Veterinární medicína, 2002 (vol. 47), issue 2-3
Effects of superstimulation with fsh on follicular population and recovery rate of oocytes in the growing phase of the first and second follicular wave
S. Čech, V. Havlíček, M. Lopatářová, M. Vyskočil, R. Doležel
Vet Med - Czech, 2002, 47(2):33-37 | DOI: 10.17221/5800-VETMED 
Effectiveness of in vitro production of embryos (IVP) is limited among other factors by the recovery rate of oocytes. Gonadotropin superstimulation can improve the recovery rate of oocytes. The effect of FSH treatment on follicular population and recovery rate of oocytes at ovum pick-up (OPU) in the growing phase of the 1st as well as the 2nd follicular wave after superstimulation was the object of our experiment. Twelve unpregnant milking cows (15-20 kg milk per day) housed on a dairy farm were used in the experiment. The cows bearing corpus luteum were synchronized by PGF2 (day 0) and they were treated by FSH (Folicotropin inj....
Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis
K. Holovská, V. Lenártová, K. Holovská, P. Javorský
Vet Med - Czech, 2002, 47(2):38-44 | DOI: 10.17221/5801-VETMED
Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron inhibited SOD activity. Changes in Mn-SOD and glutathione peroxidase (GSHPx) activities evoked by paraquat and increased values of TBARS indicated that these enzymes were not able to sufficiently prevent oxidative stress at given paraquat concentrations.
Incidence of bovine tuberculosis in cattle in seven Central European countries during the years 1990-1999
I. Pavlik, W. Yayo Ayele, I. Parmova, I. Melicharek, M. Hanzlikova, B. Körmendy, G. Nagy, Z. Cvetnic, M. Ocepek, N. Fejzic, M. Lipiec
Vet Med - Czech, 2002, 47(2):45-51 | DOI: 10.17221/5802-VETMED
The post-eradication incidence of bovine tuberculosis in seven Central European Countries (Bosnia and Herzegovina, Croatia, Czech Republic, Hungary, Poland, Slovakia and Slovenia) was studied between 1990 and 1999. The majority of cattle to the age of 24 months were screened by tuberculin skin test on annual basis. Tuberculous lesions observed during meat inspection at abattoirs were further laboratory examined by direct microscopy, cultivation and histology for the presence of mycobacteria. Data describing the incidence of the disease in animals for the whole period were obtained from all countries except Bosnia and Herzegovina, where data were obtained...
Occurrence of antibiotic-resistant bacterial strains isolated in poultry
M. Kolář, R. Pantůček, J. Bardoň, I. Vágnerová, H. Typovská, I. Válka, J. Doškář
Vet Med - Czech, 2002, 47(2):52-59 | DOI: 10.17221/5803-VETMED
The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent gramnegatives and grampositives, the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of...
Serological survey of the wild boar (Sus scrofa) for tularaemia and brucellosis in South Moravia, Czech Republic
Z. Hubálek, F. Treml, Z. Juicová, M. Huady, J. Halouzka, V. Janík, D. Bill
Vet Med - Czech, 2002, 47(2):60-66 | DOI: 10.17221/5805-VETMED
Sera of 204 wild boars (Sus scrofa), shot by hunters in the South-Moravian district of Beclav during 1993-2001, were tested by microagglutination reaction using safranin-stained antigens of Francisella tularensis and Brucella abortus: 10.8% and 8.7% seroreactors, respectively, were detected. The highest (17%) prevalence of tularaemia antibodies was found in wild boars during 1993-1994 at the beginning of a widespread outbreak of tularaemia in South Moravia that started in 1994, a nonsignificantly lower (13%) seroprevalence in 1995-1996 during the continuing epizootic, whereas it decreased markedly to 3% in the years 1997-2001...
Experimental toxoplasmosis in hypoiodemic mice
S. Šlosárková, V. Híbalová, I. Literák, I. Herzig, E. Bártová, P. Kodym, M. Malý
Vet Med - Czech, 2002, 47(2):67-74 | DOI: 10.17221/5806-VETMED
The hypothesis, that hypoiodemia of goats causes such a compromise of the immune system, which during subsequent Toxoplasma gondii infections results in clinically more pronounced signs of toxoplasmosis, was verifying in laboratory mouse. Hypoiodemic mice (fed by wheat and supplied by water), normoiodemic mice (fed by wheat and supplied by water containing 1.25 mg KI/l) and the majority of standard mice (fed by commercial grain mixture containing 0.83 mg I/kg) were experimentally infected with T. gondii oocysts or tachyzoites. The susceptibility to acute T. gondii infection was evaluated according to mortality rate. As a...
Animal insulin-like growth factor binding proteins and their biological functions
Z. Kostecká, J. Blahovec
Vet Med - Czech, 2002, 47(2):75-84 | DOI: 10.17221/5807-VETMED
Insulin-like growth factor (IGF-I, IGF-II) action is influenced by until today known eight forms of insulin-like growth factor binding proteins (IGFBPs). They have been obtained not only from some human and animal tissues and body fluids but also from conditioned medium of cell cultures. An important biological property of the IGFBPs is their ability to increase the circulating half-life of the IGFs. They are able to act as potentiators of cell proliferation. As IGFBPs bind to cell surfaces, they may act either to deliver the IGFs to those surfaces for activation of specific receptors or to activate cell responses independently of receptor activation....