Vet Med - Czech, 2025, 70(3):101-109 | DOI: 10.17221/94/2024-VETMED
Effectiveness of glycerin-oxalic acid strips and essential oils in controlling Varroa destructor in honeybeeOriginal Paper
- 1 Agricultural Machinery Department, Agrarian and Industrial Faculty, University of Ruse, Ruse, Bulgaria
- 2 Institute of Animal Sciences, Kostinbrod, Bulgaria
The decline in the bee colony strength due to high levels of Varroa destructor infestations necessitates the development of new control methods. This study evaluates the effectiveness of glycerin-oxalic acid strips and essential oils in managing Varroa destructor. The experiment was conducted in 2022 at the experimental apiary in Debelec, part of the Institute of Animal Husbandry in Kostinbrod. Six experimental groups, each treated with a different preparation, were compared to a control group of untreated colonies. Treatments included Beevital Hiveclean (20 ml per colony), Varro Red (2 ml per frame), ammonium nitrate strips (1.3 mm), glycerin-oxalic acid strips (1.3 mm), and glycerin-oxalic acid strips of paper-cellulose (2.3 mm) or cellulose-cotton (2.3 mm). The control group remained untreated. The glycerin-oxalic acid strips made of cellulose and cotton (2.3 mm) showed the highest efficacy, reducing the mite infestation by 17.79% compared to the control. The glycerin-oxalic acid strips of paper-cellulose (2.3 mm) were also effective, achieving a 17.05% reduction in mite levels. These results provide valuable insights for beekeepers seeking alternative and sustainable methods for controlling Varroa destructor infestations.
Keywords: alternative treatment methods; apiculture health; mite infestation control; parasitic resistance
Received: October 14, 2024; Revised: January 30, 2025; Accepted: February 12, 2025; Prepublished online: March 24, 2025; Published: March 26, 2025 Show citation
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References
- Alsaadi M, Keshlaf MM, Mirwan HB. Some essential oils as potential control agents for varroa mite (Varroa destructor) in infected honey bees (Apis mellifera). Open Vet J. 2024 Feb;14(2):692-8.
Go to original source...
Go to PubMed...
- Atanasov AZ, Georgiev SG, Vulkov LG. Parameters reconstruction in modeling of honeybee colonies infested with Varroa destructor. AIP Conf Proc. 2022;2522:110005.
Go to original source...
- Bahreini R, Nasr M, Docherty C, Herdt de O, Muirhead S, Feindel D. Evaluation of potential miticide toxicity to Varroa destructor and honey bees, Apis mellifera, under laboratory conditions. Sci Rep. 2020 Dec 9;10(1):21529.
Go to original source...
Go to PubMed...
- Bartlett LJ, Baker C, Bruckner S, Delaplane KS, Hackmeyer EJ, Phankaew C, Williams GR, Berry JA. No evidence to support the use of glycerol-oxalic acid mixtures delivered via paper towel for controlling Varroa destructor (Mesostigmata: Varroidae) mites in the Southeast United States. J Insect Sci. 2023 Nov;23(6):18:1-5.
Go to original source...
Go to PubMed...
- Bava R, Castagna F, Lupia C, Ruga S, Musella V, Conforti F, Marrelli M, Argentieri MP, Britti D, Statti G. Chemical profile of essential oils of selected Lamiaceae plants and in vitro activity for varroosis control in honeybees (Apis mellifera). Vet Sci. 2023;10(701):1-16.
Go to original source...
Go to PubMed...
- Begna T, Ulziibayar D, Bisrat D, Jung C. Acaricidal toxicity of four essential oils, their predominant constituents, their mixtures against Varroa mite, and their selectivity to honey bees (Apis cerana and A. mellifera). Insects. 2023;14(735):1-16.
Go to original source...
Go to PubMed...
- Borsuk G, Olszewski K, Paleolog J, Strachecka A, Gryzinska M. The effect of different varroacides on the acidity of winter stores and honey stores. Ann UMCS Zootech. 2012;30:11-6.
Go to original source...
- Gaidar V. [Determining the entanglement of bee families - A pathway to their conservation]. Ukr Pasichnik. 2011;(12). Russian.
- Haber AI, Nathalie AS, vanEngelsdorp D. Use of chemical and nonchemical methods for the control of Varroa destructor (Acari: Varroidae) and associated winter colony losses in U.S. beekeeping operations. J Econ Entomol. 2019 Aug;112(4):1509-25.
Go to original source...
Go to PubMed...
- Hazam S, Touati S, Touati L. Promising Algerian essential oils as natural acaricides against the honey bee mite Varroa destructor (Acari: Varroidae). Exp Appl Acarol. 2024;92:87-107.
Go to original source...
Go to PubMed...
- Hernandez RM, Pascual MH, Perez J, del Nozal Nalda MJ, Meana A. Short-term negative effect of oxalic acid in Apis mellifera Iberiensis. Span J Agric Res. 2007;5:474-80.
Go to original source...
- Higes M, Meana A, Suarez M, Llorente J. Negative long-term effects on bee colonies treated with oxalic acid against Varroa jacobsoni Oud. Apidologie. 1999;30:289-92.
Go to original source...
- Hristov P, Ivanov H. Testing the healing effect of "Argentine tapes" in control and treatment of bee's varroasis. Zhivotnovadni Nauki. 2021;58(1):39-48.
- Hristov P, Shumkova R, Palova N, Neov B. Honey bee colony losses: Why are honey bees disappearing. Sociobiology. 2021;68(1).
Go to original source...
- Hristov P. Test of the acaricidal effect of the preparations "Eco plus", "Devar", "Bisanar" and "Beevital Hiveclean". Zhivotnovadni Nauki. 2021;58(5).
- Hybl M, Bohata A, Radsetoulalova I, Kopecky M, Hostickova I, Vanickova A, Mraz P. Evaluating the efficacy of 30 different essential oils against Varroa destructor and honey bee workers (Apis mellifera). Insects. 2021;12:1045.
Go to original source...
Go to PubMed...
- Jack CJ, van Santen E, Ellis JD. Determining the dose of oxalic acid applied via vaporization needed for the control of the honey bee (Apis mellifera) pest Varroa destructor. J Apic Res. 2021;60(3):414-20.
Go to original source...
- Kovacic M, Uzunov A, Tlak Gajger I, Pietropaoli M, Soroker V, Adjlane N, Benko V, Charistos L, Dall'Olio R, Formato G. Honey vs. mite - A trade-off strategy by applying summer brood interruption for Varroa destructor control in the Mediterranean region. Insects. 2023;14:751.
Go to original source...
Go to PubMed...
- Lakin GF. Biometrics. Moscow: Vysshaya Shkola; 1980. p. 104-7. Russian.
- Lambert HB, Adamczyk KJ, Marshall K, Cox R. Monitoring for resistance to organophosphorus and pyrethroid insecticides in Varroa mite populations. J Econ Entomol. 2010 Oct;103(5):1797-802.
Go to original source...
Go to PubMed...
- Lazarov S, Dineva G. An innovative method for determining the area of the capped brood in bee colonies. J Hyg Eng Des. 2022;(39):236-41.
- Le Conte Y, Ellis M, Ritter W. Varroa mites and honey bee health: Can varroa explain part of the colony losses. Apidologie. 2010;41:353-63.
Go to original source...
- Messan K, Messan MR, Chen J, Deg-Hoffman G, Kang Y. Population dynamics of Varroa mite and honeybee: Effects of parasitism with age structure and seasonality. arXiv. 2020;2003.12089v2[q-bio.PE].
Go to original source...
- Narciso L, Topini M, Ferraiuolo S, Ianiro G, Marianelli C. Effects of natural treatments on the varroa mite infestation levels and overall health of honey bee (Apis mellifera) colonies. PLoS One. 2024;19(5):eXXX.
Go to original source...
Go to PubMed...
- Palmer K. Honey bee and Varroa destructor population dynamics. Dakota: Dept Math Sci, Univ South Dakota; 2019.
- Parvanov RP. [Biology and diseases of bees]. Stara Zagora: Alfa Vision; 2016. Bulgarian.
- Prouty C, Abou-Shaara HF, Stanford B, Ellis JD, Jack C. Oxalic acid application method and treatment intervals for reduction of Varroa destructor (Mesostigmata: Varroidae) populations in Apis mellifera (Hymenoptera: Apidae) colonies. J Insect Sci. 2023 Nov;23(6):13.
Go to original source...
Go to PubMed...
- Ramsey SD, Ochoa R, Bauchan G, Gulbronson C, Mowery JD, Cohen A, Lim D, Joklik J, Cicero JM, Ellis JD. Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph. Proc Natl Acad Sci U S A. 2019;116:1792-801.
Go to original source...
Go to PubMed...
- Ratti V, Kevan PG, Eberl HJ. A mathematical model for population dynamics in honeybee colonies infested with Varroa destructor and acute bee paralysis virus. Can Appl Math Q. 2013;21(1):63-93.
- Rinkevich FD. Detection of amitraz resistance and reduced treatment efficacy in the varroa mite, Varroa destructor, within commercial beekeeping operations. PLoS One. 2020;15:eXXX.
Go to original source...
Go to PubMed...
- Roth MA, Wilson JM, Gross AD. Assessing Varroa destructor acaricide resistance in Apis mellifera colonies of Virginia. Apidologie. 2021;52:1278-90.
Go to original source...
- Stara J, Pekar S, Nesvorna M, Erban T, Vinsova H, Kopecky J, Doskocil I, Kamler M, Hubert J. Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR-RFLP of kdr mutation in sodium channel gene. Exp Appl Acarol. 2019 Feb;77(2):161-71.
Go to original source...
Go to PubMed...
- Warner S, Pokhrel LR, Akula SM, Ubah CS, Richards SL, Jensen H, Kearney GD. A scoping review on the effects of Varroa mite (Varroa destructor) on global honey bee decline. Sci Total Environ. 2024;906:167492.
Go to original source...
Go to PubMed...
- Yang X, Cox-Foster DL. Impact of an ectoparasite on the immunity and pathology of an invertebrate: Evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci U S A. 2005;102:7470-5.
Go to original source...
Go to PubMed...
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