Vet Med - Czech, 2025, 70(5):166-176 | DOI: 10.17221/65/2024-VETMED

Antibacterial activity and compositional analysis of Acer truncatum bunge leaf extract against Staphylococcus aureusOriginal Paper

F Liao, J He
Guizhou Vocational College of Agriculture, Qingzhen, P.R. China

Staphylococcus aureus is a zoonotic pathogen that can cause various diseases in both humans and animals. This experiment evaluates the antibacterial activity of extracts from Acer truncatum leaves against S. aureus, including the minimum inhibitory concentration (MIC), growth curve, and cell membrane integrity assessment, alongside the identification of the extract components by LC-MS. Results demonstrated that the MIC of the n-butanol extract from the ethanol extract of Acer truncatum leaves against S. aureus was 3.125 mg/ml, with a minimum bactericidal concentration (MBC) of 6.25 mg/ml. Over 16 h, the extracts at concentrations of 0.25 MIC, 0.5 MIC, and 1 MIC effectively inhibited S. aureus growth. The fluorescence staining revealed that the extracts at different concentrations compromised the structural integrity of the cell membrane. The LC-MS analysis identified the principal constituents of the extract as betaine (27.189%), 3,4-dihydroxymandelic acid (16.112%), quercitrin (14.768%), chlorogenic acid (8.778%) and neochlorogenic acid (4.452%). The study indicated that Acer truncatum leaf extract has good potential for application in natural antibacterial drugs.

Keywords: antimicrobial agents; cell membrane integrity; LC-MS analysis; phenolic compounds

Received: July 30, 2024; Revised: April 3, 2025; Accepted: April 9, 2025; Prepublished online: May 28, 2025; Published: May 29, 2025  Show citation

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Liao F, He J. Antibacterial activity and compositional analysis of Acer truncatum bunge leaf extract against Staphylococcus aureus. Vet Med - Czech. 2025;70(5):166-176. doi: 10.17221/65/2024-VETMED.
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    References

    1. Berti AD, Baines SL, Howden BP, Sakoulas G, Nizet V, Proctor RA, Rose WE. Heterogeneity of genetic pathways toward daptomycin nonsusceptibility in Staphylococcus aureus determined by adjunctive antibiotics. Antimicrob Agents Chemother. 2015 May;59(5):2799-806. Go to original source... Go to PubMed...
    2. Bismelah NA, Ahmad R, Mohamed Kassim ZH, Ismail NH, Rasol NE. The antibacterial effect of Plectranthus scutellarioides (L.) R.Br. leaves extract against bacteria associated with peri-implantitis. J Tradit Complement Med. 2022 Aug 3;12(6):556-66. Go to original source... Go to PubMed...
    3. Bittner Fialova S, Rendekova K, Mucaji P, Nagy M, Slobodnikova L. Antibacterial activity of medicinal plants and their constituents in the context of skin and wound infections, considering european legislation and folk medicine - A review. Int J Mol Sci. 2021 Oct 4;22(19):10746. Go to original source... Go to PubMed...
    4. Chen J, Li G, Sun C, Peng F, Yu L, Chen Y, Tan Y, Cao X, Tang Y, Xie X, Peng C. Chemistry, pharmacokinetics, pharmacological activities, and toxicity of Quercitrin. Phytother Res. 2022 Apr;36(4):1545-75. Go to original source... Go to PubMed...
    5. Costa SS, Ribeiro R, Serrano M, Oliveira K, Ferreira C, Leal M, Pomba C, Couto I. Staphylococcus aureus causing skin and soft tissue infections in companion animals: Antimicrobial resistance profiles and clonal lineages. Antibiotics (Basel). 2022 Apr 29;11(5):599. Go to original source... Go to PubMed...
    6. Deepak SJ, Kannan P, Savariraj WR, Ayyasamy E, Tuticorin Maragatham Alagesan SK, Ravindran NB, Sundaram S, Mohanadasse NQ, Kang Q, Cull CA, Amachawadi RG. Characterization of Staphylococcus aureus isolated from milk samples for their virulence, biofilm, and antimicrobial resistance. Sci Rep. 2024 Oct 27;14(1):25635. Go to original source... Go to PubMed...
    7. Deng M, Wang Y, Chen G, Liu J, Wang Z, Xu H. Poly-l-lysine-functionalized magnetic beads combined with polymerase chain reaction for the detection of Staphylococcus aureus and Escherichia coli O157:H7 in milk. J Dairy Sci. 2021 Dec;104(12):12342-52. Go to original source... Go to PubMed...
    8. Ekiert HM, Szopa A. Biological activities of natural products. Molecules. 2020 Dec 7;25:5769. Go to original source... Go to PubMed...
    9. Fan H, Sun L, Yang L, Zhou J, Yin P, Li K, Xue Q, Li X, Liu Y. Assessment of the bioactive phenolic composition of Acer truncatum seed coat as a byproduct of seed oil. Ind Crops Prod. 2018 Aug;118:11-9. Go to original source...
    10. Fan Y, Lin F, Zhang R, Wang M, Gu R, Long C. Acer truncatum Bunge: A comprehensive review on ethnobotany, phytochemistry and pharmacology. J Ethnopharmacol. 2022 Jan 10;282:114572. Go to original source... Go to PubMed...
    11. Fu T, Houel E, Amusant N, Touboul D, Genta-Jouve G, Della-Negra S, Fisher GL, Brunelle A, Duplais C. Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates. Sci Rep. 2019 Feb 13;9(1):1928. Go to original source... Go to PubMed...
    12. Gao F, Wang T, Xiao J, Huang G. Antibacterial activity study of 1,2,4-triazole derivatives. Eur J Med Chem. 2019 Jul 1;173:274-81. Go to original source... Go to PubMed...
    13. Gao XH, Zhang SD, Wang LT, Yu L, Zhao XL, Ni HY, Wang YQ, Wang JD, Shan CH, Fu YJ. Anti-inflammatory effects of neochlorogenic acid extract from mulberry leaf (Morus alba L.) against LPS-stimulated inflammatory response through mediating the AMPK/Nrf2 signaling pathway in A549 cells. Molecules. 2020 Mar 18;25(6):1385. Go to original source... Go to PubMed...
    14. Garg S, Roy A. A current perspective of plants as an antibacterial agent: A review. Curr Pharm Biotechnol. 2020;21(15):1588-602. Go to original source... Go to PubMed...
    15. Giao MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW. Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila. Microb Ecol. 2009 Jul;58(1):56-62. Go to original source... Go to PubMed...
    16. He X, Li DZ, Tian B. Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid. Plant Divers. 2020 Oct 31;43(1):86-92. Go to original source... Go to PubMed...
    17. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002 Oct;13(10):572-84. Go to original source... Go to PubMed...
    18. Ji Q, Zhang M, Wang Y, Chen Y, Wang L, Lu X, Bai L, Wang M, Bao L, Hao H, Wang Z. Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells. Microb Pathog. 2022 Oct;171:105726. Go to original source... Go to PubMed...
    19. Kim M, Choi SY, Lee P, Hur J. Neochlorogenic acid inhibits lipopolysaccharide-induced activation and pro-inflammatory responses in BV2 microglial cells. Neurochem Res. 2015 Sep;40(9):1792-8. Go to original source... Go to PubMed...
    20. Li Y, Kong F, Wu S, Song W, Shao Y, Kang M, Chen T, Peng L, Shu Q. Integrated analysis of metabolome, transcriptome, and bioclimatic factors of Acer truncatum seeds reveals key candidate genes related to unsaturated fatty acid biosynthesis, and potentially optimal production area. BMC Plant Biol. 2024 Apr 16;24(1):284. Go to original source... Go to PubMed...
    21. Li Y, Yu X, Deng L, Zhou S, Wang Y, Zheng X, Chu Q. Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food Funct. 2021 Sep 17;12(22):11387-98. Go to original source... Go to PubMed...
    22. Liao F, He J, Li R, Hu Y. Endophytic fungus UJ3-2 from Urtica fissa: Antibacterial activity and mechanism of action against Staphylococcus aureus. Molecules. 2024 Oct 13;29(20):4850. Go to original source... Go to PubMed...
    23. Liu B, Chen F, Bi C, Wang L, Zhong X, Cai H, Deng X, Niu X, Wang D. Quercitrin, an inhibitor of Sortase A, interferes with the adhesion of Staphylococcal aureus. Molecules. 2015 Apr 13;20(4):6533-43. Go to original source... Go to PubMed...
    24. Miao M, Xiang L. Pharmacological action and potential targets of chlorogenic acid. Adv Pharmacol. 2020 Jan;87:71-88. Go to original source... Go to PubMed...
    25. Nunes R, Pasko P, Tyszka-Czochara M, Szewczyk A, Szlosarczyk M, Carvalho IS. Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs. Pharm Biol. 2017 Dec;55(1):114-23. Go to original source... Go to PubMed...
    26. Perrine-Walker F, Le K. Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy. Protoplasma. 2021 Mar;258(2):279-87. Go to original source... Go to PubMed...
    27. Peton V, Le Loir Y. Staphylococcus aureus in veterinary medicine. Infect Genet Evol. 2014 Jan;21:602-15. Go to original source... Go to PubMed...
    28. Rodriguez-Perez C, Quirantes-Pine R, Uberos J, Jimenez-Sanchez C, Pena A, Segura-Carretero A. Antibacterial activity of isolated phenolic compounds from cranberry (Vaccinium macrocarpon) against Escherichia coli. Food Funct. 2016 Mar;7(3):1564-73. Go to original source... Go to PubMed...
    29. Rosenberg M, Azevedo NF, Ivask A. Propidium iodide staining underestimates viability of adherent bacterial cells. Sci Rep. 2019 Apr 24;9(1):6483. Go to original source... Go to PubMed...
    30. Lan SB. Research overview and development prospect of acer linn plants. Fore by-Prod Spec Chi. 2019;162(5):84-9.
    31. Silva S, Costa EM, Machado M, Morais R, Calhau C, Pintado M. Antiadhesive and antibiofilm effect of malvidin-3-glucoside and malvidin-3-glucoside/neochlorogenic acid mixtures upon staphylococcus. Metabolites. 2022 Nov 3;12(11):1062. Go to original source... Go to PubMed...
    32. Song J, GuangXin L, HanShen W, Meng L. [Antimicrobial activity and mechanism of sanguinarine against Streptococcus aga-lactiae]. Chinese J Vet Sci. 2021;41(6):1067-73. Chinese.
    33. Stiefel P, Schmidt-Emrich S, Maniura-Weber K, Ren Q. Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide. BMC Microbiol. 2015 Feb 18;15:36. Go to original source... Go to PubMed...
    34. Tan S, Cho K, Nodwell JR. A defect in cell wall recycling confers antibiotic resistance and sensitivity in Staphylococcus aureus. J Biol Chem. 2022 Oct;298(10):102473. Go to original source... Go to PubMed...
    35. Tasneem U, Mehmood K, Majid M, Ullah SR, Andleeb S. Methicillin resistant Staphylococcus aureus: A brief review of virulence and resistance. J Pak Med Assoc. 2022 Mar;72(3):509-15. Go to original source... Go to PubMed...
    36. Troscianczyk A, Nowakiewicz A, Kasela M, Malm A, Tracz AM, Hahaj-Siembida A, Osinska M, Gula S, Jankowiak I. Multi-host pathogen Staphylococcus aureus - Epidemiology, drug resistance and occurrence in humans and animals in Poland. Antibiotics (Basel). 2023 Jun 30;12(7):1137. Go to original source... Go to PubMed...
    37. Wang Q, Zhang F, Wang Z, Chen H, Wang X, Zhang Y, Li S, Wang H. Evaluation of the Etest and disk diffusion method for detection of the activity of ceftazidime-avibactam against Enterobacterales and Pseudomonas aeruginosa in China. BMC Microbiol. 2020 Jun 29;20(1):187. Go to original source... Go to PubMed...
    38. Wang XH, Guo R, Nie XB, Chen HM, Zhi WW, Tian ML, Liu F. [Antibacterial activity of Citri Sarcodactylis Fructus and its antibacterial mechanism against Staphylococcus aureus]. Chin J Antibiot. 2021;46:437-41. Chinese.
    39. Want EJ, Masson P, Michopoulos F, Wilson ID, Theodoridis G, Plumb RS, Shockcor J, Loftus N, Holmes E, Nicholson JK. Global metabolic profiling of animal and human tissues via UPLC-MS. Nat Protoc. 2013 Jan;8(1):17-32. Go to original source... Go to PubMed...
    40. Xu P, Xu XB, Khan A, Fotina T, Wang SH. Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract. Pol J Vet Sci. 2021 Jun;24(2):243-51. Go to original source... Go to PubMed...
    41. Yang X, Lan W, Xie J. Antimicrobial and anti-biofilm activities of chlorogenic acid grafted chitosan against Staphylococcus aureus. Microb Pathog. 2022a Dec;173(Pt A):105748. Go to original source... Go to PubMed...
    42. Yang YL, Adel Al-Mahdy D, Wu ML, Zheng XT, Piao XH, Chen AL, Wang SM, Yang Q, Ge YW. LC-MS-based identification and antioxidant evaluation of small molecules from the cinnamon oil extraction waste. Food Chem. 2022b Jan 1;366:130576. Go to original source... Go to PubMed...
    43. Zaatout N, Ayachi A, Kecha M. Staphylococcus aureus persistence properties associated with bovine mastitis and alternative therapeutic modalities. J Appl Microbiol. 2020 Nov;129(5):1102-19. Go to original source... Go to PubMed...
    44. Zelena E, Dunn WB, Broadhurst D, Francis-McIntyre S, Carroll KM, Begley P, O'Hagan S, Knowles JD, Halsall A; HUSERMET Consortium; Wilson ID, Kell DB. Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum. Anal Chem. 2009 Feb 15;81(4):1357-64. Go to original source... Go to PubMed...
    45. Zhao G, He F, Wu C, Li P, Li N, Deng J, Zhu G, Ren W, Peng Y. Betaine in inflammation: Mechanistic aspects and applications. Front Immunol. 2018 May 24;9:1070. Go to original source... Go to PubMed...
    46. Zhou H, Du W, Ouyang D, Li Y, Gong Y, Yao Z, Zhong M, Zhong X, Ye X. Simple and accurate genomic classification model for distinguishing between human and pig Staphylococcus aureus. Commun Biol. 2024 Sep 18;7(1):1171. Go to original source... Go to PubMed...

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