Veterinární medicína, 2002 (vol. 47), issue 4
Immunohistochemical characterization of efferent neurons innervating the oviduct in the pig located in the sympathetic chain ganglia
K. Czaja
Vet Med - Czech, 2002, 47(4):85-91 | DOI: 10.17221/5809-VETMED 
The present study was aimed at disclosing the pattern(s) of putative co-incidence of tyrosine hydroxylase (TH), dopamine -hydroxylase (DH), neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and nitric oxide synthase (NOS) within the porcine "oviductal" efferent neurons using combined retrograde tracing and double-labelling immunohistochemistry. The fluorescent retrograde tracer Fast Blue (FB) was injected into the wall of the ampullar (n = 5) and isthmal (n = 5) part of the organ in ten sexually immature female pigs. After a survival period of three weeks sympathetic chain ganglia (SCG) were collected....
Prevalence of serotypes, production of Apx toxins, and antibiotic resistance in strains of Actinobacillus pleuropneumoniae isolated in the Czech Republic
P. Šatrán, K. Nedbalcová
Vet Med - Czech, 2002, 47(4):92-98 | DOI: 10.17221/5810-VETMED
Results of serotyping of 122 strains of Actinobacillus pleuropneumoniae isolated on 21 swine farms in the Czech Republic from January 1999 to December 2000 are presented. The highest prevalence was found for serotype 9 (n = 81, 66.4%), followed by serotype 2 (n = 19, 15.7%). Serotypes 1, 3, 5, 7, 8, 11, and 12 were identified only sporadically. Twenty-six of the isolates were tested for gene-toxic profiles and haemolytic and cytotoxic activities. The investigations confirmed the association between serotype on the one hand and apx genotype and Apx phenotype on the other hand. Results of tests of resistance to antibiotic by...
The utility of the ENTERORapid 24 kit for the identification of P. multocida and M. haemolytica
G. Tefera, J. Smola
Vet Med - Czech, 2002, 47(4):99-103 | DOI: 10.17221/5811-VETMED
ENTERORapid 24 kit (PLIVA-Lachema, Czech Republic) was used for the identification of 321 strains isolated from the respiratory tract of different animal species in the CzechRepublic and Ethiopia. A total of 207 ( 64.5%) strains were identified at the species level within 4 to 8 hours of incubation. In the same way, 39 (12.1%) strains were successfully classified at the genus level. The remaining strains (23.4%) were not identified nor classified to the family Pasteurellaceae. On the other hand, the accuracy of the ENTERORapid 24 kit for the identification of P. multocida and M. haemoyltica was observed using 9 reference...
Brachyspira hyodysenteriae: detection, identification and antibiotic susceptibility
M. Novotná, O. Škardová
Vet Med - Czech, 2002, 47(4):104-109 | DOI: 10.17221/5812-VETMED
101 samples of faeces, colonic mucosa and rectal swabs taken from 100 pigs (29 commercial herds) were cultivated on Trypticase Soy Agar with 5% of sheep blood, spectinomycin (200 mg/l), vancomycin (50 mg/l), rifampicin (12.5 mg/l) and colistin (12.5 mg/l). Plates were incubated in an anaerobic container at 37°C for 5-7 days. 25 samples (10 faeces, 15 scrapings of colonic mucosa) were examined by darkfield microscopy for the presence of spirochaetes. In 80 samples (21 faeces, 31 rectal swabs, 28 scrapings of colonic mucosa) from diarrhoeic pigs 44 isolates of spirochaetes were identified by PCR method as Brachyspira hyodysenteriae. In 21 samples...
Optimal dietary concentration of vitamin E for alleviating the effect of heat stress on performance, thyroid status, ACTH and some serum metabolite and mineral concentrations in broilers
K. Sahin, O. Kucuk, N. Sahin, M. F Gursu
Vet Med - Czech, 2002, 47(4):110-116 | DOI: 10.17221/5813-VETMED
An experiment utilizing Cobb-500 male broilers was conducted to evaluate the effects of vitamin E (d1--to- copheryl acetate) supplementation at various concentrations (0, 62.5, 125, 250, or 500 mg/kg of diet) on performance and serum concentrations of Triiodothyronine (T3), Thyroxin (T4), Adrenocorticotropine Hormone (ACTH), and some metabolites and minerals in broilers reared under heat stress (32°C). One day-old 150 male broilers were randomly assigned to 5 treatment groups, 3 replicates of 10 birds each. The birds received either a basal diet or basal diet supplemented with vitamin E at 62.5, 125, 250, or 500 mg/kg of diet....