Veterinární medicína, 2001 (vol. 46), issue 11-12

Serosurvey of sheep and goats to Chlamydia psittaci in Slovakia during the years 1996-2000Original Paper

M. Trávniček, D. Kováčová, P. Zubrický, L. Čisláková

Vet Med - Czech, 2001, 46(11):281-285 | DOI: 10.17221/7895-VETMED  

In the Slovak Republic during the years 1996-2000, total 30 278 blood samples from sheep and 4 756 from goats were examined serologically by the method of a complement fixation. The IgG antibodies against Chlamydia psittaci in sheep and goats according to individual years were as follows: 1996 - 6.37% sheep/3.94% goats, 1997 - 9.25%/10.02%, 1998 - 9.57%/2.96%, 1999 - 7.91%/3.69%, 2000 - 10.99%/6.08%, respectively. In most clinically healthy animals, the low levels of IgG antibodies in dilution of 1 : 16 to 1 : 32 were detected. In sheep during the years of 1996, 1998, 1999 and 2000 the high levels of IgG antibodies 1 : 256 to 1 : 1 024 were...

Effects of vitamin E and vitamin A supplementation on performance, thyroid status and serum concentrations of some metabolites and minerals in broilers reared under heat stress (32 degrees C)Original Paper

N. Sahin, K. Sahin, O. Küçük

Vet Med - Czech, 2001, 46(11):286-292 | DOI: 10.17221/7894-VETMED  

An experiment on Cobb-500 male broilers was conducted to evaluate the effects of vitamin E (alfa-tocopherol-acetate), vitamin A (retinol), and their combination on broiler performance and serum concentrations of triiodothyronine (T3), thyroxine (T4), adrenocorticotropine hormone (ACTH) and some metabolite and mineral concentrations in broilers reared under heat stress (32°C). One day-old 120 broilers were randomly assigned to 4 treatment groups, 3 replications of 10 birds each. The birds were fed either a control diet or a control diet supplemented with either vitamin A (15 000 IU retinol/kg diet), vitamin E (250 mg alfa-tocopherol-acetate/kg diet),...

Impaction of right dorsal colon in the horse: report of 32 casesOriginal Paper

J. Mezerová, R. Kabeš, Z. Žert, P. Jahn, J. Hanák

Vet Med - Czech, 2001, 46(11):293-300 | DOI: 10.17221/7892-VETMED  

Acute as well as chronic and recurrent colic episodes were caused by right dorsal colon (RDC) impaction in 7.98% of all colic patients. Significantly higher statistical evidence was figured out in hucul horses, mares and 7-year-old animals. Diagnosis of RDC impaction was established on the basis of rectal palpation (68.75%) or laparotomy (31.25%). Yield of rectal palpation was limited by a difficult accessability of RDC, small body frame of the animal, severe abdominal pain and by further physiological or pathological changes in abdominal cavity. Simple RDC impaction was diagnosed in 16 (50%) of 32 cases, in 9 horses the impaction associated with the...

Combined effects of repeated low doses of aflatoxin B-1 and T-2 toxin on the Chinese hamsterOriginal Paper

R. Rajmon, M. Sedmíková, F. Jílek, M. Koubková, H. Härtlová, I. Bárta, P. Šmerák

Vet Med - Czech, 2001, 46(11):301-307 | DOI: 10.17221/7893-VETMED  

The aim of this work was to find out how repeated low doses of aflatoxin B1 (AFB1) and T-2 toxin would influence the Chinese hamster and if the amplifying of these effects would occur with the application of both toxins together. The animals were treated with 10 ml/kg of 7% dimethylsulfoxid (DMSO) in the control group (C), 1.0 mg/kg of AFB1 in group A, 1.0 mg/kg of T-2 toxin in group T2, and 1.0 mg/kg of AFB1 + 1.0 mg/kg of T-2 toxin in group T2/A. All mycotoxins were dissolved in 10 ml/kg of 7% DMSO. These doses were administered intragastrically twice a week for a period of three weeks. General health condition, histological picture of some internal...

Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methodsReview

L. Dvorská, M. Bartoš, G. Martin, W. Erler, I. Pavlík

Vet Med - Czech, 2001, 46(11):309-328 | DOI: 10.17221/7890-VETMED  

Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species and strains. These methods use the variability of nucleic sequences of genes such as 16S rDNA, beta subunit RNA-ase (rpoB), gyrase (gyrB), rDNA internal transcribed spacer and other genes. The aim of this paper is to provide comprehensive information about the methods available to differentiate and identify species of mycobacteria at the DNA sequence level. The methods discussed in the review include PCR, PCR-REA, sequencing analysis, spoligotyping and DNA fingerprinting. These methods have been applied to both the "universal" part...

Hypotheses on the origin and transmission of BSEReview

K. Hruška

Vet Med - Czech, 2001, 46(11):329-332 | DOI: 10.17221/7891-VETMED  

EC Health & Consumer Protection Directorate-General recently published an important document based on papers and reviews discussed by members of TSE/BSE ad hoc group. In this review the Executive Summary and Tables of Contents of Part I and II are printed with permission. For full text and references see the web page mentioned in references.