Vet Med - Czech, 2019, 64(4):178-183 | DOI: 10.17221/20/2018-VETMED

Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitroOriginal Paper

Yancheng Zhou1, †, Fei Liao2, †, Jiahua Weng1, Quan Mo1, Ruiguang Xu1, Yong Zhang1, Zhihua Ren1, Zhijun Zhong1, Zhicai Zuo1, Guangneng Peng1, Junliang Deng1, Cheng Tang3, Yanchun Hu*,1
1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China
2 Animal Epidemic Disease Control Center of Qiandongnan Prefectural, Kaili, China
3 College of Life Science and Technology, Southwest University for Nationalities, Chengdu, China

Plant-based natural products represent an alternative to chemical compounds for the control of mites in veterinary medicine. Here, the essential oil of Elsholtzia densa (E. densa) Benth was extracted using hydrodistillation at a rate of 1.2%. The chemical composition of the essential oil was determined by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS analysis indicated that the principal compounds in the volatile oil of the sample were 4-Pyridinol (28.16%) and thymol (26.58%). The acaricidal activity of E. densa oil against Sarcoptes scabiei (S. scabiei) was tested in vitro. Toxicity test data were analysed using a complementary log-log (CLL) model. The E. densa oil was prepared in five concentrations by dilution with liquid paraffin (1, 2, 4, 8 and 16 mg/ml) and exhibited strong toxicity against S. scabiei with LT50 values of 16.637, 5.075, 2.884, 1.184 and 0.760 h, respectively. The LC50 values were 7.678, 4.623, 2.543, 1.502, 1.298 and 0.981 mg/ml for S. scabiei at 1, 2, 4, 8, 16 and 24 h, respectively. Compared to the control, the essential oil showed significant effects against S. scabiei in vitro. At 16 mg/ml, E. densa oil was found to kill all mites within a 16-h period. The results indicate that E. densa oil possesses potential acaricidal activity in vitro and may be exploited as a novel drug for the effective control of S. scabiei.

Keywords: 4-Pyridinol; thymol; rabbit; botanical acaricides; degradation

Published: April 30, 2019  Show citation

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Zhou Y, Liao F, Weng J, Mo Q, Xu R, Zhang Y, et al.. Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitro. Vet Med - Czech. 2019;64(4):178-183. doi: 10.17221/20/2018-VETMED.
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    References

    1. Abdel-Shafy S, Nasr SM, Abdel-Rahman HH, Habeeb SM (2011): Effect of various levels of dietary Jatropha curcas seed meal on rabbits infested by the adult ticks of Hyalomma marginatum marginatum I. Animal performance, anti-tick feeding and haemogram. Tropical Animal Health and Production 43, 347-357. Go to original source... Go to PubMed...
    2. Alasaad S, Schuster RK, Gakuya F, Theneyan M, Jowers MJ, Maione S, Min AM, Soriguer RC, Rossi L (2012): Applicability of molecular markers to determine parasitic infection origins in the animal trade: a case study from Sarcoptes mites in wildebeest. Forensic Science, Medicine, and Pathology 8, 280-284. Go to original source... Go to PubMed...
    3. Benelli G, Pavela R, Canale A, Mehlhorn H (2016): Tick repellents and acaricides of botanical origin: a green roadmap to control tick-borne diseases? Parasitology Research 115, 2545-2560. Go to original source... Go to PubMed...
    4. Chen ZW, Wen-Man WU, Liu H, Wan Y, Luo YM (2011): Review of chemical constituents of medicinal herbs Herba Moslae. Chinese Journal of Experimental Traditional Medical Formulae 17, 160-164.
    5. da Silva Mendes A, Daemon E, de Oliveira Monteiro CM, Maturano R, Brito FC, Massoni T (2011): Acaricidal activity of thymol on larvae and nymphs of Amblyomma cajennense (Acari: Ixodidae). Veterinary Parasitology 183, 136-139. Go to original source... Go to PubMed...
    6. Daemon E, Maturano R, de Oliveira Monteiro CM, Goldner MS, Massoni T (2012): Acaricidal activity of hydroethanolic formulations of thymol against Rhipicephalus sanguineus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Veterinary Parasitology 186, 542-545. Go to original source... Go to PubMed...
    7. Deng Y, Shi D, Yin Z, Guo J, Jia R, Xu J, Song X, Lv C, Fan Q, Liang X (2012): Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro. Experimental Parasitology 130, 475-477. Go to original source... Go to PubMed...
    8. Ebadollahi A, Sendi J, Aliakbar A, Razmjou J (2015): Acaricidal activities of essential oils from Satureja hortensis (L.) and Teucrium polium (L.) against the two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Egyptian Journal of Biological Pest Control 25, 171-176.
    9. Esteves Filho AB, Oliveira JVD, Matos CHC (2013): Residual efficacy of synthetic acaricides and natural products for Tetranychus urticae Koch on cotton. Revista Brasileira De Ciencias Agrarias 8, 583-586. Go to original source...
    10. Gauthami M, Srinivasan N, Goud NM, Boopalan K, Thirumurugan K (2015): Synthesis of silver nanoparticles using Cinnamomum zeylanicum bark extract and its antioxidant activity. Nanoscience & Nanotechnology-Asia 5, 2-7. Go to original source...
    11. Khan M, Ganie SA, Wani IH, Ganai BA, Masood A, Zargar MA, Malik AH, Hamid R (2012): Free radical scavenging activity of Elsholtzia densa. Journal of Acupuncture & Meridian Studies 5, 104-111. Go to original source... Go to PubMed...
    12. Koul O, Walia S, Dhaliwal G (2008): Essential oils as green pesticides: potential and constraints. Biopesticides International 4, 63-84.
    13. Krawczak FDS, Buzatti A, Pivoto FL, Sangioni LA, Vogel FSF, Botton SDA, Zanetti GD, Manfron MP (2011): Acaricide activity of leaves extracts of Sambucus australis Schltdl. (Caprifoliaceae) at 2% on engorged females of Rhipicephalus (Boophilus) microplus. Ciencia Rural 41, 2159-2163. Go to original source...
    14. Liao F, Hu YC, Tan H, Wu L, Wang Y, Huang Y, Mo Q, Wei YH (2014): Acaricidal activity of 9-oxo-10,11-dehydroageraphorone extracted from Eupatorium adenophorum in vitro. Experimental Parasitology 140, 8-11. Go to original source... Go to PubMed...
    15. Liu G, Wang H, Zhou BH, Song JC (2006): GC-MS analysis of essential oils from elsholtzia ciliata. Chinese Journal of Experimental Traditional Medical Formulae 12, 18-21.
    16. Liu Y, Jian-Yong SI, Cao L, Jia XG (2012): Chemical composition, antimicrobial and antiviral activities of the essential oil of Elsholtzia densa Benth. Natural Product Research & Development 24, 1070-1074.
    17. Martinez-Velazquez M, Rosario-Cruz R, Castillo-Herrera G, Flores-Fernandez J, Alvarez A, Lugo-Cervantes E (2011): Acaricidal effect of essential oils from Lippia graveolens (Lamiales: Verbenaceae), Rosmarinus officinalis (Lamiales: Lamiaceae), and Allium sativum (Liliales: Liliaceae) against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Journal of Medical Entomology 48, 822-827. Go to original source... Go to PubMed...
    18. Sarkar P, Talukdar B, Somchoudhury A (2009): Relative efficacy of diafenthiuron (50 WP) alone and with two different adjuvants against adult and egg stages of tea red spider mite, Oligonychus coffeae (Nietner) under laboratory condition. Journal of Entomological Research 33, 109-112.
    19. Scoralik MG, Daemon E, De Oliveira Monteiro CM, Maturano R (2012): Enhancing the acaricide effect of thymol on larvae of the cattle tick Rhipicephalus microplus (Acari: Ixodidae) by solubilization in ethanol. Parasitology Research 110, 645-648. Go to original source... Go to PubMed...
    20. Seddiek SA, Khater HF, El-Shorbagy MM, Ali AM (2013): The acaricidal efficacy of aqueous neem extract and ivermectin against Sarcoptes scabiei var. cuniculi in experimentally infested rabbits. Parasitology Research 112, 2319-2330. Go to original source... Go to PubMed...
    21. Sharma D, Saxena V, Sanil N, Singh N (1997): Evaluation of oil of Cedrus deodara and benzyl benzoate in sarcoptic mange in sheep. Small Ruminant Research 26, 81-85. Go to original source...
    22. Stara J, Nesvorna M, Hubert J (2011): The toxicity of selected acaricides against five stored product mites under laboratory assay. Journal of Pest Science 84, 387-391. Go to original source...
    23. Volpato A, Lorenzetti W, Zortea T, Giombelli L, Baretta D, Santos R, Vaucher R, Raffin R, Souza M, Stefani L (2016): Melaleuca alternifolia essential oil against the lesser mealworm (Alphitobius diaperinus) and its possible effect on the soil fauna. Revista Brasileira de Ciencia Avicola 18, 41-46. Go to original source...
    24. Xuanji X, Zengjun G, Hui Z, Xia L, Jun L, Dandan L, Jun L (2016): Chemical composition, in vitro antioxidant activity and α-glucosidase inhibitory effects of the essential oil and methanolic extract of Elsholtzia densa Benth. Natural Product Research 30, 2707-2711. Go to original source... Go to PubMed...

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