Vet Med - Czech, 2017, 62(6):308-314 | DOI: 10.17221/125/2015-VETMED

The efficacy of a combination of azithromycin and toltrazuril for the treatment of calves naturally infected with cryptosporidiosis: a randomised, double-blind, placebo-controlled comparative clinical trialOriginal Paper

B.B. Yagci*,1, N. Ocal1, S. Yasa Duru1, M. Akyol2
1 Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey
2 Department of Biostatistics, Yildirim Beyazit University, Ankara, Turkey

Cryptosporidium spp. are important emerging pathogens that can cause infections in humans, especially in immunosuppressed patients. Treatment of diseased calves that shed the infectious stage of the protozoon is critically important to prevent contamination by Cryptosporidium spp. oocysts. The objective of this study was to determine if a combination of azithromycin and toltrazuril provides a better treatment option for calf cryptosporidiosis compared to the use of either agent alone. A total of 55 Holstein calves with cryptosporidiosis were randomly assigned into four groups. Group A (n = 15) received azithromycin at a dose of 20 mg/kg/day per os for six days, group T (n = 15) received toltrazuril at a dose of 20 mg/kg per os every other day on a total of three occasions, and group AT (n = 15) received the combination of both drugs. The control group (n = 10) received purified water. A randomised, placebo-controlled, double-blinded clinical study was designed. The number of oocysts in faeces and clinical parameters were followed daily. Selected haematological and biochemical parameters were measured at the beginning and end of the study. The calves receiving the combination of azithromycin and toltrazuril exhibited a better clinical score as well as the lowest number of oocysts at all time-points. In conclusion, the combination of azithromycin and toltrazuril promotes rapid clinical recovery in calves infected with cryptosporidiosis and stops oocyst shedding. Thus, the combination of azithromycin and toltrazuril is an effective alternative treatment option for calf cryptosporidiosis.

Keywords: neonatal; cattle; diarrhoea; apicomplexan; therapy

Published: June 30, 2017  Show citation

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Yagci BB, Ocal N, Yasa Duru S, Akyol M. The efficacy of a combination of azithromycin and toltrazuril for the treatment of calves naturally infected with cryptosporidiosis: a randomised, double-blind, placebo-controlled comparative clinical trial. Vet Med - Czech. 2017;62(6):308-314. doi: 10.17221/125/2015-VETMED.
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    References

    1. Azami M (2007): Prevalence of cryptosporidium infection in cattle in Isfahan, Iran. Journal of Eukaryotic Microbiology 54, 100-102. Go to original source... Go to PubMed...
    2. Baba T, Yamashita N, Kodama H, Mukamoto M, Asada M, Nakamoto K, Nose Y, McGruder ED (1998): Effect of tylosin tartrate on humoral immune responses in chickens. Zentralblatt fur Veterinarmedizin Reihe B 45, 279-286. Go to original source... Go to PubMed...
    3. Benson JE, Ensley SM, Carson TL, Halbur PG, Janke BH, Quinn WJ (1998): Lasalocid toxicosis in neonatal calves. Journal of Veterinary Diagnostic Investigation 10, 210- 214. Go to original source... Go to PubMed...
    4. Castro-Hermida JA, Gonzales-Losada Y, Freire-Santos F, Mezo-Menendez M, Ares-Mazas E (2001): Evaluation of β-cyclodextrin against natural infections of cryptosporidiosis in calves. Veterinary parasitology 101, 85-89. Go to original source... Go to PubMed...
    5. Castro-Hermida JA, Gonzales-Losada YA, Ares-Mazas E (2002): Prevalence of and risk factors involved in the spread of neonatal bovine cryptosporidiosis in Galicia (NW Spain). Veterinary Parasitology 106, 1-10. Go to original source... Go to PubMed...
    6. Current WL, Garsia LS (1991): Cryptosporidiosis. Clinical Microbiology Reviews 4, 325-358. Go to original source... Go to PubMed...
    7. Cuteri V, Nisoli L, Preziuso S, Attili AR, Guerra C, Lulla D, Traldi G (2005): Application of a new therapeutic protocol against Neospora caninum-induced abortion in cattle: a field study. Journal of Animal and Veterinary Advances 4, 510-514.
    8. Darius AK, Mehlhorn H, Heydorn AO (2004): Effects of toltrazuril and ponazuril on Hammondia heydorni (syn. Neospora caninum) infections in mice. Parasitology Research 92, 520-522. Go to original source... Go to PubMed...
    9. Davis JL, Gardner SY, Jones SL, Schwabenton BA, Papich MG (2002): Pharmacokinetics of azithromycin in foals after i.v. and oral dose and disposition into phagocytes. Journal of Veterinary Pharmacology and Therapeutics 25, 99-104. Go to original source... Go to PubMed...
    10. Degerli K, Kilimcioglu AA, Kurt O, Tamay AT, Ozbilgin A (2003): Efficacy of azithromycin in a murine toxoplasmosis model, employing a Toxoplasma gondii strain from Turkey. Acta Tropica 88, 45-50. Go to original source... Go to PubMed...
    11. Dirikolu L, Yohn R, Garrett F, Chakkath T, Ferguson DC (2009): Detection, quantification and pharmacokinetics of toltrazuril sulfone (Ponazuril®) in cattle. Journal of Veterinary Pharmacology and Therapeutics 32, 280-288. Go to original source... Go to PubMed...
    12. Elitok B, Elitok MO, Pulat H (2005): Efficacy of azithromycin dihydrate in treatment of cryptosporidiosis in naturally infected dairy calves. Journal of Veterinary Internal Medicine 19, 590-593. Go to original source...
    13. Epe C, von Samson-Himmelstjerna G, Wirtherle N, von der Heyden V, Welz C, Beening J, Radeloff I, Hellmann K, Schnieder T, Krieger K (2005): Efficacy of toltrazuril as a metaphylactic and therapeutic treatment of coccidiosis in first-year grazing calves. Parasitology Research 97, 127-133. Go to original source... Go to PubMed...
    14. Fahey T (2003): Cryptosporidiosis. Primary Care Update for OB/GYNS 10, 75-80. Go to original source...
    15. Gottstein B, Eperon S, Dai WJ, Cannas A, Hemphill A, Greif G (2001): Efficacy of toltrazuril and panozuril against experimental Neospora caninum infection in mice. Parasitology Research 87, 43-48. Go to original source... Go to PubMed...
    16. Harp JA, Jardon P, Atwill ER, Zylstra M, Checel S, Goff JP, De Simone C (1996): Field testing of prophylactic measures against Cryptosporidium parvum infection in calves in a California dairy herd. American Journal of Veterinary Research 57, 1586-1588. Go to original source...
    17. Joachim A, Krull T, Schwarzkopf J, Daugschies A (2003): Prevalence and control of bovine cryptosporidiosis in German dairy herds. Veterinary Parasitology 112, 277-288. Go to original source... Go to PubMed...
    18. Klein P, Kleinova T, Volek Z, Simunek J (2008): Effect of Cryptosporidium parvum infection on the absorbtive capacity and paracellular permeability of the small intestine in neonatal calves. Veterinary Parasitology 152, 53-59. Go to original source... Go to PubMed...
    19. Lefay D, Naciri M, Poirier P, Chermette R (2001): Efficacy of halofuginone lactate in the prevention of cryptosporidiosis in suckling calves. Veterinary Record 148, 108-112. Go to original source... Go to PubMed...
    20. Mohri M, Sharifi K, Eidi S (2007): Hematology and serum biochemistry of Holstein dairy calves: Age related changes and comparison with blood composition in adults. Research in Veterinary Science 83, 30-39. Go to original source... Go to PubMed...
    21. Mundt HC, Bangoura B, Mengel H, Keidel J, Daugschies A (2005): Control of clinical coccidiosis of calves due to Eimeria bovis and Eimeria zuernii with toltrazuril under field conditions. Parasitology Research 97, 134-142. Go to original source... Go to PubMed...
    22. O'Handley RM, Cockwill C, McAllister TA, Jelinski M, Morck DW, Olson ME (1999): Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea. Journal of the American Veterinary Medical Association 214, 391-396. Go to original source...
    23. Ocal N, Yagci BB, Yasa Duru S, Kul O (2007): Toltrazuril treatment for acute clinical coccidiosis in hair goat kids: clinical, pathological, haematologic and biochemical findings. Medycyna Weterynaryjna 63, 805-809.
    24. Rehg JE (1991): Activity of azithromycin against cryptosporidia in immunosuppressed rats. Journal of Infectious Diseases 163, 1293-1296. Go to original source... Go to PubMed...
    25. Sahal M, Kurtdede A, Borku MK, Unsuren H, Imren HY, Ozlem MB, Kalinbacak A, Tanyel B (1994): Treatment of newborn diarrheic calves with sodium bicarbonate and saline solution with respect to clinical signs and acid-base balance (in Turkish). Ankara Universitesi Veteriner Fakultesi Dergisi (Veterinary Journal of Ankara University) 41, 509-525.
    26. Tzipori S, Smith M, Halpin C, Angus KW, Sherwood D, Campbell I (1982): Experimental cryptosporidiosis in calves: Clinical manifestations and pathological findings. Veterinary Record 112, 116-120. Go to original source... Go to PubMed...
    27. Uip DE, Lima ALL, Amato VS, Boulos M, Neto VA, Bem David D (1998): Roxithromycin treatment for diarrhea caused by Cryptosporidium spp. in patients with AIDS. The Journal of Antimicrobial Chemotherapy 41, 93-97. Go to original source... Go to PubMed...
    28. Vargas SL, Shenep JL, Flynn PM, Pui CH, Santana VM, Hughes WT (1993): Azithromycin for treatment of severe Cryptosporidium diarrhea in two children with cancer. The Journal of Pediatrics 123, 154-156. Go to original source... Go to PubMed...

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