Vet Med - Czech, 2005, 50(8):336-344 | DOI: 10.17221/5632-VETMED

An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli

D.Y. Zhang1,2, J.Y. Zhou2, J. Fang2, J.Q. HU2, J.X. WU2,3, A.X. MU4
1 College of Life Science,
2 Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
3 Institute of Biotechnology, Zhejiang University, Hangzhou, China
4 General Station of Veterinary Medicine, Hangzhou Bureau of Agriculture, Hangzhou, China

The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure.

Keywords: infectious bronchitis virus; nucleocapsid protein; in vitro expression; ELISA

Published: August 31, 2005  Show citation

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Zhang DY, Zhou JY, Fang J, HU JQ, WU JX, MU AX. An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli. Vet Med - Czech. 2005;50(8):336-344. doi: 10.17221/5632-VETMED.
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