Vet Med - Czech, 2026, 71(4):139-146 | DOI: 10.17221/104/2025-VETMED

Oral efficacy of controlled-release doxycycline against Ichthyophthirius multifiliis infestation in salmonidsOriginal Paper

Z Mikulkova1, K Matejickova2, J Motlova2, P Ginterova1, M Jerabek1, L Pojezdal ORCID...2
1 Tekro, spol. s r.o., Prague, Czech Republic
2 Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic

Ichthyophthiriosis, caused by Ichthyophthirius multifiliis, is a major threat in salmonid aquaculture. This study evaluated the efficacy of orally administered standard doxycycline and controlled-release doxycycline formulations in experimentally infected brook trout (Salvelinus fontinalis). Fish received medicated feed for 10 days starting seven days post-infection. Controlled-release doxycycline achieved the highest survival rate (84.2%), followed by standard doxycycline (73.7%), while no control fish survived after day 25 post-infection. Both drug formulations eliminated trophonts from the skin and gills by 20 days post-treatment. Despite a 2.5-fold lower dietary dose, the controlled-release doxycycline achieved a tissue concentration equivalent to ~32% of that of standard doxycycline, with similar retention rates. Levels of doxycycline residues in water declined over time for both groups, indicating limited environmental release. These results demonstrate the effective mitigation of ichthyophthiriosis with orally administered doxycycline and the enhancement of antiparasitic efficacy and reduction of drug load in tissues and the environment via controlled-release technology, supporting its potential as a sustainable medicated feed strategy in salmonid aquaculture.

Keywords: brook trout; controlled release drug; medicated diet; rainbow trout

Received: December 4, 2025; Revised: January 23, 2026; Accepted: January 27, 2026; Prepublished online: April 27, 2026; Published: April 30, 2026  Show citation

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Mikulkova Z, Matejickova K, Motlova J, Ginterova P, Jerabek M, Pojezdal L. Oral efficacy of controlled-release doxycycline against Ichthyophthirius multifiliis infestation in salmonids. Veterinární medicína. 2026;71(4):139-146. doi: 10.17221/104/2025-VETMED.
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