Veterinární medicína, 2002 (vol. 47), issue 10-11

Comparison of immune parameters in cows with normal and prolonged involution time of uterus

M. Levkut, J. Pistl, V. Revajová, J. Chroma, M. Levkutová, V. Dávid

Vet Med - Czech, 2002, 47(10):277-282 | DOI: 10.17221/5835-VETMED  

Indices of cellular immunity in postpartum Holsteincows with the normal (n = 9) and prolonged (n = 4) uterine involution time were evaluated. Peripheral white blood cells were isolated by lysis from postpartum animals. An indirect immunofluorescence method for staining and flow cytometric analysis was employed to determine the cell subpopulation of lymphocytes. The function assays were also used to examine the activity of lymphocytes and phagocytes. A significant decrease in the lymphocyte absolute number, and subpopulation of T (CD2+, CD4+, CD8+), and B (IgM+) cells in dams with postparturient complications and prolonged uterine...

Effects of some antibiotics on glucose 6-phosphate dehydrogenase in sheep liver

M. Çiftci, V. Turkoglu, S. Aldemir

Vet Med - Czech, 2002, 47(10):283-288 | DOI: 10.17221/5836-VETMED  

In vitro effects of penicillin, sulbactam, cefazolin, and amikacine on the activity of the enzyme glucose-6-phosphate dehydrogenase in sheep liver were investigated. Glucose 6-phosphate dehydrogenase was purified from sheep liver, using a simple and rapid method. The purification consisted of two steps, preparation of homogenate and 2', 5'-ADP Sepharose 4B affinity chromatography. As a result of the two consecutive procedures, the enzyme, having the specific activity of 11.76 EU/mg proteins, was purified with a yield of 35.72% and 1.913 fold. In order to control the enzyme purification SDS polyacrylamide gel electrophoresis (SDS-PAGE) was...

Plasma lipopolysaccharide level and enterocyte brush border enzymes in gnotobiotic piglets infected with Salmonella typhimurium

I. Trebichavský, H. Kozáková, I. Šplíchal

Vet Med - Czech, 2002, 47(10):289-294 | DOI: 10.17221/5837-VETMED  

Gnotobiotic piglets were orally infected either with the virulent LT2 strain or the non-pathogenic SF1591 rough mutant of Salmonella enterica serotype Typhimurium. They were sacrificed 6 or 24 h after the infection. All piglets infected for 24 h developed systemic infection with an increase of plasma lipopolysaccharide. Infection with the virulent strain caused a significant decrease (P < 0.001) of gamma-glutamyl transpeptidase (GGT) activity in the enterocyte brush border of both the jejunum and ileum, infection with the rough mutant caused a decrease of GGT activity in the ileum only. The activities of other brush border...

Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene

S. Indik, L. Valíček

Vet Med - Czech, 2002, 47(10):295-301 | DOI: 10.17221/5838-VETMED  

Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. The high-passage strain V-502 (164) was also differentiated from its parent strain V-502. The same restriction enzymes could distinguish the European-type vaccine strains Porcilis PRRS and Pyrsvac-183, registered inCzechRepublic, from the Czech field isolates. The published...

In vitro susceptibility of duck, chicken, and pig erythrocyte lipids to peroxidation

B. Gradinski-Vrbanac, Z. Stojevič, S. Milinkovič-Tur, T. Balenovič, J. Piršljin, M. Zdeler-Tuk

Vet Med - Czech, 2002, 47(10):303-308 | DOI: 10.17221/5839-VETMED  

The susceptibility of erythrocyte lipid to in vitro peroxidation as measured by TBARS (thiobarbituric acid-reactive substances) and concentration of reduced glutathione (GSH) in whole blood and erythrocyte suspension before and after incubation with hydrogen peroxide was assessed in ducks, chickens and pigs. A high susceptibility of erythrocytes to peroxidation in vitro was observed in all animals tested, but this susceptibility was of different intensity. Pig erythrocytes exhibited the lowest resistance to oxidative stress in vitro as compared with that in ducks (P < 0.01) and chickens (P < 0.02)....

Small mammals - natural reservoir of pathogenic leptospires

F. Treml, M. Pejčoch, Z. Holešovská

Vet Med - Czech, 2002, 47(10):309-314 | DOI: 10.17221/5840-VETMED  

A total of 429 specimens belonging to 9 species of small mammals, trapped in various areas of the CzechRepublicduring the year 2001, were examined for antibodies against leptospires. Antibodies were found in all localities under study in 50 specimens, i.e., 11.6%, belonging to 5 species. Antibodies were most often , i.e., in 42% of positives, found in Microtus arvalis. In Apodemus spp. and Clethrionomys glareolus it was in 34.0% and 14.0% of positives, respectively. In other specimens belonging to Mus musculus and Microtus subterraneus antibodies were found only on occasion. Antibodies against L. grippotyphosa...

Effect of early nutritional experience on the feeding behaviour of adult female rats

Z. Šefčíková, Š. Mozeš

Vet Med - Czech, 2002, 47(10):315-322 | DOI: 10.17221/5841-VETMED  

In the present paper the effect of postnatal undernutrition on feeding behaviour has been evaluated in mature female Wistar rats subjected to food restriction a) during the suckling period, i. e. up to day 15 (SR), b) during the weaning period from day 15 to 30 (WR), c) throughout 30 days post partum (SWR) and d) during adulthood, i.e. from day 60-75 of life (AR). In comparison with the ad libitum fed controls (C), the lactating mothers and the pups of the food-restricted groups were allowed free access to food for only two hours daily. In experimental groups this restriction was then changed to an ad libitum regime. On day 90 food...

Lead intoxication in a puppy - a case report

E. Srebočan, J. Pompe-Gotal, I. Kiš, B. Pirkič, B. Artukovič, Z. Špacir-Prskalo

Vet Med - Czech, 2002, 47(10):323-324 | DOI: 10.17221/5842-VETMED  

Lead intoxication in a puppy is described. The puppy was submitted to surgical procedure for suspicion of acute abdominal pain. Diagnosis was established on the basis of the presence of eroded lead weights in stomach, biochemical and hematological blood tests as well as by elevated tissue lead concentrations.