Veterinární medicína, 2002 (vol. 47), issue 12
The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils
Z. Sládek, D. Vašíčková, D. Ryšánek
Vet Med - Czech, 2002, 47(12):325-332 | DOI: 10.17221/5843-VETMED 
The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apoptosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils...
Functional and phenotypic analyses of porcine gut immune cells immunized by oral administration of F4ac+nonenterotoxigenic Escherichia coli strains
N. Vijtiuk, K. Trutin-Ostovič, T. Balenovič, M. Popovič, I. Valpotič
Vet Med - Czech, 2002, 47(12):333-341 | DOI: 10.17221/5844-VETMED
The aim of this study was to determine the priming effect of experimentally inoculated non-ETEC strains (2407, 1466) on gastrointestinal mucosal lymphocytes. Five 4-week-old pigs per group were orally inoculated with either F4ac+(1466 or 2407) or F4- (1467) non-ETEC strains. The control pigs were given broth containing 1.2% sodium bicarbonate. At postinoculation Day 6 the pigs were killed, their gut lymphocytes were isolated, and their responsiveness was tested in vitro with F4 antigen, peptidoglycan monomer (PGM), pokeweed mitogen (PWM), phytohemagglutinin (PHA) and lipopolysaccharide (LPS). Additionally, the patterns of cluster of differentiation...
Iodine toxicity in ruminants
I. Paulíková, G. Kováč, J. Bíreš, Š. Paulík, H. Seidel, O. Nagy
Vet Med - Czech, 2002, 47(12):343-350 | DOI: 10.17221/5845-VETMED
The inadequate intake of iodine is still a topical problem, because it contributes to a reduction in performance through the disturbed health of adult animals and their offspring. The necessity of iodine is frequently described in association with hypothyroidal state. In our work we want to assign the hidden risk of prolonged iodine feeding in ruminants. We discuss the possible sources of iodine intoxication, the clinical signs of the intoxication, its effects on production, reproduction, and thyroid functions; animal susceptibility to intoxication; laboratory and necropsy findings; diagnostic, and therapeutic possibilities.