Veterinární medicína, 2016 (vol. 61), issue 8
Effects of erythromycin on milk yield, rumen contraction rate and blood beta-hydroxybutyrate concentration in dairy cows after laparoscopic reposition of the left displaced abomasumOriginal Paper
M. Freick, J. Weber, O. Passarge, S.D. Kevork
Vet Med - Czech, 2016, 61(8):413-420 | DOI: 10.17221/20/2016-VETMED 
The objective of this study was to evaluate the beneficial effects of erythromycin (Emy), a prokinetic drug active at the level of the abomasum, duodenum and proximal duodenum, in dairy cows undergoing a laparoscopic repositioning of left abomasal displacement (LAD). A total of 71 German Holstein cows were randomly assigned into three groups. Cows of the group Emy1 received erythromycin once preoperatively (10 mg/kg; intramuscularly). Animals of the group Emy3 were additionally treated twice after surgery at intervals of 24 h each (10 mg/kg; intramuscularly), while cows in the control group did not receive erythromycin. The milk yield of the patients...
Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistryOriginal Paper
M. Ozkaraca, S. Ceribasi, A.O. Ceribasi, A. Kilic, S. Altun, S. Comakli, H. Ongor
Vet Med - Czech, 2016, 61(8):421-427 | DOI: 10.17221/138/2015-VETMED
This study was aimed at detection of Coxiella burnetii in bovine foetuses using polymerase chain reaction (PCR) and immunohistochemistry (IHC) and at an estimation of its frequency in Eastern Turkey. Stamp, Giemsa, and Gimenez stains were used in addition to PCR and IHC to determine the presence of C. burnetii in samples from 70 bovine foetuses. While the staining methods did not detect the agent by direct visualisation of C. burnetii on smears, PCR and IHC identified its presence in two of the foetuses. The distribution of antigens in these two foetuses was, in decreasing order of concentration, in the spleen, the thymus,...
Effect of linseed and the combination of conjugated linoleic acid and linseed on the quality and oxidative stability of pig meat and subcutaneous fatOriginal Paper
E. Vaclavkova, Z. Volek, J. Belkova, D. Duskova, M. Czauderna, M. Marounek
Vet Med - Czech, 2016, 61(8):428-435 | DOI: 10.17221/117/2015-VETMED
The aim of this experiment was to test the hypothesis that conjugated linoleic acid (CLA) in diets of finishing pigs fed linseed can improve the quality and oxidative stability of meat and subcutaneous fat. Twenty-four Prestice Black-Pied pigs (barrows and gilts) were divided into three groups and were fed a basal diet and diets supplemented with ground linseed (70 g/kg), or linseed combined with conjugated linoleic acid (20 g CLA-oil/kg). The trial duration was 53 days. Measurements included slaughter and meat quality parameters, oxidative stability determination, and fatty acid profile of meat and subcutaneous fat. The experimental data were analysed...
Radiographic findings in sheep with abomasal phytobezoariasisOriginal Paper
S.M. Hashemiasl, S. Azizi, D. Torkamani
Vet Med - Czech, 2016, 61(8):436-442 | DOI: 10.17221/9/2016-VETMED
The purpose of this study was to characterise the radiographic appearance of abomasal phytobezoars in sheep as well as to evaluate the utility of abdominal radiography to identify them. Twenty-seven fat-tailed Herrick sheep with a clinical suspicion of abomasal impaction were examined radiographically. Abdominal survey radiographs in right lateral recumbency were taken. Abomasal phytobezoars (AP) were seen in abdominal survey radiographs in 25/27 sheep (92%). Their radiographic survey appearance was round-to-oval masses with radiopaque margins and radiopacity similar to the ingesta centrally. An additional gastrographic barium study was performed in...
Freezing and storage of leukodepleted erythrocyte suspensionsOriginal Paper
D.A. Bala, E. Eraslan, I. Akyazi, E.E. Ekiz, M. Ozcan, U. Cotelioglu, M. Arslan
Vet Med - Czech, 2016, 61(8):443-448 | DOI: 10.17221/209/2015-VETMED
Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were...
Presence of Arcobacter species in pet cats and dogs in the Czech RepublicOriginal Paper
M. Pejchalova, S. Zabcikova, L. Silhova, D. Silha, I. Brozkova, M. Haslova
Vet Med - Czech, 2016, 61(8):449-455 | DOI: 10.17221/273/2015-VETMED
This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat....
Levels of fatty acids in the whole body of hens and cocks of the Cobb 500 and Ross 308 hybrid combinations at the end of the fattening periodOriginal Paper
P. Suchy, E. Strakova, I. Herzig
Vet Med - Czech, 2016, 61(8):456-463 | DOI: 10.17221/28/2016-VETMED
In this study, we determined the levels of individual fatty acids in the whole chicken body, as well as dry matter, nitrogenic substances and fat in Cobb 500 and Ross 308 chickens after 40 days of fattening. The highest level of all fatty acids (FA), regardless of the hybrid combination and sex of the chickens, was determined for oleic/elaidic acid, followed by palmitic, linoleic/linolelaidic, palmitoleic and stearic acids. In Cobb 500 cocks, higher values (P ≤ 0.05; P ≤ 0.01) were found for all saturated fatty acids (SaFA) compared to hens. With the exception of γ-linoleic acid, the levels of n-6 FA measured in cocks...
The first finding of Hassall's corpuscles in the thymi of cultured sturgeonsOriginal Paper
E. Salkova, M. Flajshans
Vet Med - Czech, 2016, 61(8):464-466 | DOI: 10.17221/247/2015-VETMED
Histological examination of thymi from juvenile Acipenser ruthenus and Acipenser brevirostrum after haematoxylin and eosin staining revealed the presence of Hassall's corpuscles, in a scattered distribution within the thymic medulla in two of seven, and one of nine examined specimens, respectively. Morphologically, the Hassall's corpuscles were of the juvenile and premature type and no degenerative changes were seen.
Successful response to imatinib in two dogs with inoperable grade III infiltrating mast cell tumours: a case reportCase Report
J.H. Kim, H.J. Kim, D.H. Kim, J.H. Yim, S.J. Lee, K.H. Park, H.Y. Yoon
Vet Med - Czech, 2016, 61(8):467-473 | DOI: 10.17221/87/2016-VETMED
Two dogs were presented owing to sudden rapid growth of cutaneous masses greater than 10 cm on the neck and axillary region, respectively. Based on history and results of physical examinations, blood work, fine needle aspiration, histopathological examination, and computed tomography, inoperable grade III infiltrating mast cell tumours were diagnosed. After the initiation of imatinib treatment, the masses markedly shrank and became undetectable within 10 days in both dogs, although none of the tumour specimens showed evidence of mutations in sequencing of c-kit exons 8 and 11. These results suggest that imatinib could be a therapeutic option...