Veterinární medicína, 2005 (vol. 50), issue 8
Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved
K. Hruska, M. Bartos, P. Kralik, I. Pavlik
Vet Med - Czech, 2005, 50(8):327-335 | DOI: 10.17221/5631-VETMED 
Fifty one products of dried milk baby food purchased from 10 producers from seven countries available on the Czech market have been tested. IS900, the specific fragments for Mycobacterium avium subsp. paratuberculosis (MAP) have been detected using PCR in 25 samples (49.0 %) and fragment f57 by real time PCR in 18 samples (35.3%). These results correspond to the epidemiological situation in Europe and are not unexpected. Paratuberculosis in cattle was almost unknown in the Czech Republic until 1990. An increase in the number of cows with paratuberculosis found in slaughterhouses and the incidence of Crohn's...
An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli
D.Y. Zhang, J.Y. Zhou, J. Fang, J.Q. HU, J.X. WU, A.X. MU
Vet Med - Czech, 2005, 50(8):336-344 | DOI: 10.17221/5632-VETMED
The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the...
Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production
Z. Jaglic, Z. Kucerova, K. Nedbalcova, I. Pavlik, P. Alexa, M. Bartos
Vet Med - Czech, 2005, 50(8):345-354 | DOI: 10.17221/5633-VETMED
The aim of this study was to characterise and compare Pasteurella multocida isolates originating from pigs (n = 43), calves (n = 31), rabbits (n = 27), and to a lesser extent from other hosts (n = 6). A total of 107 P. multocida isolates were obtained from various locations in the Czech Republic. They were analysed by capsular PCR typing and ribotyping, and tested for the production of dermonecrotoxin. Most frequently, serogroup A isolates (n = 74) were found, followed by serogroup D (n = 25) and serogroup F (n = 8) isolates. From a total of fifteen different ribotypes...
Serotype distribution of Actinobacillus pleuropneumoniae isolated from porcine pleuropneumonia in the Czech Republicduring period 2003-2004
Z. Kucerova, Z. Jaglic, R. Ondriasova, K. Nedbalcova
Vet Med - Czech, 2005, 50(8):355-360 | DOI: 10.17221/5634-VETMED
A total of 245 isolates of Actinobacillus pleuropneumoniae included in this study were isolated from lung tissues of pigs which died of porcine pleuropneumonia. The isolates were obtained from 52 swine herds in the Czech Republic over the period 2003-2004. All the serotypes were serotyped by the coagglutination test. All isolates with cross-reactivity between serotypes 9 and 11 or 1, 9, and 11 in the coagglutination test were further serotyped by the quantitative coagglutination test. The results of serotyping showed that serotype 9 was dominant (46.5%) followed by serotypes 2 (18.5%), and 11 (14.2%). Two-way cross-reaction between serotypes...
Peat as a feed supplement for animals: a literature review
M. Trckova, L. Matlova, H. Hudcova, M. Faldyna, Z. Zraly, L. Dvorska, V. Beran, I. Pavlik
Vet Med - Czech, 2005, 50(8):361-377 | DOI: 10.17221/5635-VETMED
Peat is an easily available natural material and a source of biologically active substances widely used, not only in agriculture but in human and animal medicine as well. In recent years, interest in the use of peat as a feed supplement has increased, particularly due to its capability to prevent enteric diseases and to stimulate growth in piglets and pigs. The purpose of this review was to compare the advantages and risks associated with the use of peat for animal nutrition based on the literature available. Beneficial effects of various peat preparations on digestion, growth and the immune systems of animals as well as the absorbent and detoxifying...
Congenital synchronous adenomas of meibomian and moll glands of the eyelid in a calf
H. Yuksel, M.Y. Gulbahar, L. Aslan
Vet Med - Czech, 2005, 50(8):379-383 | DOI: 10.17221/5636-VETMED
This report describes meibomian adenoma (MA) and moll gland adenoma in a female, 1.5-months-old, crossbreed calf. Macroscopically, the mass was 3 × 1.5 × 0.5 cm in size and 5.35 g in weight; its shape was gray-reddish and it was localized on the right lower eyelid. Microscopically, the mass was composed of lobules of various sizes separated with a septum, which included well-differentiated sebaceous cells and apocrine secretory epithelium, with no an association of both gland types.