Veterinární medicína, 2005 (vol. 50), issue 12

Qualitative detection of haptoglobin mRNA in bovine and human blood leukocytes and bovine milk somatic cells

M.A. Thielen, M. Mielenz, S. Hiss, H. Sauerwein

Vet Med - Czech, 2005, 50(12):515-520 | DOI: 10.17221/5658-VETMED  

Local expression of haptoglobin (Hp) mRNA in tissue homogenates from different sites of the bovine mammary gland has recently been established. Besides alveolar and stromal tissue, blood cells are one potential cellular location of Hp expression. The aim of this study was to investigate whether bovine leukocytes might contribute to the Hp mRNA transcripts found in the mammary gland after their migration into this organ. For this purpose RT-PCR was carried out with total RNA extracted from leukocytes in blood and from somatic cells in milk of three dairy cows. In comparison, human leukocytes of four donors were examined. Both bovine blood and milk somatic...

The content of iodine in pork

I. Herzig, J. Travnicek, J. Kursa, V. Kroupova

Vet Med - Czech, 2005, 50(12):521-525 | DOI: 10.17221/5659-VETMED  

The present study brings current information on providing market pigs and sows with the required iodine level up to date. The results were obtained by the determination of the iodine content in leg muscles and by the relationship between those concentrations and the iodine intake by the human population. The iodine content was assessed by the Sandell-Kolthoff method in 108 samples of leg muscles (m. gracilis) of market pigs from 18 herds in 10 districts of the Czech Republic collected during the period April 2004 to August 2004. Average iodine content in leg muscles of market pigs was 25.6 ± 15.54 μg I/kg fresh matter, median 20.2...

Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

G. Ozbey, H.B. Ertas, A. Muz

Vet Med - Czech, 2005, 50(12):526-530 | DOI: 10.17221/5660-VETMED  

Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the...

Influence of chronic administration of zearalenone on the processes of apoptosis in the porcine ovary

K. Wasowicz, M. Gajecka, J. Calka, E. Jakimiuk, M. Gajecki

Vet Med - Czech, 2005, 50(12):531-536 | DOI: 10.17221/5661-VETMED  

Zearalenone (ZEA), a micotoxin produced by Fusarium sp. is regarded as a phytoestrogen. Although cytotoxic and genotoxic activity of ZEA was detected, the majority of its toxic influence is related to the ability of binding to estrogen receptors and disrupting the endocrine regulation of the reproductory functions in females. It was previously found that ZEA inhibits proliferation of cells in porcine ovaries, as detected with immunostaining for proliferating cells nuclear antigen (PCNA). The number of PCNA-positive cells was inversely proportional to the dose of ZEA. We decided to answer the question of whether ZEA induces apoptosis in porcine...

The occurrence and dynamics of Ligula intestinalis in its cyprinid fish host, tench, Tinca tinca, in MoganLake (Ankara, Turkey)

M.B. Ergonul, A. Altindag

Vet Med - Czech, 2005, 50(12):537-542 | DOI: 10.17221/5662-VETMED  

Although Ligula intestinalis (L., 1758) has been recorded in several fish hosts, available data on the parasitization parameters of Ligula intestinalis plerocercoids such as prevalence, mean intensity and parasitic index in tench (Tinca tinca L., 1758) is limited. In this study, totally 272 fishes were investigated for the presence of L. intestinalis plerocercoids. The prevalence value was 40.01% of the whole fish sample and the mean intensity was 2.48. The lower IP values and mean intensity levels may provide some evidence of the strategy of Ligula in order to complete its life cycle....

Species level identification of thermotolerant campylobacters

I. Kolackova, R. Karpiskova

Vet Med - Czech, 2005, 50(12):543-547 | DOI: 10.17221/5663-VETMED  

The aim of this study was to compare the phenotypic and genotypic based methods for species identification of thermotolerant campylobacters of human and food origin from the Czech Republic. Phenotypic methods are time-consuming and sometimes lead to intermediate results, therefore replacement by more specific and rapid methods are needed. Out of a total of 911 campylobacter strains tested, 800 human isolates were received from the clinical bacteriology laboratories from 5 regions and 111 foodstuff isolates (raw chicken and pork meat from retail market) originated from the routine examination in our laboratory. Based on the PCR method 85.1% of these...

The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the Czech Republic: a case report

L. Hurkova, D. Halova, D. Modry

Vet Med - Czech, 2005, 50(12):549-552 | DOI: 10.17221/5664-VETMED  

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk...

Malignant fibrous histiocytoma in a dog: a case report

M. M. Kiran, M. Karaman, F. Hatipoglu, Y. Koc

Vet Med - Czech, 2005, 50(12):553-557 | DOI: 10.17221/5665-VETMED  

In this report, a case of malignant fibrous histiocytoma involving skin, lungs, kidneys, pancreas and mediastinal lymph node was described. Microscopically, the tumor classified as storiform-pleomorphic type malignant fibrous histiocytoma.

Surgical therapy of complicated uterine stump pyometra in five bitches: a case report

B. Musal, B. Tuna

Vet Med - Czech, 2005, 50(12):558-562 | DOI: 10.17221/5666-VETMED  

One of the severe complications of ovariohysterectomy is the incomplete removal of one or two ovaries, which is also known as ovarian remnant syndrome. This condition is usually followed by uterine stump pyometra, which can be described as the infection of uterine body tissue remaining after the operation. However, both conditions may also be encountered separately. In this study, the clinical findings, therapies and long term postoperative conditions of five ovarioectomized bitches with uterine stump pyometra that were brought to the clinic with symptoms like lethargy, loss of appetite, purulent or sero-sanguinous vaginal discharge, polydipsia, polyuria...