Vet Med - Czech, 2023, 68(5):200-207 | DOI: 10.17221/6/2023-VETMED

In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mitesOriginal Paper

F Liao1, T Bao2, G Tao3, Y Hu1, C Han3
1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, P.R. China
2 Guizhou Vocational College of Agriculture, Qingzhen, P.R. China
3 Qiandongnan Center for Animal Disease Control and Prevention, Kaili, Guizhou, P.R. China

In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites.

Keywords: acaricidal properties; LC-MS; sarcoptic mange; Urtica fissa

Received: January 13, 2023; Revised: April 29, 2023; Accepted: May 11, 2023; Prepublished online: May 24, 2023; Published: May 29, 2023  Show citation

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Liao F, Bao T, Tao G, Hu Y, Han C. In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites. Vet Med - Czech. 2023;68(5):200-207. doi: 10.17221/6/2023-VETMED.
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    References

    1. Alimi D, Hajri A, Jallouli S, Sebai H. In vitro acaricidal activity of essential oil and crude extracts of Laurus nobilis, (Lauraceae) grown in Tunisia, against arthropod ectoparasites of livestock and poultry: Hyalomma scupense and Dermanyssus gallinae. Vet Parasitol. 2021 Oct;298:109507. Go to original source... Go to PubMed...
    2. Alsamri H, Hasasna HE, Baby B, Alneyadi A, Dhaheri YA, Ayoub MA, Eid AH, Vijayan R, Iratni R. Carnosol is a novel inhibitor of p300 acetyltransferase in breast cancer. Front Oncol. 2021 May 13;11:664403. Go to original source... Go to PubMed...
    3. Benelli G, Canale A, Toniolo C, Higuchi A, Murugan K, Pavela R, Nicoletti M. Neem (Azadirachta indica): Towards the ideal insecticide? Nat Prod Res. 2017 Feb;31(4):369-86. Go to original source... Go to PubMed...
    4. De Melo GO, Muzitano MF, Legora-Machado A, Almeida TA, De Oliveira DB, Kaiser CR, Koatz VL, Costa SS. C-glycosylflavones from the aerial parts of Eleusine indica inhibit LPS-induced mouse lung inflammation. Planta Med. 2005 Apr;71(4):362-3. Go to original source... Go to PubMed...
    5. El-Sawy E, Abo-Salem H, Mandour A. 1H-indole-3-carboxaldehyde: Synthesis and reactions. Egypt J Chem. 2017;60(5):723-51. Go to original source...
    6. Gopinath H, Aishwarya M, Karthikeyan K. Tackling scabies: Novel agents for a neglected disease. Int J Dermatol. 2018 Nov;57(11):1293-8. Go to original source... Go to PubMed...
    7. Hajhashemi V, Klooshani V. Antinociceptive and anti-inflammatory effects of Urtica dioica leaf extract in animal models. Avicenna J Phytomed. 2013 Spring;3(2):193-200.
    8. Hao PY, Feng YL, Zhou YS, Song XM, Li HL, Ma Y, Ye CL, Yu XP. Schaftoside interacts with NlCDK1 protein: A mechanism of rice resistance to brown planthopper, Nilaparvata lugens. Front Plant Sci. 2018 May 29;9:710. Go to original source... Go to PubMed...
    9. Hu Y, Liao F, Hu Y, Luo B, He Y, Mo Q, Zuo Z, Ren Z, Deng J, Wei Y. Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits. BMC Vet Res. 2014 Dec 20;10(5):970. Go to original source...
    10. Khan A, Sohaib M, Ullah R, Hussain I, Niaz S, Malak N, de la Fuente J, Khan A, Aguilar-Marcelino L, Alanazi AD, Ben Said M. Structure-based in silico design and in vitro acaricidal activity assessment of Acacia nilotica and Psidium guajava extracts against Sarcoptes scabiei var. cuniculi. Parasitol Res. 2022 Oct;121(10):2901-15. Go to original source... Go to PubMed...
    11. Li Y, Wang YL, Li L, Liu L, Lu YX, Cheng XC, Zhang QL. Structural characterization of polysaccharides from the roots of Urtica fissa. J Asian Nat Prod Res. 2009 Nov;11(11):951-7. Go to original source... Go to PubMed...
    12. Liao F, Hu Y, Tan H, Wu L, Wang Y, Huang Y, Mo Q, Wei Y. Acaricidal activity of 9-oxo-10,11-dehydroageraphorone extracted from Eupatorium adenophorum in vitro. Exp Parasitol. 2014 May;140:8-11. Go to original source... Go to PubMed...
    13. Luo B, Liao F, Hu Y, Liu XI, He Y, Wu L, Tan H, Luo L, Zhou Y, Mo Q, Deng J, Wei Y. Acaricidal activity of extracts from Ligularia virgaurea against the Sarcoptes scabiei mite in vitro. Exp Ther Med. 2015 Jul;10(1):247-50. Go to original source... Go to PubMed...
    14. Lynar S, Currie BJ, Baird R. Scabies and mortality. Lancet Infect Dis. 2017 Dec;17(12):1234. Go to original source... Go to PubMed...
    15. Mao GN, He YJ, Yang WJ, Hu Y, Hou JL. [Effects of Urtica fissa extract on glycolipid metabolism in type 2 diabetic mice]. Prog Vet Med. 2020;41(1):61-5. Chinese.
    16. Montecino-Latorre D, Cypher BL, Rudd JL, Clifford DL, Mazet JAK, Foley JE. Assessing the role of dens in the spread, establishment and persistence of sarcoptic mange in an endangered canid. Epidemics. 2019 Jun;27:28-40. Go to original source... Go to PubMed...
    17. Nong X, Ren YJ, Wang JH, Xie Y, Fang CL, Yang DY, Liu TF, Zhang RH, Chen L, Gu XB, Peng XR, Wang SX, Lai SJ, Yang GY. Clinical efficacy of botanical extracts from Eupatorium adenophorum against the Sarcoptes scabiei (Sarcoptidae: Sarcoptes) in rabbits. Vet Parasitol. 2013 Jul 1;195(1-2):157-64. Go to original source... Go to PubMed...
    18. Pasipanodya CN, Tekedza TT, Chatiza FP, Gororo E. Efficacy of neem (Azadirachta indica) aqueous fruit extracts against Sarcoptes scabiei var. suis in grower pigs. Trop Anim Health Prod. 2021 Jan 22;53(1):135. Go to original source... Go to PubMed...
    19. Singh SK, Jaiswal AK, Kumari S, Gaur RS, Sharma P, Khushboo R, Jaiswal M. Therapeutic effects of oral fluralaner in pet rabbits with severe sarcoptic mange (Sarcoptes scabiei). Vet Parasitol. 2022 Apr;304:109693. Go to original source... Go to PubMed...
    20. Upton R. Stinging nettles leaf (Urtica dioica L.): Extraordinary vegetable medicine. J Herb Med. 2013;3(1):9-38. Go to original source...
    21. Wang M, Feng X, Zhang C, Cheng J, Liu E, Sun L, Li X. The chemical constituents in the decoction of Urtica fissa rhizomes. J Chin Pharm Sci. 2016;25(10):764-70. Go to original source...
    22. Wang MY, Yang Y, Zhou XY, Wang SS, Duan SX, Li XB. The chemical constituents from Urtica fissa leaves. J Asian Nat Prod Res. 2018 Aug;20(8):709-18. Go to original source... Go to PubMed...
    23. Wang M, Wu L, Zhang H, Ren DZC, Liu T, Li X. The antioxidant compounds from Urtica fissa flowers. Chem Nat Compd. 2019;55(5):839-42. Go to original source...
    24. Wang CX, Wang ZQ, Li YX, Bai XS, Chen L, Huang XZ, Li YH. [Studies on chemical constituents of Urtica fissa leaves]. Yunnan Minzu Univ. 2022 [cited 2023 May 2]. Available from: https://kns.cnki.net/kcms/detail/53.1192.N.20220615.1326.002.html. Chinese.
    25. Want EJ, Masson P, Michopoulos F, Wilson ID, Theodoridis G, Plumb RS, Shockcor J, Loftus N, Holmes E, Nicholson JK. Global metabolic profiling of animal and human tissues via UPLC-MS. Nat Protoc. 2013 Jan;8(1):17-32. Go to original source... Go to PubMed...
    26. Yang L, Sui Y, Zhong L, Ma T, Ma Z, Liu X. Carnosol inhibits the growth and biofilm of Candida albicans. J Mycol Med. 2022 May;32(2):101234. Go to original source... Go to PubMed...
    27. Yi Y, Zhang M, Xue H, Yu R, Bao YO, Kuang Y, Chai Y, Ma W, Wang J, Shi X, Li W, Hong W, Li J, Muturi E, Wei H, Wlodarz J, Roszak S, Qiao X, Yang H, Ye M. Schaftoside inhibits 3CLpro and PLpro of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19. Acta Pharm Sin B. 2022 Nov;12(11):4154-64. Go to original source... Go to PubMed...
    28. Zelena E, Dunn WB, Broadhurst D, Francis-McIntyre S, Carroll KM, Begley P, O'Hagan S, Knowles JD, Halsall A; HUSERMET Consortium; Wilson ID, Kell DB. Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum. Anal Chem. 2009 Feb 15;81(4):1357-64. Go to original source... Go to PubMed...
    29. Zhang H, Chen Y, Li XB, Deng WX, Wang MY. [Active constituents of Urtica fissa in inhibition of benign prostatic hyperplasia]. Zhongguo Zhong Yao Za Zhi. 2022 Jan;47(2):419-27. Chinese.
    30. Zhou K, Wu J, Chen J, Zhou Y, Chen X, Wu Q, Xu Y, Tu W, Lou X, Yang G, Jiang S. Schaftoside ameliorates oxygen glucose deprivation-induced inflammation associated with the TLR4/Myd88/Drp1-related mitochondrial fission in BV2 microglia cells. J Pharmacol Sci. 2019a Jan;139(1):15-22. Erratum in: J Pharmacol Sci. 2021 Jul;146(3):182. Go to original source... Go to PubMed...
    31. Zhou Y, Liao F, Weng J, Mo Q, Xu R, Zhang Y, Ren Z, Zhong Z, Zuo Z, Peng G, Deng J, Tang C, Hu Y. Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitro. Vet Med-Czech. 2019b Apr;64(4):178-83. Go to original source...

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