Veterinární medicína, 2003 (vol. 48), issue 4
Mycobacterium tuberculosis in animal and human populations in six Central European countries during 1990-1999
I. Pavlik, W. Yayo Ayele, I. Parmova, I. Melicharek, M. Hanzlikova, M. Svejnochova, B. Körmendy, G. Nagy, Z. Cvetnic, V. Katalinic-Jankovic, M. Ocepek, M. Zolnir-Dovc, M. Lipiec, M. Havelkova
Vet Med - Czech, 2003, 48(4):83-89 | DOI: 10.17221/5754-VETMED 
Results of Mycobacterium tuberculosis detection in animals from six Central European countries (Croatia, the Czech Republic, Hungary, Poland, Slovakia and Slovenia) spreading over 610 402 km2 with a population of 11.8 million heads of cattle were analysed. In the monitoring period, 1990 to 1999, M. tuberculosis from animals was isolated only in two countries (Poland and Slovak Republic) from 16 animals with tuberculous lesions. These comprise nine heads of cattle (Bos taurus), four domestic pigs (Sus scrofa f. domestica) and three wild animals, an African elephant (Loxodonta africana),...
Mycobacterium bovis in human population in four Central European countries during 1990-1999
I. Pavlik, W. Yayo Ayele, M. Havelkova, M. Svejnochova, V. Katalinic-Jankovic, M. Zolnir-Dovc
Vet Med - Czech, 2003, 48(4):90-98 | DOI: 10.17221/5755-VETMED
A survey on Mycobacterium bovis and M. tuberculosis in humans has been performed in four Central European countries (Croatia, the Czech Republic, Slovak Republic and Slovenia) during the years 1990 to 1999. These countries cover an area of 204 688 km2 with 22 135 million population. During the period, new cases of tuberculosis were bacteriologically diagnosed in 47 516 patients. M. tuberculosis was detected in 47 461 (99.88%) cases, whereas M. bovis was found only in 55 (0.12%) patients. The rate of infection due to M. bovis in humans did not exceed 0.29% in the study countries. The annual incidence...
Localization of immunoreactivities for neuropeptides and neurotransmitter-synthesizing enzymes in the pterygopalatine ganglion of the pig
P. Podlasz, K. Wąsowicz, J. Kaleczyc, M. Łakomy, R. Bukowski
Vet Med - Czech, 2003, 48(4):99-107 | DOI: 10.17221/5756-VETMED
Study on the presence of the selected biologically active substances in nerve structures of the porcine pterygopalatine ganglion was performed with the use of immunofluorescence and RT-PCR. All neurons in the ganglion were ChAT-, VAChT-, NOS- and VIP- positive. However, some neurons displayed strong immunoreactivity, while in other neurons, immunoreactivity was moderate, or weak. Somatostatin (SOM) was present in approx. 11% of neurons. Tyrosine hydroxylase-positive (TH-positive) neurons were not detected, although in single nerve cell bodies, TH antibody revealed very weak staining which could be attributed to some residual TH immunoreactivity. Immunoreactivity...
Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology
K. Tomanová, J. Klimeš, J. Smola, R. Husník
Vet Med - Czech, 2003, 48(4):108-112 | DOI: 10.17221/5757-VETMED
A nested polymerase chain reaction (PCR) assay and serological examinations were used to detect the presence of Lawsonia intracellularis in a two and a half years old German smooth-coated cocker spaniel with clinical symptoms of chronic diarrhoea and histologically proven inflammatory bowel disease. Fourteen rectal swabs taken over a period of two weeks and eight biopsy specimens taken over a period of six months were used for laboratory examinations. Using the nested PCR, the DNA of L. intracellularis was found in a total of 2 cases, i.e. one rectal swab and one biopsy specimen of the duodenum six months later. The species specificity...