Vet Med - Czech, 2016, 61(8):421-427 | DOI: 10.17221/138/2015-VETMED

Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistryOriginal Paper

M. Ozkaraca1, S. Ceribasi2, A.O. Ceribasi2, A. Kilic3, S. Altun1, S. Comakli1, H. Ongor2
1 Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey
2 Faculty of Veterinary Medicine, Firat University, Elazig, Turkey
3 Sivrice Vocational High School, Firat University, Elazig, Turkey

This study was aimed at detection of Coxiella burnetii in bovine foetuses using polymerase chain reaction (PCR) and immunohistochemistry (IHC) and at an estimation of its frequency in Eastern Turkey. Stamp, Giemsa, and Gimenez stains were used in addition to PCR and IHC to determine the presence of C. burnetii in samples from 70 bovine foetuses. While the staining methods did not detect the agent by direct visualisation of C. burnetii on smears, PCR and IHC identified its presence in two of the foetuses. The distribution of antigens in these two foetuses was, in decreasing order of concentration, in the spleen, the thymus, the lungs, the liver, and the kidneys. We conclude that C. burnetii diagnosis in bovine foetuses can be reliably performed using PCR and IHC. In addition, the frequency of 1.42% of C. burnetii positivity in bovine foetuses reported here was the first time that the presence of this agent was determined in Eastern Turkey.

Keywords: Q fever; diagnostic method; IHC; cow; Eastern Turkey

Published: August 31, 2016  Show citation

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Ozkaraca M, Ceribasi S, Ceribasi AO, Kilic A, Altun S, Comakli S, Ongor H. Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry. Vet Med - Czech. 2016;61(8):421-427. doi: 10.17221/138/2015-VETMED.
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    References

    1. Adesiyun AA, Jagun AG, Tekdek LB (1984): Coxiella burnetii antibodies in some Nigerian dairy cows and their suckling calves. International Journal of Zoonoses 11, 155-160.
    2. Baumgartner W, Bachmann S (1992): Histological and immunocytochemical characterization of Coxiella burnetiiassociated lesions in the murine uterus and placenta. Infection and Immunity 60, 5232-5241. Go to original source... Go to PubMed...
    3. Behymer DE, Biberstein EL, Riemann HP, Franti CE, Sawyer M, Ruppanner R, Crenshaw GL (1976): Q fever (Coxiella burnetii) investigations in dairy cattle: challenge of immunity after vaccination. American Journal of Veterinary Research 37, 631-634.
    4. Behymer DE, Ruppanner R, Brooks D, Williams JC, Franti CE (1985): Enzyme immunoassay for surveillance of Q fever. American Journal of Veterinary Research 46, 2413-2417.
    5. Berri M, Laroucau K, Rodolakis A (2000): The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Veterinary Microbiology 72, 285-293. Go to original source... Go to PubMed...
    6. Bildfell RJ, Thomson GW, Haines DM, McEwen BJ, Smart N (2000): Coxiella burnetii infection is associated with placentitis in cases of bovine abortion. Journal of Veterinary Diagnostic Investigation 12, 419-425. Go to original source... Go to PubMed...
    7. Borriello G, Galiero G (2012): Coxiella burnetii. In: LorenzoMorales J (ed.): Zoonosis. InTech, Rijeka. 65-88. Go to original source...
    8. Chanton-Greutmann H, Thoma R, Corboz L, Borel N, Pospischil A (2002): Abortion in small ruminants in Switzerland: investigations during two lambing seasons (1996-1998) with special regard to chlamydial abortions. Schweizer Archiv fur Tierheilkunde 144, 483-492. Go to original source... Go to PubMed...
    9. Christie AB (1974): Q fever. In: Christie AB (ed.): Infectious Diseases, Epidemiology and Clinical Practice. Churchill Livingston, Edinburgh. 876-891.
    10. Clemente L, Barahona MJ, Andrade MF, Botelho A (2009): Diagnosis by PCR of Coxiella burnetii in aborted fetuses of domestic ruminants in Portugal. Veterinary Record 164, 373-374. Go to original source... Go to PubMed...
    11. Dogru AK, Yildirim M, Unal N, Gazyagci S (2010): The relationship of Coxiella burnetii seropositivity between farm animals and their owners: A pilot study. Journal of Animal and Veterinary Advances 9, 1625-1629. Go to original source...
    12. Gimenez DF (1964): Staining rickettsiae in yolk sac cultures. Stain Technology 30, 135-137. Go to original source... Go to PubMed...
    13. Gunaydin E, Mustak HK, Sarieyyupoglu B, Ata Z (2015): PCR detection of Coxiella burnetii in fetal abomasal contents of ruminants. Kafkas Universitesi Veteriner Fakultesi Dergisi 21, 69-73. Go to original source...
    14. Hazlett MJ, McDowall R, DeLay J, Stalker M, McEwen B, Van Dreumel T, Spinato M, Binnington B, Slavic D, Carman S, Cai HY (2013): A prospective study of sheep and goat abortion using real-time polymerase chain reaction and cut point estimation shows Coxiella burnetii and Chlamydophila abortus infection concurrently with other major pathogens. Journal of Veterinary Diagnostic Investigation 25, 359-368. Go to original source... Go to PubMed...
    15. Hoover AT, Vodkin MH, Williams JC (1992): A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. Journal of Bacteriology 174, 5540- 5548. Go to original source... Go to PubMed...
    16. Houwers DJ, Richardus JH (1987): Infections with Coxiella burnetii in man and animals in the Netherlands. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene 267, 30-36. Go to original source... Go to PubMed...
    17. Jensen TK, Montgomery DL, Jaeger PT, Lindhardt T, Agerholm JS, Bille-Hansen V, Boye M (2007): Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetii in placentas from ruminant abortions. Acta Pathologica, Microbiologica, et Immunologica Scandinavica 115, 347-353. Go to original source... Go to PubMed...
    18. Kim SG, Kim EH, Lafferty CJ, Dubovi E (2005): Coxiella burnetii in bulk tank milk samples, United States. Emerging Infectious Diseases 11, 619-621. Go to original source... Go to PubMed...
    19. Kirkan S, Kaya O, Tekbiyik S, Parin U (2008): Detection of Coxiella burnetii in cattle by PCR. Turkish Journal of Veterinary and Animal Sciences 32, 215-220.
    20. Marrie TJ, Van Buren J, Fraser J, Haldane EV, Faulkner RS, Williams JC, Kwan C (1985): Seroepidemiology of Q fever among domestic animals in Nova Scotia. American Journal of Public Health 75, 763-766. Go to original source... Go to PubMed...
    21. Maurin M, Raoult D (1999): Q fever. Clinical Microbiology Reviews 12, 518-553. Go to original source... Go to PubMed...
    22. McQuiston JH, Childs JE (2002): Q fever in humans and animals in the United States. Vector-Borne Zoonotic Diseases 2, 179-191. Go to original source... Go to PubMed...
    23. Moeller RB (2001): Causes of caprine abortion: diagnostic assessment of 211 cases (1991-1998). Journal of Veterinary Diagnostic Investigation 13, 265-270. Go to original source... Go to PubMed...
    24. Muskens J, Wouda W, Von Bannisseht-Wijsmuller T, Van Maanen C (2012): Prevalence of Coxiella burnetii infections in aborted fetuses and stillborn calves. Veterinary Record 170, 260. Go to original source... Go to PubMed...
    25. Ongor H, Cetinkaya B, Karahan M, Acik MN, Bulut H, Muz A (2004): Detection of Coxiella burnetii by immunomagnetic separation-PCR in the milk of sheep in Turkey. Veterinary Record 154, 570-572. Go to original source... Go to PubMed...
    26. Parisi A, Fraccalvieri R, Cafiero M, Miccolupo A, Padalino I, Montagna C, Capuano F, Sottili R (2006): Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Veterinary Microbiology 118, 101-106. Go to original source... Go to PubMed...
    27. Plagemann O (1989): The most frequent infectious causes of abortion in sheep in north Bavaria with special reference to Chlamydia and Salmonella infections. Tierarzliche Praxis 17, 145-148.
    28. Plommet M, Capponi M, Gestin J, Renoux G (1973): Fievre Q experimentale des bovines. Annales de Recherches Veterinaires 4, 325-346.
    29. Rady M, Glavits R, Nagy G (1985): Demonstration in Hungary of Q fever associated with abortions in cattle and sheep. Acta Veterinaria Hungarica 33, 169-176.
    30. Rodolakis A, Berri M, Hechard C, Caudron C, Souria UA, Bodier CC, Blanchard B, Camuset P, Devillechaise P, Natorp JC, Vadet JP, Arri Cau-Bouvery N (2007): Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds. Journal of Dairy Science 90, 5352-5360. Go to original source... Go to PubMed...
    31. Sanchez J, Souriau A, Buendia AJ, Arricau-Bouvery N, Martinez CM, Salinas J, Rodolakis A, Navarro JA (2006): Experimental Coxiella burnetii infection in pregnant goats: a histopathological and immunohistochemical study. Journal of Comparative Pathology 135, 108-115. Go to original source... Go to PubMed...
    32. Seyitoglu S, Ozkurt Z, Dinler U, Okumus B (2006): The seroprevalence of Coxiellosis in farmers and cattle in Erzurum district in Turkey. Turkish Journal of Veterinary and Animal Sciences 30, 71-75.
    33. Szeredi L, Janosi S, Tenk M, Tekes L, Bozso M, Deim Z, Molnar T (2006): Epidemiological and pathological study on the causes of abortion in sheep and goats in Hungary (1998-2005). Acta Veterinaria Hungarica 54, 503-515. Go to original source... Go to PubMed...

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