Veterinární medicína, 2006 (vol. 51), issue 3
Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin
E. Skrivanova, M. Marounek, V. Benda, P. Brezina
Vet Med - Czech, 2006, 51(3):81-88 | DOI: 10.17221/5524-VETMED
The antimicrobial activity of fatty acids, monolaurin, citric, succinic, fumaric, malic and lactic acid was determined in cultures of two strains of Escherichia coli, three strains of Salmonella sp. and two strains of Clostridium perfringens. Antimicrobial activity was expressed as minimum inhibitory concentration (MIC) that prevented growth and glucose utilization in treated cultures. Caprylic acid was the only acid inhibiting glucose utilization in all cultures. Its MIC varied from 1 to 3 mg/ml. Strains CCM 3954 and CCM 4225 of E. coli were inhibited also by capric acid at 5 mg/ml. Strains CCM 4435T and...
Appearance of iron-labeled blood mononuclear cells in electron microscopy
D. Horky, I. Lauschova, M. Klabusay, M. Doubek, P. Sheer, S. Palsa, J. Doubek
Vet Med - Czech, 2006, 51(3):89-92 | DOI: 10.17221/5525-VETMED
Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.
Iodine content in consumer hen eggs
J. Travnicek, V. Kroupova, I. Herzig, J. Kursa
Vet Med - Czech, 2006, 51(3):93-100 | DOI: 10.17221/5526-VETMED
This paper presents the latest information about the supply of iodine to meet its requirements in hens of laying type, on the basis of iodine content in egg yolk and about the importance of eggs as an iodine source in human nutrition. The Sandell-Kolthoff method was applied to determine iodine content in the yolk of eggs from 9 large flocks (54 eggs) and 16 small flocks (96 eggs) in 2004, and from 10 large flocks (135 eggs) and 15 small flocks (114 eggs) in 2005. Iodine content was also determined in the albumen of 70 eggs. In 2004, the iodine content in the yolk of eggs from large flocks was 1 014.1 ± 356.6 while in 2005 it amounted to 1 663.8...
Determination of total mercury and mercury species in fish and aquatic ecosystems of Moravian rivers
P. Houserova, V. Kuban, P. Spurny, P. Habarta
Vet Med - Czech, 2006, 51(3):101-110 | DOI: 10.17221/5527-VETMED
Contents of total mercury and mercury species (methylmercury - MeHg, inorganic mercury - Hg2+) were determined in four Moravian rivers - Jihlava, Becva, Loucka and Dyje (Czech Republic). Five tissues (muscle, gills, liver, kidney and skin) of chub (Leuciscus cephalus), zoobenthos, sediments and water samples were analyzed. Time stability of samples was also tested. The highest levels of total mercury were determined in muscle tissues of all tested fish. Relative contents of MeHg in muscle tissues of fish ranged from 83.6% to 92.0% of the total mercury contents. The relative contents of MeHg in sediments and in zoobenthos samples...
A modified salvage technique in surgical repair of perineal hernia in dogs using polypropylene mesh
D. Vnuk, D. Maticic, M. Kreszinger, B. Radisic, J. Kos, M. Lipar, T. Babic
Vet Med - Czech, 2006, 51(3):111-117 | DOI: 10.17221/5528-VETMED
In 16 male dogs who suffered from perineal hernia, polypropylene mesh was used to close a defect in the pelvic diaphragm. Pelvic bone was drilled on the pelvic floor and mesh was sutured through holes by polypropylene suture. Strong pelvic diaphragm, good long-term results and time-sparing by this technique was achieved. Suture sinuses were developed in two dogs one month postoperatively. Objectives of this study were to describe a new alternative technique of perineal herniorraphy and postoperative possible complications. Weakness of internal obturator muscle flap is complication which can be observed during transposition of internal obturator muscle...
Preaxial longitudinal intercalary radial hemimelia in a dog: a case report
M.R. Alam, S.Y. Heo, H.B. Lee, J.H. Kim, Y.J. Park, K.C. Lee, I.H. Choi, N.S. Kim
Vet Med - Czech, 2006, 51(3):118-123 | DOI: 10.17221/5529-VETMED
A 9-month-old 10 kg intact male crossbred dog was admitted to The Animal Medical Centre,ChonbukNationalUniversitywith congenitally malformed right forelimb. Physical exanimation of the limb showed varus deviation of the forepaw and flexion contracture of the interphalangeal joints. The survey radiographs revealed complete absence of the radius, poor congruency of the humeroulnar joint and 90° varus deviation of the ulnocarpal joint. A preaxial longitudinal intercalary radial hemimelia was diagnosed and the dog underwent 3 surgeries for correction of the deformity. During the first surgery, olecranon osteotomy and arthrodesis of the elbow were performed....