, de Fougerolles AR, Gendron AL(1996): Immunological prevention of spontaneous early embryo resortion is mediated by non-specific immunostimulation. American Journal of Reproductive Immunology 35, 34-42. Go to original source... Go to PubMed...
    2. Chang JM, Hung LM, Chyan YJ, Cheng CM, Wu RY (2011): Carthamus tinctorius enhances the antitumor activity of dendritic cell vaccines via polarization toward Th1 cytokines and increase of cytotoxic T lymphocytes. Evidence-Based Complementary Alternative Medicine ID 274858, dx.doi.org/10.1093/ecam/nen068 Go to original source...
    3. Chaouat G, Petitbarat M, Bulla R, Dubanchet S, Valdivia K, Ledee N, Steffen T, Jensenius JC, Tedesco F (2009): Early regulators in abortion and implications for a preeclampsia model. Journal Reproductive Immunology 82, 131-140. Go to original source... Go to PubMed...
    4. Chatterjee A, Chattopadhyay S, Bandyopadhyay SK (2011): Biphasic effect of Phyllanthus emblica L. extract on NSAID-induced ulcer: an antioxidative trail weaved with immunomodulatory effect. EvidenceBased Complementary Alternative Medicine 146808. doi: 10.1155/2011/146808. Go to original source... Go to PubMed...
    5. Chiu PR, Lee WT, Chu YT, Lee MS, Jong YJ, Hung CH (2008): Effect of the Chinese herb extract osthol on IL4-induced eotaxin expression in BEAS-2B cells. Pediatrics and Neonatology 49, 135-140. Go to original source... Go to PubMed...
    6. Curry AE, Vogel I, Skogstrand K, Drews C, Schendel DE, Flanders WD, Hougaard DM, Thorsen P (2008): Maternal plasma cytokines in early-and mid-gestation of normal human pregnancy and their association with maternal factors. Journal of Reproductive Immunology 77, 152-160. Go to original source... Go to PubMed...
    7. Davey A (2006): Mifepristone and prostaglandin for termination of pregnancy: contraindications for use, reasons and rationale. Contraception 74, 16-20. Go to original source... Go to PubMed...
    8. Dealtry GB, O'Farrell MK, Fernandez N (2000): The Th2 cytokine environment of the placenta. International Archives of Allergy and Immunology 123, 107-119. Go to original source... Go to PubMed...
    9. Dourado LP, Saldanha JC, Gargiulo DL, Noviello MDL, Brant CC, Reis ML, de Souza RMF, Faria AM, de Souza DD, Cara DC (2010): Role of IL-4 in aversion induced by food allergy in mice. Cell Immunology 262, 62-68. Go to original source... Go to PubMed...
    10. Faas MM, Moes H, Van der Schaaf G, De Leij L, Heineman MJ (2003): Total white blood cell counts and LPS-induced TNF-α production by monocytes of pregnant, pseudopregnant and cyclic rats. Journal of Reproductive Immunology 59, 39-52. Go to original source... Go to PubMed...
    11. Fukui A, Kwak-Kim J, Ntrivalas E, Gilman-Sachs A, Lee S, Beaman K (2008): Intracellular cytokine expression of peripheral blood natural killer cell subsets in women with recurrent spontaneous abortions and implantation failures. Fertility and Sterility 89, 157-165. Go to original source... Go to PubMed...
    12. Hapangama DK, Brown A, Glasier AF, Baird DT (2001): Feasibility of administering mifepristone as a once a month contraceptive pill. Human Reproduction 16, 1145-1150. Go to original source... Go to PubMed...
    13. Hill JA, Polgar K, Anderson DJ (1995): T-helper 1-type immunity to trophoblast in women with recurrent spontaneous abortion. Journal of the American Medical Association 273, 1933-1936. Go to original source... Go to PubMed...
    14. Ikemoto S, Sugimura K, Yoshida N, Yasumoto R, Wada S, Yamamoto K, Kishimoto T (2000): Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines. Urology 55, 951-955. Go to original source... Go to PubMed...
    15. Jacques SM, Qureshi F (1998): Chronic chorioamnionitis: a clinicopathologic and immunohistochemical study. Human Pathology 29, 1457-1461. Go to original source... Go to PubMed...
    16. Jiang SP, Vacchio MS (1998): Multiple mechanisms of peripheral T cell tolerance to the fetal "allograft". Journal of Immunology 160, 3086-3090. Go to original source... Go to PubMed...
    17. Joachim R, Zenclussen AC, Polgar B, Douglas AJ, Fest S, Knackstedt M, Klapp BF, Arck PC (2003): The progesterone derivative dydrogesterone abrogates murine stress-triggered abortion by inducing a Th2 biased local immune response. Steroids 68, 931-940. Go to original source... Go to PubMed...
    18. Kim EH, Shim B, Kang S, Jeong G, Lee JS, Yu YB, Chun M (2009): Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules. Journal of Ethnopharmacology 126, 320-331. Go to original source... Go to PubMed...
    19. Li CQ, He LC (2006): Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR4 receptor component from Atractylodes macrocephala Koidz. Science of China C: Life Science 49, 182-189. Go to original source... Go to PubMed...
    20. Li HX, Sze S, Tong Y, Ng TB (2009): Production of Th1and Th2-dependent cytokines induced by the Chinese medicine herb, Rhodiola algida, on human peripheral blood monocytes. Journal of Ethnopharmacology 123, 257-266. Go to original source... Go to PubMed...
    21. Lin C, Shieh D (1996): The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. American Journal of Chinese Medicine 24, 31-36. Go to original source... Go to PubMed...
    22. Lin HH, Faunce DE, Stacey M, Terajewicz A, Nakamura T, Zhang-Hoover J, Kerley M, Mucenski M, Gordon S, Stein-Streilein J (2005): The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. Journal of Experimental Medicine 201, 1615-1625. Go to original source... Go to PubMed...
    23. Luppi P (2003): How immune mechanisms are affected by pregnancy. Vaccine 21, 3352-3357. Go to original source... Go to PubMed...
    24. Ma AT, Zhong XH, Liu ZM, Shi WY, Du J, Zhai XH, Zhang T, Meng LG (2009): Protective effects of baicalin against bromocriptine induced abortion in mice. American Journal of Chinese Medicine 37, 85-95 Go to original source... Go to PubMed...
    25. Ma AT, Wang XD, Gong XC, Zhao XH, Zhong XH (2012): Protective effect of anti-abortive herbal medicine on embryo implantation and the changes of serum progesterone, IFN-γ and IL-4 in cows after artificial insemination. Journal of Medicinal Plants Research 6, 383-390. Go to original source...
    26. Paradisi R, Maldini-Casadei M, Boni P, Busacchi P, Porcu E, Venturoli S (2003): T-helper 2-cytokine levels in women with threatened abortion. European Journal of Obstetrics and Gynecology and Reproductive Biology 111, 43-49. Go to original source... Go to PubMed...
    27. Quinn HE, Miller C, Ellis JT (2004): The cell-mediated immune response to Neospora caninum during pregnancy in the mouse is associated with a bias towards production of interleukin-4. International Journal of parasitology 34, 723-732. Go to original source... Go to PubMed...
    28. Robertson SA (2007): GM-CSF regulation of embryo development and pregnancy. Cytokine Growth Factor Review 18, 287-298. Go to original source... Go to PubMed...
    29. Saito S (2000): Cytokine network at the feto-maternal interface. Journal of Reproductive Immunology 47, 87-103. Go to original source... Go to PubMed...
    30. Sitruk-Ware R, Spitz IM (2003): Pharmacological properties of mifepristone: toxicology and safety in animal and human studies. Contraception 68, 409-420. Go to original source... Go to PubMed...
    31. Wang XD, Ma AT, Shi WY, Geng MY, Zhong XH, Zhao YT (2011): Quercetin and bornyl acetate regulate T-lymphocyte subsets and IFN-γ/IL-4 ratio in utero in pregnant mice. Evidence-based complementary and alternative medicine 745262, 7 pages. doi:10.1155/2011/745262. Go to original source... Go to PubMed...
    32. Watanabe M, Iwatani Y, Kaneda T, Hidaka Y, Mitsuda N, Morimoto Y, Amino N (1997): Changes in T, B, and NK lymphocyte subsets during and after normal pregnancy. American Journal of Reproductive Immunology 37, 368-377. Go to original source... Go to PubMed...
    33. Wegmann TG, Lin H, Guilbert L, Mosmann TR (1993): Bidirectional cytokine interactions in the maternalfetal relationship: is successful pregnancy a TH2 phenomenon? Immunology Today 14, 353-356. Go to original source... Go to PubMed...
    34. Xie HS, Preast V (2010): Xie's Chinese Veterinary Herbalogy. Blackwell Publishing, Iowa, USA. 324-325.
    35. Yang Q, Shi WY, Zhong XH (2011): Influence of quercetin on transforming growth factor β1 in the uterus following LPS-induced abortion in pregnant mice. Journal of Medicinal Plants Research 5, 3475-3479.
    36. Yeap SK, Omar AR, Ali AM, Ho WY, Beh BK, Alitheen NB (2012): Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. Evidence-based Complementary and Alternative Medicine 786487. DOI: 10.1155/2012/786487 Go to original source... Go to PubMed...
    37. Zenclussen AC (2005): CD4(+)CD25+ T regulatory cells in murine pregnancy. Journal of Reproductive Immunology 65, 101-110. Go to original source... Go to PubMed...
    38. Zenclussen AC, Blois S, Stumpo R, Olmos S, Arias K, Malan BI, Roux ME, Margni RA (2003): Murine abortion is associated with enhanced interleukin-6 levels at the feto-maternal interface. Cytokine 24, 150-160. Go to original source... Go to PubMed...
    39. Zhao JX, Zeng YY, Liu Y (2007): Fetal alloantigen is responsible for the expansion of the CD4(+)CD25(+) regulatory T cell pool during pregnancy. Journal of Reproductive Immunology 75, 71-81. Go to original source... Go to PubMed...
    40. Zhao YT, Bao YZ, Shi WY, Wang XD, Zhong XH (2011a): Protective effects of baicalin on lipopolysaccharide (LPS)-induced implantation failure and the uterine endometrium in mice. African Journal of Pharmacy and Pharmacology 5, 1661-1668. Go to original source...
    41. Zhao YT, Wang XD, Shi WY, Zhong XH (2011b): Antiabortive effect of quercetin and bornyl acetate on macrophages and IL-10 in uterus of mice. African Journal of Biotechnology 10, 8675-8682. Go to original source...
    42. Zhong XH, Zhou ZX, Li TS, Wang EQ, Shi WY, Chu SM (2002): Anti-abortive effect of Radix scutellariae and Rhizoma atractylodis in mice. American Journal of Chinese Medicine 30, 109-117. Go to original source... Go to PubMed...
    43. Zhong XH, Shi WY, Ma AT, Gong XC, Zhai XH, Zhang T, Wang XD (2008): Effects of Radix scutellariae and Rhizoma atractylodis on LPS-induced abortion and the uterine IL-10 contents in mice. American Journal of Chinese Medicine 36, 141-148. Go to original source... Go to PubMed...
    44. Zhou M, Mellor AL (1998): Expanded cohorts of maternal CD8+ T-cells specific for paternal MHC class I accumulate during pregnancy. Journal of Reproductive Immunology 40, 47-62. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content