Vet Med - Czech, 2020, 65(3):134-142 | DOI: 10.17221/152/2019-VETMED

Effect of single or combined supplementation of zinc and probiotics on muscle and bone characteristics and haematobiochemical profile in broilersOriginal Paper

M Shah1, H Zaneb2, S Masood2, AS Qureshi3, HA Ullah1, A Sikandar4, S Din2, I Ahmad1, MS Khan5, H Ur Rehman6, M Usman3
1 Department of Animal Health, The University of Agriculture, Peshawar, Pakistan
2 Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore, Pakistan
3 Department of Anatomy, University of Agriculture, Faisalabad, Pakistan
4 Department of Basic Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
5 Department of Bio-Sciences, Gomal University D. I. Khan, Dera Ismail Khan, Pakistan
6 Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

The study was conducted to elucidate the effect of a single or combined supplementation of zinc sulphate monohydrate (ZnSO4.H2O) and a probiotic (Protexin®) on the pectoral muscle, tibial bone and serum biochemistry in broilers. Day-old chicks (n = 192) were divided into: Control (basal diet), Zn30 (basal diet + ZnSO4.H2O 30 mg/kg feed), Zn60 (basal diet + ZnSO4.H2O 60 mg/kg feed), Pro (basal diet + Protexin® 0.1 g/kg feed), Com30 (basal diet + ZnSO4.H2O 30 mg/kg feed + Protexin® 0.1 g/kg feed) and Com60 (basal diet + ZnSO4.H2O 60 mg/kg feed + Protexin® 0.1 g/kg feed). The birds were slaughtered on the 42nd day and blood was collected to determine the cortisol, cholesterol and zinc concentrations. The pectoral muscle was selected for the assessment of the pH and water holding capacity (WHC) along with the histological sections. After defleshing, tibial bone measurements were also taken. Statistical analysis revealed a significant (P < 0.05) increase in the ultimate pH (pHu), WHC and muscle fascicle diameter in the Zn30, Zn60, Pro, Com30 and Com60 groups compared to the control group. Myofibre area showed a significant increase (P > 0.05) in the Com30 and Com60 groups against the control group. The medullary canal diameter of the tibia was smaller (P > 0.05) in the Zn60, Pro, Com30 and Com60 groups than in the control group. The tibiotarsal index was greater (P > 0.05) in the Zn60, Pro, Com30 and Com60 groups. The serum cholesterol was decreased (P > 0.05) in the Pro and Com30 groups when compared to the control group. It is concluded that zinc sulfate monohydrate and probiotic supplementation individually or in combination positively affected the histological characteristics of the muscle, tibial bone strength and haematobiochemical indicators.

Keywords: zinc sulphate monohydrate; Protexin®; meat; tibia; cholesterol

Received: October 29, 2019; Accepted: January 29, 2020; Published: March 31, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Shah M, Zaneb H, Masood S, Qureshi A, Ullah H, Sikandar A, et al.. Effect of single or combined supplementation of zinc and probiotics on muscle and bone characteristics and haematobiochemical profile in broilers. Vet Med - Czech. 2020;65(3):134-142. doi: 10.17221/152/2019-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aksu MI, Karaoglu M, Esenbuga N, Kaya M, Macit M, Ockerman H. Effect of a dietary probiotic on some quality characteristics of raw broiler drumsticks and breast meat. J Muscle Foods. 2005 Oct;16(4):306-17. Go to original source...
    2. Al-Daraji HJ, Amen MH. Effect of dietary zinc on certain blood traits of broiler breeder chickens. Int J Poult Sci. 2011 Oct;10(10):807-13. Go to original source...
    3. Al-Kassie GA, Al-Jumaa YM, Jameel YJ. Effect of probiotic (Aspergillus niger) and prebiotic (Taraxacum officinale) on blood picture and biochemical properties of broiler chicks. Int J Poult Sci. 2008 Dec;7(12):1182-4. Go to original source...
    4. Alam S, Masood S, Zaneb H, Rabbani I, Khan RU, Shah M, Ashraf S, Alhidary IA. Effect of Bacillus cereus and phytase on the expression of musculoskeletal strength and gut health in Japanese Quail (Coturnix japonica). Poult Sci J. 2019:0190057. Go to original source... Go to PubMed...
    5. Ali FH. "Probiotics feed supplement" to improve quality of broiler chicken carcasses. World J Dairy Food Sci. 2010 Jan;5(1):93-9.
    6. Bancroft JD, Floyd AD, Suvarna SK. Bancroft's theory and practice of histological techniques. 7th ed. New York, NY: Elsevier Health Sci; 2013. 637 p.
    7. Chand N, Zahirullah, Khan RU, Shah M, Naz S, Tinelli A. Zinc source modulates zootechnical characteristics, intestinal features, humoral response and paraoxonase (PON1) activity in broilers. Trop Anim Health Prod. 2019 Aug 17, 1-5. Go to original source... Go to PubMed...
    8. Garcia RG, de-Freitas L, Schwingel A, Farias R, Caldara FR, Gabriel F, Graciano J, Komiyama C, Almeida-Paz I. Incidence and physical properties of PSE chicken meat in a commercial processing plant. Braz J Poultry Sci. 2010 Dec;12(4):233-7. Go to original source...
    9. Genchev A, Mihaylova G, Ribarski S, Pavlov S, Kabakchiev S. Meat quality and composition in Japanese quails. Trakia J Sci. 2008 Jan;6(4):72-82.
    10. Hancock R, Viola R. The use of micro-organisms for Lascorbic acid production: current status and future perspectives. Appl Microbiol Biotechnol. 2001 Sep 1; 56(5-6):567-76. Go to original source... Go to PubMed...
    11. Joo ST, Kim GD, Hwang YH, Ryu YC. Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Sci. 2013 Dec 1;95(4):828-36. Go to original source... Go to PubMed...
    12. Khan RU, Naz S. Applications of probiotics in poultry production. Worlds Poult Sci J. 2013 Sep;69(3):621-32. Go to original source...
    13. Khan RU, Nikousefat Z, Javadani M, Tufarelli V, Laudadio V. Zinc-induced molting: production and physiology. Worlds Poult Sci J. 2011 Sep 1;67(3):497-506. Go to original source...
    14. Khan RU, Rahman ZU, Javed I, Muhammad F. Supplementation of dietary vitamins, protein and probiotics on semen traits and immunohistochemical study of pituitary hormones in zinc-induced molted broiler breeders. Acta Histochem. 2013 Sep 1;115(7):698-704. Go to original source... Go to PubMed...
    15. Khan RU, Zia-ur-Rahman, Javed I, Muhammad F. Serum antioxidants and trace minerals as influenced by vitamins, probiotics and proteins in broiler breeders. J Appl Anim Res. 2014 Jul 3;42(3):249-55. Go to original source...
    16. Kocabagli N. The effect of dietary phytase supplementation at different levels on tibial bone characteristics and strength in broilers. Turk J Vet Anim Sci. 2001 Sep 12;25(5):797-802.
    17. Liu Z, Lu L, Li S, Zhang L, Xi L, Zhang K, Luo X. Effects of supplemental zinc source and level on growth performance, carcass traits, and meat quality of broilers. Poult Sci. 2011 Aug 1;90(8):1782-90. Go to original source... Go to PubMed...
    18. Mansoub NH. Effect of probiotic bacteria utilization on serum cholesterol and triglycerides contents and performance of broiler chickens. Global Vet. 2010 Jan; 5(3):184-6.
    19. Mohanna C, Nys Y. Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens. Br Poult Sci. 1999 Mar 1;40(1):108-14. Go to original source... Go to PubMed...
    20. Mutus R, Kocabagli N, Alp N, Acar N, Eren M, Gezen S. The effect of dietary probiotic supplementation on tibial bone characteristics and strength in broilers. Poult Sci. 2006 Sep 1;85(9):1621-5. Go to original source... Go to PubMed...
    21. Naz S, Idris M, Khalique MA, Zia-ur-Rahman, Alhidary IA, Abdelrahman MM, Khan RU, Chand N, Farooq U, Ahmad S. The activity and use of zinc in poultry diet. Worlds Poult Sci J. 2016 Mar;72(1):159-67. Go to original source...
    22. National Research Council. Nutrient requirements of poultry. 9th ed. Washington D.C., National Academy Press, Washington D.C.; 1994.
    23. Owens C, Hirschler E, Martinez-Dawson R, Sams A. The characterization and incidence of pale, soft, exudative turkey meat in a commercial plant. Poult Sci. 2000 Apr 1;79(4):553-8. Go to original source... Go to PubMed...
    24. Qiao M, Fletcher D, Smith D, Northcutt J. The effect of broiler breast meat color on pH, moisture, water-holding capacity, and emulsification capacity. Poult Sci. 2001 May 1;80(5):676-80. Go to original source... Go to PubMed...
    25. Rasmussen A, Andersson M. New method for determination of drip loss in pork muscles In: Proceedings 42nd International Congress of Meat Science and Technology; 1-6 September, Lillehammer, Norway; 1996. p. 286-9.
    26. Rehfeldt C, Kuhn G. Consequences of birth weight for postnatal growth performance and carcass quality in pigs as related to myogenesis. J Anim Sci. 2006 Apr 1;84 Suppl:E113-23. Go to original source... Go to PubMed...
    27. Rehfeldt C, Stickland NC, Fiedler I, Wegner J. Environmental and genetic factors as sources of variation in skeletal muscle fibre number. BAM. 1999 Jan;9(5):235-53.
    28. Sahraei M, Janmmohamdi H, Taghizadeh A, Cheraghi S. Effect of different zinc sources on tibia bone morphology and ash content of broiler chickens. Adv Biol Res. 2012 Jan;6(3):128-32.
    29. Saleem MU. Relationship of gender and body condition score on histomorphometric and physical characteristics of selected skeletal muscle in Kundi buffalo [dissertation]. Lahore: UVAS; 2012.
    30. Salim H, Lee H, Jo C, Lee S, Lee BD. Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. Biol Trace Elem Res. 2012 Jun 1;147(1-3):120-9. Go to original source... Go to PubMed...
    31. Shah M, Zaneb H, Masood S, Khan RU, Ashraf S, Sikandar A, Rehman HFU, Rehman H. Effect of dietary supplementation of zinc and multi-microbe probiotic on growth traits and alteration of intestinal architecture in broiler. Probiotics Antimicrob Proteins. 2019a Sep 15;11(3):931-937. Go to original source... Go to PubMed...
    32. Shah M, Zaneb H, Masood S, Khan RU, Din S, Khan I, Tariq A, ur Rehman H. Ameliorative effect of zinc and multistrain probiotic on muscle and bone characteristics in broiler reared under cyclic heat stress. Pak J Zool. 2019b Jun 30;51(3):1041. Go to original source...
    33. Vahdatpour T, Ebrahimnezhad Y, Vahdatpour S. Effects of dietary functional additives on characteristics and minerals of tibia bone and blood parameters of Japanese quails (Coturnix coturnix japonica). Int J Plant, Anim Environ Sci. 2014 Apr-Jun;4(2):690-5.
    34. Weber P. The role of vitamins in the prevention of osteoporosis-a brief status report. Int J Vitam Nutr Res. 1999 May 1;69(3):194-7. Go to original source... Go to PubMed...
    35. Yang XJ, Sun XX, Li CY, Wu XH, Yao JH. Effects of copper, iron, zinc, and manganese supplementation in a corn and soybean meal diet on the growth performance, meat quality, and immune responses of broiler chickens. J Appl Poultry Res. 2011 Oct 1;20(3):263-71. Go to original source...
    36. Zhang A, Lee B, Lee S, Lee K, An G, Song K, Lee C. Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance, meat quality, and ileal mucosa development of broiler chicks. Poult Sci. 2005 Jul 1; 84(7):1015-21. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content