Vet Med - Czech, 2015, 60(6):309-313 | DOI: 10.17221/8245-VETMED

Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cowsOriginal Paper

G. Turk1, M. Yuksel2, M. Sonmez1, S. Gur1, S. Ozer Kaya1, E. Demirci1
1 Faculty of Veterinary Medicine, Firat University, Elazig, Turkey
2 Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey

It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, HeiferplusTM (HP, in favour of female gender) and BullplusTM (BP, in favour of male gender), increases the birth chance of calves with desired sex ratio by at least 20-25% and pregnancy rates by at least 5-20%. Hence, this study was conducted to investigate the efficacy of HP and BP kits as combined with AI on the pregnancy rates, foetal sex ratios and some reproductive parameters in cows. For this, a total of 200 cows (100 Holsteins and 100 Simmentals) from three to five years old were used. Fifty Holstein and 50 Simmental cows served as controls. The other half of Holstein and Simmental cows was artificially inseminated with frozen-thawed semen treated with HP and BP, respectively. Findings showed that the AI of cows with frozen-thawed semen treated with HP had no significant effect on the pregnancy rate [52.0% (26/50) in HP group; 56.0% (28/50) in control group], female calf ratio [52.0% (13/25) in HP group; 44.4% (12/27) in control group], embryonic death, abortion, stillbirth, twinning and gestation length as compared to the control group. Similarly, AI of cows with frozen-thawed semen treated with BP did not lead to any significant effect on the pregnancy rate [64.0% (32/50) in BP group; 58.0% (29/50) in control group], male calf ratio [53.1% (17/32) in BP group; 39.3% (11/28) in control group] and other reproductive parameters as compared to the control group. In conclusion, HP and BP treatments of semen used in the AI provided only slight, non-significant increases in female (7.6%) and male (13.8%) calf ratios, respectively.

Keywords: Heiferplus; Bullplus; foetal sex; pregnancy rate; cow

Published: June 30, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Turk G, Yuksel M, Sonmez M, Gur S, Ozer Kaya S, Demirci E. Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows. Vet Med - Czech. 2015;60(6):309-313. doi: 10.17221/8245-VETMED.
Download citation

References

  1. Curry E, Pratt SL, Lapin DR, Gibbons JR (2009): Efficacy of a commercially available post-thaw bovine semen sexing kit in both single-ovulating and hyperstimulated cows. Animal Reproduction Science 116, 376-380. Go to original source... Go to PubMed...
  2. Gerard O, Salas-Cortes L, Sellem E, Marquant-Leguienne B, Clement L, Humblot P (2008): In vitro assessment of the effectiveness of a commercially available post-thaw bovine semen sexing kit on semen quality parameters, in vitro fertilizing ability and sex-ratio deviation. In: Proceedings of 24th Annual Meeting A.E.T.E. (Association Europeenne de Transfert Embryonnaire), Pau, France, 12-13 September 2008, 154 pp.
  3. Reidhead P (2007): $6.50/Vial: HeiferPlusTM dramatically boosts heifer calf numbers. The Milkweed 6-7. http://www.emlabgenetics.com/Documents/HeiferPlus_story_from_The_Milkweed.pdf (accessed 05 September 2014).
  4. Sassone F, Guaricci AC, Lacalandra GM, Minoia R (2009): Effect of HeiferplusTM on bull semen parameters accessed by CASA analyzer. In: Proceedings of 25th Annual Meeting A.E.T.E. (Association Europeenne de Transfert Embryonnaire), Poznan, Poland, 11-12 September 2009, 252 pp.
  5. Seidel GE Jr (1999): Sexing mammalian sperm and embryosstate of the art. Journal of Reproduction and Fertility 54 (Suppl), 475-485.
  6. Seidel GE Jr (2007): Overview of sexing sperm. Theriogenology 68, 443-446. Go to original source... Go to PubMed...
  7. Seidel GE Jr, Johnson LA (1999): Sexing mammalian spermoverview. Theriogenology 52, 1267-1272. Go to original source... Go to PubMed...
  8. Williams T (2007): The use of HeiferPLUS in superovulated heifers. http://www.emlabgenetics.com/heiferplusdata (accessed 05 September 2014).

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.